四川盆地麦冬种质资源的过氧化物同工酶分析

草业学报 ›› 2009, Vol. 18 ›› Issue (6): 259-263.

四川盆地麦冬种质资源的过氧化物同工酶分析

刘江,陈兴福^*,杨文钰,张树平,刘卫国,赵雪莉

四川农业大学农学院,四川雅安 625014

收稿日期:2009-02-25 出版日期:2009-12-20 发布日期:2009-12-20
作者简介:刘江(1986-),男,四川江油人,在读硕士。
基金资助:
四川省育种攻关项目(No.2006yzggl2-12) 资助

Peroxidase isozyme variations among germplasm resources ofOphiopogon japonicus in the Sichuan Basin

LIU Jiang, CHEN Xing-fu, YANG Wen-yu, ZHANG Shu-ping, LIU Wei-guo, ZHAO Xue-li

College of Agronomy, Sichuan Agricultural University, Ya’an 625014, China

Received:2009-02-25 Online:2009-12-20 Published:2009-12-20

摘要/Abstract

摘要： 为了探讨不同产地25份麦冬样品间的亲缘关系及与其外观性状的关系,采用聚丙烯酰胺凝胶电泳技术,进行过氧化物(POD)同工酶分析,并使用SPSS软件对谱带作聚类分析。结果显示,不同产地麦冬样品的POD同工酶有10种酶谱类型,每种POD酶谱类型具有6～11条酶带;不同株型麦冬的酶谱类型相同;来源相同,外观性状不同的麦冬样品,其POD同工酶酶谱类型不同。因此,四川盆地麦冬种质资源具有丰富的遗传多样性,其遗传差异与地理分布联系并不紧密,而与形态特征联系较紧;过氧化物同工酶分析可以作为川产麦冬种质资源鉴定的重要参考依据,并可用于判断其遗传差异及亲缘关系。

Abstract: This study explored the relationships between genetic and morphological characteristics of Ophiopogon japonicus using 25 samples collected from different areas of the Sichuan Basin. Peroxidase isozyme (POD) was studied using vertical slab polyacrylamide gel electrophoresis (PAGE) and the zymograms were analyzed using SPSS software version 13.0. There were 10 different POD band types and each band type was composed of 6 to 11 bands. There was no significant difference in POD band type between erective and creepy morphotypes. The band type was different between materials collected in the same area but with different morphological characteristics. There were plentiful POD variations among the germplasm resources of O. japonicus in the Sichuan Basin. The genetic relationship was not associated with geographical distributions, but was obviously related to the morphological characteristics. POD characteristics could be considered as a significant reference base in the classification of the germplasm materials of O. japonicus, and could be used to determine the genetic difference and relationships of the germplasm materials.

中图分类号:

刘江,陈兴福^*,杨文钰,张树平,刘卫国,赵雪莉. 四川盆地麦冬种质资源的过氧化物同工酶分析[J]. 草业学报, 2009, 18(6): 259-263.

LIU Jiang, CHEN Xing-fu, YANG Wen-yu, ZHANG Shu-ping, LIU Wei-guo, ZHAO Xue-li. Peroxidase isozyme variations among germplasm resources ofOphiopogon japonicus in the Sichuan Basin[J]. Acta Prataculturae Sinica, 2009, 18(6): 259-263.