结缕草属植物抗寒性的遗传分析

草业学报 ›› 2009, Vol. 18 ›› Issue (3): 53-58.

结缕草属植物抗寒性的遗传分析

郭海林^1,2, 高雅丹^1,2, 薛丹丹^1,2,陈宣²,刘建秀^2*

1.南京农业大学园艺学院,江苏南京 210095;
2.江苏省中国科学院植物研究所南京中山植物园,江苏南京 210014

收稿日期:2008-05-20 出版日期:2009-06-20 发布日期:2009-06-20
作者简介:郭海林(1975-),女,内蒙古乌盟人,助理研究员,在职博士。E-mail: ghlnmg@sina.com
基金资助:
国家自然科学基金项目(30571307)和江苏省科技攻关项目(BE2006339)资助。

Genetic analysis of cold tolerance of zoysia grass

GUO Hai-lin^1,2, GAO Ya-dan^1,2, XUE Dan-dan^1,2, CHEN Xuan², LIU Jian-xiu²

1.College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China;
2.Institute of Botany,
Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China

Received:2008-05-20 Online:2009-06-20 Published:2009-06-20

摘要/Abstract

摘要： 选用结缕草属植物抗寒性两极端类型材料Z136(抗寒)和Z039(不抗寒)相互杂交,获得正反交F₁分离群体,通过改良电导率外渗法对F₁群体的抗寒性进行鉴定,并应用植物数量性状主基因+多基因混合遗传模型分析方法对F₁群体进行遗传分析。结果表明,1)正反交后代的抗寒性变异范围均较大,正交的变异范围为-11.6~-0.3℃, 反交的变异范围为-10.9~-1.7℃,均远远超出了双亲的抗寒性(-9.1和-3.3℃),正反交后代的平均值相差不大,正交后代LT₅₀的平均值为-6.0℃,反交后代LT₅₀的平均值为-6.3℃,均间于双亲之间,没有发现明显的母性遗传现象。2)次数分布分析结果表明,正反交F₁群体的抗寒性均呈混合正态分布,表现出明显的主基因+多基因的遗传特征。3)遗传分析结果表明, 结缕草属植物的最适遗传模型为B-4,即结缕草属植物的抗寒性受2对等加性的主效基因控制,正反交主基因遗传率分别为77.27%和79.60%。本研究目的在于明确结缕草属植物的抗寒性遗传规律,为结缕草属植物抗寒性基因的分子标记、定位以及标记辅助育种提供试验依据。

Abstract: Abstract: The aims of this study were to find the genetic mechanism of cold tolerance in zoysia grass, and to provide an experimental basis for analysis of molecular markers related to cold tolerance for Molecular Marker-Assisted Breeding of zoysia grass. Two Zoysia F₁ segregating populations of the reciprocal crosses of Z136 (cold tolerant) and Z039 ( non-cold tolerant) were obtained, and the cold tolerance of F₁ populations were identified by leaf electrolyte leakage rate. The heredity of cold tolerance in the two F₁ populations of Z136×Z039 and Z039×Z136 were analyzed by major gene and polygene mixed genetic models. Both crosses had a high variation range of cold tolerance: -11.6 to -0.3℃ (Z136×Z039) and -10.9 to -1.7℃ (Z039×Z13), ranges far beyond those of their two parents (-9.1 and -3.3℃). The average LT₅₀ of reciprocal crosses were -6.0 and -6.3℃, both intermediate between the two parents. No obvious maternal genetic phenomenon for cold tolerance were observed in zoysia grass. The frequency distribution of cold tolerance in F₁ populations of the two crosses showed characteristics of a mixed normal distribution, which indicated that the inheritance of cold tolerance was based on a major gene plus poly-genes model. The results of genetic analysis showed that genetic model B-4 was the most suitable for the trait, i.e. the cold tolerance of zoysia was controlled by two equal additive major genes and the heritability of the major genes were 77.27% and 79.60% in the two crosses.

中图分类号:

郭海林, 高雅丹, 薛丹丹,陈宣,刘建秀. 结缕草属植物抗寒性的遗传分析[J]. 草业学报, 2009, 18(3): 53-58.

GUO Hai-lin, GAO Ya-dan, XUE Dan-dan, CHEN Xuan, LIU Jian-xiu. Genetic analysis of cold tolerance of zoysia grass[J]. Acta Prataculturae Sinica, 2009, 18(3): 53-58.

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