外源一氧化氮供体SNP对NaCl胁迫下黑麦草幼苗叶片抗坏血酸-谷胱甘肽循环的影响

摘要/Abstract

摘要： 采用营养液栽培,研究了外源一氧化氮(NO)供体硝普钠(SNP)对150mmol/LNaCl胁迫下黑麦草幼苗叶片抗坏血酸-谷胱甘肽(ASA-GSH)循环中抗氧化酶活性和抗氧化物质及丙二醛(MDA)和H₂O₂含量的影响。结果表明,正常条件下100μmol/LSNP略微降低了黑麦草幼苗叶片的MDA和H₂O₂含量,NO信号转导途径关键酶鸟苷酸环化酶(GC)抑制剂亚甲基蓝(MB)促进了MDA和H₂O₂水平的提高。NaCl胁迫下,SNP显著缓解了MDA和H₂O₂的积累,提高了抗坏血酸过氧化物酶(APX)、谷胱甘肽还原酶(GR)和脱氢抗坏血酸还原酶(DHAR)活性及还原型抗坏血酸(ASA)、谷胱甘肽(GSH)含量,降低脱氢抗坏血酸(DHA)和氧化型谷胱甘肽(GSSG)含量,使ASA/DHA和GSH/GSSG提高,却对单脱氢抗坏血酸还原酶(MDAR)活性无显著影响;MB逆转了SNP对NaCl胁迫下MDA、H₂O₂、ASA、GSH、DHA、GSSG含量和APX、GR活性及ASA/DHA和GSH/GSSG的调节作用,对MDAR和DHAR活性无显著影响。由此表明,NO可能通过GC介导参与盐胁迫下黑麦草叶片ASA-GSH循环中APX、GR活性和ASA、GSH含量及ASA/DHA、GSH/GSSH的调节,缓解盐胁迫诱导的氧化伤害,提高植株的耐盐性。

Abstract: Effects of the exogenous NO donor sodium nitroprusside (SNP) on the ascorbate-glutathione cycle dependent antioxidant enzyme activities, contents of antioxidant substance, H₂O₂ and malondialdehyde (MDA) in leaves of hydroponically-grown perennial ryegrass (Lolium perenne) seedlings under 150 mmol/L NaCl stress were investigated. The results showed that 100 μmol/L SNP slightly reduced the MDA and H₂O₂ contents of ryegrass seedling leaves under normal growth conditions, but exogenous methylene blue (MB), the inhibitor of the key enzyme guanylate cyclase (GC) during signal transfer of NO, increased the MDA and H₂O₂ contents. Exogenous SNP significantly alleviated MDA and H₂O₂ accumulations in ryegrass seedling leaves under NaCl stress, while the activities of APX, GR and DHAR, the contents of ASA and GSH, and the ratios of ASA/DHA and GSH/GSSG were increased. Under NaCl stress, the DHA and GSSG contents were reduced while the activity of MDAR was not affected. MB reversed some effects of SNP under NaCl stress, such as increasing the levels of MDA and H₂O₂, but reducing the activities of APX and GR, the contents of ASA and GSH, and the ratios of ASA/DHA and GSH/GSSG. The DHA and GSSG contents were increased but the activities of MDAR and DHAR were not significantly affected under NaCl stress. These findings suggested that NO might be involved in regulation of ascorbate-glutathione cycle metabolism dependent on the activities of APX and GR, the contents of ASA and GSH, and the ratios of ASA/DHA and GSH/GSSG through the GC pathway thus alleviating the oxidative damage to ryegrass seedling leaves from salt stress and improving salt resistance of the perennial ryegrass plants.

中图分类号:

刘建新, 王鑫, 李博萍. 外源一氧化氮供体SNP对NaCl胁迫下黑麦草幼苗叶片抗坏血酸-谷胱甘肽循环的影响[J]. 草业学报, 2010, 19(2): 82-88.

LIU Jian-xin, WANG Xin, LI Bo-ping. Effects of exogenous nitric oxide donor SNP on ascorbate-glutathionecycle metabolism in ryegrass seedling leaves under NaCl stress[J]. Acta Prataculturae Sinica, 2010, 19(2): 82-88.

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