添加剂对尖叶胡枝子青贮发酵品质及体外消化率的影响

草业学报 ›› 2009, Vol. 18 ›› Issue (5): 73-79.

添加剂对尖叶胡枝子青贮发酵品质及体外消化率的影响

玉柱¹,魏馨¹,于艳冬²,韩建国¹,孙启忠^3*

1.中国农业大学草地研究所,北京 100193;
2.南京农业大学动物科技学院,江苏南京 210095;

3.中国农业科学院草原研究所,内蒙古呼和浩特 010010

收稿日期:2008-11-25 出版日期:2009-10-20 发布日期:2009-10-20
作者简介:玉柱(1963-),男,蒙古族,内蒙古通辽人, 副教授。E-mail:yuzhu3@sohu.com
基金资助:
公益性行业科研专项“人工草地优质牧草生产技术研究与示范”(nyhyzx07-022),国家科技支撑计划项目“牧草丰产技术集成与产业化示范” (2006BAD16B08)和 “奶牛优质饲草生产技术研究及开发”(2006BAD04-06-06)资助。

Effects of additives on silage quality and in vitro digestibility of Lespedeza hedysaroides silage

YU Zhu¹,WEI Xin¹, YU Yan-dong², HAN Jian-guo¹, SUN Qi-zhong³

1.Institute of Grassland Science, China Agricultural University, Beijing 100193, China;
2.Nanjing
Agricultural University Animal Science and Technology College, Nanjing 210095, China;

3.Grassland Research Institute, CAAS, Huhhot 010010, China

Received:2008-11-25 Online:2009-10-20 Published:2009-10-20

摘要/Abstract

摘要： 以尖叶胡枝子为原料,添加乳酸菌制剂LaLsIL dry(0.000 5%,0.001 0%,0.002 0%和0.007 0%)和Micromanger H/M(0.001 135%,0.003 405%和0.004 450%),在实验室条件下制作小型塑料袋青贮,贮藏90 d,分析乳酸菌制剂对尖叶胡枝子青贮保存性能和消化率的影响。结果表明,添加LaLsIL dry和Micromanger H/M可显著提高胡枝子青贮的乳酸含量(P<0.05),降低丁酸和氨态氮含量 (P<0.05)。通过Flieg青贮饲料评分方案得出尖叶胡枝子直接青贮饲料的发酵品质最差,等级为劣,而2种乳酸菌添加剂处理的青贮品质均明显好于对照组。LaLsIL dry和Micromanger H/M各处理均明显改善了尖叶胡枝子青贮饲料的发酵品质,同时也明显提高了尖叶胡枝子青贮饲料的干物质、粗蛋白质和中性洗涤纤维的体外消化率。

Abstract: Lespedeza hedysaroides silage was made from L. hedysaroides alone or by adding LaLsIL dry (0.000 5%, 0.001 0%, 0.002 0%, 0.007 0%) and Micromanger H/M (0.001 135%, 0.003 405%, 0.004 450%). L. hedysaroides was ensiled in plastic bags under vacuum. After 90 days, samples were analysised for silage quality and in vitro digestibility of L. hedysaroides. The treatments with LaLsIL dry and Micromanger H/M had higher lactic acid contents than the CK (P<0.05) while the content of butyric acid and ammonium nitrogen were lower (P<0.05). The Flieg evaluation method of silage showed that the fermentation quality was worst in directly ensiled L. hedysaroides, and that both treated silages had better fermentation quality than the CK. The treatments of LaLsIL dry and Micromanger H/M improved the fermentation quality and in vitro DM, CP and NDF digestibility of L. hedysaroides silage.

中图分类号:

S816.32

玉柱,魏馨,于艳冬,韩建国,孙启忠. 添加剂对尖叶胡枝子青贮发酵品质及体外消化率的影响[J]. 草业学报, 2009, 18(5): 73-79.

YU Zhu,WEI Xin, YU Yan-dong, HAN Jian-guo, SUN Qi-zhong. Effects of additives on silage quality and in vitro digestibility of Lespedeza hedysaroides silage[J]. Acta Prataculturae Sinica, 2009, 18(5): 73-79.

参考文献

[1]陈宝书.牧草饲料作物栽培学.北京:中国农业出版社,2001.
[2] 刘淑华,松梅,徐丽君,等.2种胡枝子种子生物学特性对比研究.内蒙古草业, 2008,20(3):20-24.
[3] 夏亦荠,苏加楷,熊德邵.二色胡枝子和达乌里胡枝子若干生物学特性和营养成分的分.草业科学,1999,7(1):9-41.
[4] 张铜会,赵哈林,李玉霖,等.科尔沁沙地灌溉与施肥对退化草地生产力的影响.草业学报,1998,17(1):36-42.
[5] 李玉霖,孟庆涛,赵学勇,等.科尔沁沙地流动沙丘植被恢复过程中群落组成及植物多样性演变特征.草业学报,2007,16(6):54-61.
[6] 孙启忠,高丽,赵淑芬,等.尖叶胡枝子.畜牧与饲料科学,2007,9:19-21.
[7] 李荣平,刘志民,闫巧玲.科尔沁沙地西部草甸植物萌发特征.草业学报,2006,15(1):22-28.
[8] Whiter A G, Kung L Jr. The effect of a dry or liquid application of Lactobacillus plantarum MTD1 on the fermentation of alfalfa silage.Dairy Science,2001,84:2195-2202.
[9] 庄益芬,安宅一夫,张文昌.不同温度条件下添加剂对青贮苜蓿细胞壁成分和体外干物质消化率的影响.家畜生态学报,2006,27(6):61-64.
[10] Koehler L H, Differentiation of carbohydrate by anthrone reaction rate and colour intensity. Analytical Chemistry, 1952, 24: 1576-1579.
[11] Krishnamoorthy U, Muscato T V, Sniffen C J, et al. Nitrogen fractions in selected feedstuffs. Journal Dairy Science, 1982, 65: 217-225.
[12] Playne M J, McDonald P. The buffering constituents of herbage and silage. Journal Science Food Agriculture, 1966,17:264-268.
[13] Broderica G A, Kang J H. Automated simultaneous determination of ammonia and amino acids in ruminal fluid and in vitro media. Journal Dairy Science, 1980,33:64-75.
[14] Van Soest P J, Robertson J B, Lewis B A. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal Dairy Science, 1991, 74: 3583-3597.
[15] 许庆方,玉柱,韩建国,等.高效液相色谱法测定紫花苜蓿青贮中的有机酸.草原与草坪,2007,2:63-67.
[16] 张子仪.中国饲料学. 北京:中国农业出版社, 2000.
[17] Tilly J M A, Terry R A. A two stage technique for the in vitro digestion of forage crops. Journal British Grassland Society, 1963,18: 104-111.
[18] McDougall E I. Studies on ruminant salive I. The composition and output of sheep’s salive. Biochemical Journal, 1948,43:99-109.
[19] 刘建新,杨振海.青贮饲料的合理调制与质量评定标准.饲料工业,1999,20(3):4-7.
[20]Tengerdy R P, Weinberg Z G, Szakacs G, et al. Ensiling alfalfa with additives of lactic acid bacteria and enzymes. Journal of the Science of Food and Agriculture, 1991,55:215-228.
[21] 玉柱,白春生,孙启忠,等. 不同添加剂对无芒雀麦青贮品质的影响.中国农业科技导报,2008,10(4):76-81.
[22] 玉柱,孙启忠,邓波,等.老芒麦青贮研究.中国农业科技导报,2008,10(1):98-102.
[23] Aksu T,Baytok E,Bolat D. Effects of abacterial silage inoculant on corn silage fermentation and nutrient digestibility.Small Ruminant Research,2004,55:249-252.
[24] Harrison J H, Soderlund S D,Loney K A. Effect of inoculation rate of selecte dstrains of lactic acid bacteria on fermentation and in vitro digestibility of grass-legume forage. Journal Dairy Science, 1989,72(9):2421-2426.