不同水分处理下紫花苜蓿刈割后残茬的光合变化及其机制

草业学报 ›› 2009, Vol. 18 ›› Issue (6): 192-197.

不同水分处理下紫花苜蓿刈割后残茬的光合变化及其机制

何树斌,刘国利,杨惠敏^*

兰州大学草地农业科技学院农业部草地农业生态系统学重点开放实验室,甘肃兰州730020

收稿日期:2008-11-05 出版日期:2009-12-20 发布日期:2009-12-20
作者简介:何树斌(1983-),男,甘肃武威人,在读博士。E-mail: heshubin215@163.com
基金资助:
973计划课题(2007CB108901),教育部高等学校博士学科点专项科研基金(20070730032)和兰州大学引进人才计划课题资助。

Changes and mechanism in response of photosynthetic rates of lucerne residueto cutting under different water treatments

HE Shu-bin, LIU Guo-li, YANG Hui-min

Key Laboratory of Grassland Agro-ecosystems, Ministry of Agriculture；
School of Pastoral
Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China

Received:2008-11-05 Online:2009-12-20 Published:2009-12-20

摘要/Abstract

摘要： 以紫花苜蓿为研究对象,测定了不同水分处理下牧草刈割后残茬光合作用、膜脂过氧化、渗透调节物质及抗氧化酶类物质的变化,初步探讨了刈割后紫花苜蓿光合变化的机制。刈割后较短时间内紫花苜蓿光合速率(Pn)可恢复甚至上升,气孔导度(gs)也有不同程度的增加;刈割后10 h,紫花苜蓿丙二醛(MDA)含量下降明显,而脯氨酸(proline)含量增加显著;抗氧化酶(SOD、POD和 CAT)活性在刈割后总体迅速上升。不同水分处理下有相同变化趋势,但不同水分处理间差异显著。刈割可能缓解了水分胁迫,减弱了膜脂过氧化程度;渗透调节物质增多,增强了抵抗“刈割”刺激/胁迫的能力;抗氧化酶活性的总体增高,提高了清除“刈割”产生的活性氧的能力,有助于维护光合细胞膜的完整性。总之,细胞膜脂过氧化的减弱、活性氧清除的加强和抵抗胁迫能力的提高,保证了植物器官功能(如气孔运动)的正常甚至超常发挥,从而导致刈割后紫花苜蓿残茬迅速恢复甚至提高光合能力。

Abstract: The changes in photosynthetic rate and its mechanism during lucerne (Medicago sativa) regrowth after cutting were investigated in a greenhouse experiment. Photosynthetic rate (Pn), stomatal conductance (gs), contents of malondialchehyche (MDA) and proline, and the activities of superoxidase (SOD), peroxidase (POD) and catalase (CAT) were measured at different soil water availabilities and cutting. Pn and gs increased shortly after cutting. Leaf MDA content decreased 10 h after cutting, while proline content increased. In addition, activity of antioxidases, including SOD, POD and CAT, increased shortly after cutting. The same trend of changes was observed under both water treatments, but significant difference appeared between treatments. It is suggested that cutting stimuli may reduce water stress within the plant body, enhancing osmotic adjustment and clearance of oxygen species, promoting normal functions of plant organs like stomatal movement. Thus, enough CO₂ can be supplied and Pn is then maintained and even enhanced.

中图分类号:

何树斌,刘国利,杨惠敏^*. 不同水分处理下紫花苜蓿刈割后残茬的光合变化及其机制[J]. 草业学报, 2009, 18(6): 192-197.

HE Shu-bin, LIU Guo-li, YANG Hui-min. Changes and mechanism in response of photosynthetic rates of lucerne residueto cutting under different water treatments[J]. Acta Prataculturae Sinica, 2009, 18(6): 192-197.

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