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草业学报 ›› 2009, Vol. 18 ›› Issue (6): 164-168.

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高能混合粒子场处理种子对龙牧803苜蓿M2代的抗寒性影响

尚晨1,韩贵清2*,陈积山2,刘杰琳2,张月学2,
唐凤兰2,韩微波2,蒿若超2,刘凤歧2   

  1. 1.哈尔滨师范大学,黑龙江 哈尔滨 150086;
    2.黑龙江省农业科学院草业所,黑龙江 哈尔滨 150086
  • 收稿日期:2009-01-12 出版日期:2009-12-20 发布日期:2009-12-20
  • 作者简介::尚晨(1982-),男, 黑龙江哈尔滨人,在读博士。

Effects on cold tolerance of M2 of Longmu No. 803 alfalfa seed pre-treatedwith a mixed high-energy particle field

SHANG Chen1, HAN Gui-qing2, CHEN Ji-shan2, LIU Jie-lin2, ZHANG Yue-xue2,
TANG Feng-lan2, HAN Wei-bo2, HAO Ruo-chao2, LIU Feng-qi2   

  1. 1.Harbin Normal University, Harbin 150086, China;
    2.Institute of Pratacultural Science,
    Heilongjiang Academy of Agricultural Science, Harbin 150086, China
  • Received:2009-01-12 Online:2009-12-20 Published:2009-12-20

摘要: 利用高能粒子处理紫花苜蓿干种子,观测龙牧803苜蓿M2代在秋末冬初低温条件下叶片游离脯氨酸含量(Pro)、过氧化物歧化酶活性(SOD)、可溶性糖含量(LSSC)、叶绿素a含量(Chla)和叶绿素b含量(Chlb)。结果表明, 高能粒子处理在秋末冬初低温条件下M2代的抗寒性指标显著高于对照(P<0.05);随着辐照剂量的增大,M2代叶片Pro含量、SOD酶活性、LSSC含量、Chl含量明显递减。低剂量辐照下M2代获得的抗寒性强于高剂量辐照,由此确定苜蓿的最佳高能粒子辐照剂量为145 Gy,其次是195 Gy。

Abstract: The seeds of Longmu No. 803 alfalfa were pre-treated in a mixed high-energy particle field. The effect of M2 on Longmu No. 803 alfalfa subjected to low temperature treatment between autumn and winter were studied, including monitoring the leaf for free proline content, SOD activity, LSSC, Chla and Chlb contents after treatment with different radiant dosages. The mixed high-energy particle field significantly (P<0.05) increased the cold tolerance of M2 compared with the control. With increasing radiant dosage, the proline content, SOD activity, LSSC, Chla and Chlb contents of M2 were all significantly reduced. The low radiant dosage induced stronger cold tolerance than the high radiant dosage. There were two options involving 145 or 195 Gy of mixed high-energy particle field intensity.

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