转BADH基因苜蓿T1代遗传稳定性和抗盐性研究

草业学报 ›› 2009, Vol. 18 ›› Issue (6): 65-71.

转BADH基因苜蓿T1代遗传稳定性和抗盐性研究

燕丽萍¹,夏阳^1* ,梁慧敏²,王珍珍¹,李双云¹,刘翠兰¹,庞彩红¹

1.山东省林业科学研究院,山东济南 250014;
2.江苏农林职业技术学院,江苏句容 212400

收稿日期:2009-02-05 出版日期:2009-12-20 发布日期:2009-12-20
作者简介:燕丽萍(1980-),女,甘肃定西人,工程师,硕士。E-mail:ylp_982@163.com
基金资助:
山东省良种工程项目(2006lz12-02)资助

A study on salt tolerance and the genetic stability of T1 generation transgenic alfalfa with the BADH gene

YAN Li-ping¹, XIA Yang¹, LIANG Hui-min², WANG Zhen-zhen¹,
LI Shuang-yun¹, LIU Cui-lan¹, PANG Cai-hong¹

1.Shandong Provincial Academy of Forestry, Jinan 250014, China；
2.Jiangsu Agriculture and
Forestry Profession Technology College, Jurong 212400, China

Received:2009-02-05 Online:2009-12-20 Published:2009-12-20

摘要/Abstract

摘要： 采用分子生物学检测和不同浓度的NaCl对转BADH基因苜蓿T1代2个株系进行遗传稳定性和抗盐性研究。结果表明,2个株系的T1代PCR阳性植株和阴性植株分离比例都符合孟德尔遗传规律;在0.8%NaCl胁迫下,转基因植株Northern杂交表达量明显增加,BADH酶活性和甜菜碱含量分别比对照植株增加2～4和4～5倍;不同浓度NaCl胁迫下转基因株系脯氨酸和可溶性糖含量、抗氧化酶活性都显著高于对照植株,而电导率和丙二醛含量显著低于对照植株。表明转入的BADH基因可以稳定遗传,转基因苜蓿植株耐盐性明显高于对照。

Abstract: The physiological response of transgenic alfalfa to different salt stresses and its PCR detection were investigated. The ratio of negative to positive numbers of PCR detection confirmed the first Mendelian law. The transgenenic T1 generations showed enhanced BADH activity and betaine content, compared to the non-transgenenic plants at 0.8% NaCl stress. Proline and soluble sugar contents and the antioxidase activity of transgenic T1 generations were higher than those of non-transgenic plants at different salt stresses, while electrolyte leakage and malonaldehyde contents were lower. This suggests that the BADH gene was expressed in the transgenic T1 generation, resulting in improved salt tolerance in the transgenic plants.

燕丽萍,夏阳 ,梁慧敏²,王珍珍,李双云,刘翠兰,庞彩红. 转BADH基因苜蓿T1代遗传稳定性和抗盐性研究[J]. 草业学报, 2009, 18(6): 65-71.

YAN Li-ping, XIA Yang, LIANG Hui-min, WANG Zhen-zhen,
LI Shuang-yun, LIU Cui-lan, PANG Cai-hong. A study on salt tolerance and the genetic stability of T1 generation transgenic alfalfa with the BADH gene[J]. Acta Prataculturae Sinica, 2009, 18(6): 65-71.

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