信号分子H2O2调节抗氧化系统提高高羊茅耐热性研究

草业学报 ›› 2010, Vol. 19 ›› Issue (1): 89-94.

信号分子H₂O₂调节抗氧化系统提高高羊茅耐热性研究

王艳,李建龙^*,余醉,薛峰

南京大学生命科学院,江苏南京 210093

收稿日期:2009-02-20 出版日期:2010-01-25 发布日期:2010-02-20
作者简介:王艳(1978-),女,山西襄汾人,博士。E-mail: wangyan7826@sohu.com
基金资助:
国家高科技研究发展计划(863计划)项目(2007AA10Z231)和南京大学博士后科研启动基金(0208003089)资助。

The signaling molecule H₂O₂ improved the heat-tolerance system of Festuca arundinaceaby up-regulating antioxidative activity

WANG Yan, LI Jian-long, YU Zui, XUE Feng

School of Life Science, Nanjing University, Nanjing 210093, China

Received:2009-02-20 Online:2010-01-25 Published:2010-02-20

摘要/Abstract

摘要： 采用盆栽试验,利用10 mmol/L的H₂O₂对冷季型草坪草高羊茅进行叶面喷施处理,研究外源低浓度H₂O₂对高羊茅叶片中抗氧化系统的调控作用及其对高羊茅抗热性的影响。结果表明,H₂O₂可能作为信号分子预先增加抗氧化酶的活性,改变抗氧化剂的浓度,从而减轻随后发生的热胁迫对草坪草造成的氧化伤害;在胁迫过程中POD和CAT活性在H₂O₂预处理后增加不显著,热胁迫本身增加了POD的活性,但是降低了CAT活性,POD对于提高高羊茅的耐热性可能具有更重要的作用;外源H₂O₂显著影响了高羊茅叶片中的AsA-GSH循环,其中处理植株中的APX、GPX和GR的活性在热胁迫过程中增加20%～110%,GSH/GSSG下降了80%,与高羊茅抗热性的提高密切相关。可见信号分子H₂O₂可以通过调控高羊茅的抗氧化系统提高其抗热性。

Abstract: Cool-season turfgrass Festuca arundinacea was pretreated with 10 mmol/L H₂O₂ in pot experiments, and the regulation of the antioxidative system by exogenous H₂O₂ and its effects on heat-tolerance were studied. Exogenous H₂O₂ may activate the defensive system in F. arundinacea ahead of heat stress, and alleviate oxidative damage resulting from subsequent heat stress. CAT and POD activities were not significantly increased by exogenous H₂O₂ treatment. Heat stress itself increased POD activity, and POD may play a more important role in improving heat resistance of the turfgrass. H₂O₂ significantly affected the AsA-GSH cycle in F. arundinacea. APX, GPX and GR activities increased 20%-110% and GSH/GSSG significantly decreased 80% in the cycle, and were closely associated with the acquirement of heat tolerance in F. arundinacea. The signaling molecule H₂O₂ can improve the heat-tolerance of F. arundinacea by regulating the antioxidative system.

中图分类号:

王艳,李建龙^*,余醉,薛峰. 信号分子H₂O₂调节抗氧化系统提高高羊茅耐热性研究[J]. 草业学报, 2010, 19(1): 89-94.

WANG Yan, LI Jian-long, YU Zui, XUE Feng. The signaling molecule H₂O₂ improved the heat-tolerance system of Festuca arundinaceaby up-regulating antioxidative activity[J]. Acta Prataculturae Sinica, 2010, 19(1): 89-94.

参考文献

[1] 李建龙, 晏笳, 蒋平, 等. 亚热带主要草坪草抗性生理研究进展[J]. 中国草地, 2002, 24(4): 41-46.
[2] Larkindale J, Huang B R. Thermotolerance and antioxidant systems in Agrostis stolonifera: Involvement of salicylic acid, abscisic acid, calcium, hydrogen peroxide, and ethylene[J]. Journal of Plant Physiology, 2004, 161: 405-413.
[3] 苏桐, 龙瑞军, 魏小红, 等. 外源NO对NaCl胁迫下燕麦幼苗氧化损伤的保护作用[J]. 草业学报, 2008, 17(5): 48-53.
[4] 曲涛, 南志标. 作物和牧草对干旱胁迫的响应及机理研究进展[J]. 草业学报, 2008, 17(2): 126-135.
[5] Huang S H, Yu C W, Lin C H. Hydrogen peroxide functions as a stress signal in plants[J]. Botanical Bulletin of Academia Sinica, 2005, 46(1): 1-10.
[6] Andre-Dias de A N, Jose-Tarquinio P, Joaquim E F, et al. Hydrogen peroxide pre-treatment induces saltstress acclimation in maize plants[J]. Journal of Plant Physiology, 2005, 162: 1114-1122.
[7] Wahid A, Perveen M, Gelani S, et al. Pretreatment of seed with H₂O₂ improves salt tolerance of wheat seedlings by alleviation of oxidative damage and expression of stress proteins[J]. Journal of Plant Physiology, 2007, 164(3): 283-294.
[8] 冉飞, 包苏科, 石丽娜, 等. 干旱胁迫和复水对锡金微孔草抗氧化酶系统的影响[J]. 草业学报, 2008, 17(5): 156-160.
[9] Dagmar P, Sairam R K, Srivastava G C, et al. Oxidative stress and antioxidant activity as the basis of senescence in maize leaves[J]. Plant Science, 2001, 161: 765-771.
[10] 李忠光, 杜朝昆, 龚明. 在单一提取系统中同时测定植物AsA/DHA和GSH/GSSG[J]. 云南师范大学学报, 2003, 23(3): 67-70.
[11] Smith I K, Vierheller T L, Thorne C A. Assay of glutathione reductase in crude homogenates use 5, 5′-dithiol-bis(2-nitrobenzoic acid)[J]. Analytical Biochemistry, 1988, 175: 408-413.
[12] Cheng Y L, Song C P. Hydrogen peroxide homeostasis and signaling in plant cells[J]. Science China Series C-Life Sciences, 2006, 49(1): 1-11.
[13] Horváth E, Pál M, Szalai G, et al. Exogenous 4-hydroxybenzoic acid and salicylic acid modulate the effect of short-term drought and freezing stress on wheat plants[J]. Biologia Plantarum, 2007, 51: 480-487.
[14] Bradley D J, Kjellbon P, Lamb C J. Elicitor- and wound-induced oxidative cross-linking of a proline-rich plant cell wall protein: A novel, rapid defense response[J]. Cell, 1992, 70(1): 21-30.
[15] Anderson M D, Prasad T K, Stewart C R. Changes in isozyme profiles of catalase peroxidase and glutathione reductase during acclimation to chilling in mesocotyls of maize seedling[J]. Plant Physiology, 1995, 109: 1247-1257.
[16] Elbieta K, Maria S.Ascorbate, glutathione and related enzymes in chloroplasts of tomato leaves infected by Botrytis cinerea[J]. Plant Science, 2001, 160: 723-731.
[17] Kocsy G, Galiba G, Brunold C. Role of glutathione in adaptation and signalling during chilling and cold acclimation in plants[J]. Physiologia Plantarum, 2001, 113: 58-164.
[18] Milla M A R, Maurer A, Huete A R, et al. Glutathione peroxidase genes in Arabidposis are ubiquityous and regulated by abiotic stresses though diverse signaling pathways[J]. The Plant Journal, 2003, 36: 602-615.
[19] Edwards R, Dixon D P, Walbot V. Plant glutathione S-transferases enzymes with multiple functions in sickness and in health[J]. Trends in Plant Science, 2000, 5: 193-198.

信号分子H₂O₂调节抗氧化系统提高高羊茅耐热性研究

The signaling molecule H₂O₂ improved the heat-tolerance system of Festuca arundinaceaby up-regulating antioxidative activity

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

[1]	郭郁频，米福贵，闫利军，任永霞，吕世杰，伏兵哲. 不同早熟禾品种对干旱胁迫的生理响应及抗旱性评价[J]. 草业学报, 2014, 23(4): 220-228.
[2]	杨海霞，刘润进，郭绍霞. AM真菌摩西球囊霉对盐胁迫条件下高羊茅生长特性的影响[J]. 草业学报, 2014, 23(4): 195-203.
[3]	韩朝,刘洋,董慧,常智慧. 污泥对高羊茅抗旱性的影响研究[J]. 草业学报, 2014, 23(3): 127-135.
[4]	张怀山,赵桂琴,栗孟飞,夏曾润,王春梅. 中型狼尾草幼苗对PEG、低温和盐胁迫的生理应答[J]. 草业学报, 2014, 23(2): 180-188.
[5]	彭燕,李州. 干旱预处理对抗旱性不同的2个草地早熟禾品种耐热性能的影响[J]. 草业学报, 2013, 22(5): 229-238.
[6]	俞乐,刘拥海,周丽萍,梁国球. 干旱胁迫下结缕草叶片抗坏血酸与相关生理指标变化的品种差异研究[J]. 草业学报, 2013, 22(4): 106-115.
[7]	陈敏,侯新村,范希峰,武菊英,潘远智. 细叶芒苗期耐旱性分析[J]. 草业学报, 2013, 22(3): 184-.
[8]	蒋乔峰,陈静波,宗俊勤,李珊,褚晓晴,郭海林,刘建秀. 盐胁迫下磷素对沟叶结缕草生长及Na⁺和K⁺含量的影响[J]. 草业学报, 2013, 22(3): 162-.
[9]	李晓宇,蔺吉祥,李秀军,穆春生. 羊草苗期对盐碱胁迫的生长适应及Na⁺、K⁺代谢响应[J]. 草业学报, 2013, 22(1): 201-209.
[10]	陈静波,刘建秀. 狗牙根抗盐性评价及抗盐机理研究进展[J]. 草业学报, 2012, 21(5): 302-310.
[11]	李珊,陈静波,郭海林,宗俊勤,张芳,禇晓晴,蒋乔峰,丁万文,刘建秀. 结缕草属草坪草种质资源的耐盐性评价[J]. 草业学报, 2012, 21(4): 43-51.
[12]	袁学军,王志勇,郑轶琦,刘建秀,佘建明. 假俭草体细胞抗寒突变体的获得及其SRAP分子鉴定[J]. 草业学报, 2011, 20(6): 237-244.
[13]	王丹,宣继萍,郭海林,刘建秀. 结缕草的抗寒性与体内碳水化合物、脯氨酸、可溶性蛋白季节动态变化的关系[J]. 草业学报, 2011, 20(4): 98-107.
[14]	董秋丽,夏方山,董宽虎. NaCl胁迫对芨芨草苗期脯氨酸代谢的影响[J]. 草业学报, 2010, 19(5): 71-76.
[15]	王艳,李建龙,姜涛,邓蕾. SA、H₂O₂和6-BA预处理对沟叶结缕草耐寒性的影响[J]. 草业学报, 2010, 19(2): 76-81.