甘肃贝母种子灌浆特性研究

草业学报 ›› 2010, Vol. 19 ›› Issue (2): 97-102.

甘肃贝母种子灌浆特性研究

郭凤霞^1,2,3,常彦莉^2,3,林玉红⁴,王三喜⁵,朱君⁵,陈垣^2,3*

1.中国科学院寒区旱区环境与工程研究所极端环境生物抗逆特性与生物技术实验室,甘肃兰州 730000;
2.甘肃省作物遗传
改良与种质创新重点实验室,甘肃兰州 730000;
3.甘肃农业大学农学院和生命科学技术学院,甘肃兰州 730070;

4.甘肃省农业科学院生物技术研究所,甘肃兰州 730070;
5.甘南州农业科学研究所,甘肃甘南 747000

收稿日期:2009-09-15 出版日期:2010-02-25 发布日期:2010-04-20
作者简介:郭凤霞(1963-),女,甘肃陇西人,研究员,博士。E-mailcygcx@yahoo.com.cn
基金资助:
兰州市科技局项目——甘肃贝母脱毒快繁技术开发(06-02-60)和甘南州财政局项目——甘南藏(中)药材川贝母人工种植示范项目资助。

Studies on grain filling characteristics of Fritillaria przewalskii

GUO Feng-xia^1,2,3,CHANG Yan-li^2,3,LIN Yu-hong⁴,WANG San-xi⁵,ZHU Jun⁵,CHEN Yuan^2,3

1.Laboratory of Extreme Environmental Biology Adversity Tolerance and Biotechnology, Cold and Arid
Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou
730000, China;
2.Gansu Key Laboratory of Crop Genetic &
Germplasm Enhancement, Lanzhou
730070, China;
3.Colleges of Agronomy, Colleges of Life Sciences and Technology, Gansu
Agriculture University, Lanzhou 730070, China;
4.Institute of Biotechnology, Gansu
Academy of Agricultural Sciences, Lanzhou 730070, China;
5.Gannanzhou
Institute of Agricultural Sciences, Gannan 747000, China

Received:2009-09-15 Online:2010-02-25 Published:2010-04-20

摘要/Abstract

摘要： 选用5年生甘肃贝母种株,在盛花期挂牌标记开花一致的植株,从开花后第5天开始测定籽粒千粒鲜、干重和含水量,对种子灌浆特性进行研究,旨在为其种子标准化采收提供理论和技术依据。结果表明,甘肃贝母种子千粒鲜重在开花后66d达到最大,随后迅速下降到接近干重的水平。籽粒干重变化的趋势呈“S”型曲线,符合Logistic方程,快增期在花后41～66d,至花后97d灌浆结束。灌浆速率呈“快-慢-快-慢”规律,因降水出现1次低谷,籽粒脱水速率随灌浆进程而加快,含水量持续下降,籽粒含水量下降最快的时期为灌浆高峰结束期。籽粒干重与脱水速率和灌浆持续期均呈极显著正相关,而与籽粒含水量呈极显著负相关。甘肃贝母种子灌浆速率受天气的影响大,籽粒脱水加快、干重趋于稳定是种子成熟的标志,采收期应在花后77～82d(7月底-8月初),种果尚未开裂,种子含水率在40%以下,茎杆尚未完全枯黄时为最佳。

Abstract: Cards were hung as a flower sign during blossom in lustrum Fritillaria przewalskii plants cultivated in Gannan, Gansu. The grain fresh weight, dry weight and water content in capsules of the plants were measured in period time after 5 d following the day. The grain filling characteristics were studied in order to provide theoretic basis for its seed standard production. The results showed that the 1000-grain fresh weight increased and got at the highest value on the 66th day following blossom, and then rapidly decreased to the level near its dry weight, which fitted to Logistic equation with a rapid increase during 41－66 d after blossom and finished at the end of 97 d. The grain filling rate showed ‘fast-slow-fast-slow’ pattern and went to bottom one time because of raining. The grains dehydrated faster and faster with filling duration elongation and the water content decreased to the end. The fastest decreased time in water content means the finish time of the grain maximum filling. The seed dry weight were positively and significantly related with the grain dehydration rate and the filling duration while negatively and significantly related with water concentration. All above revealed that the seed filling is affected by weather. The fast dehydrating rate and the stability of dry weight in seeds are the important indicates for seed maturation. The best harvest time should be during 77-82 d following blossom (at the end of July or beginning of August), during which the water content in the seeds should be lower than 40% and the seed capsules are not dissilient with still non-perishing stems.

中图分类号:

郭凤霞,常彦莉,林玉红,王三喜,朱君,陈垣. 甘肃贝母种子灌浆特性研究[J]. 草业学报, 2010, 19(2): 97-102.

GUO Feng-xia,CHANG Yan-li,LIN Yu-hong,WANG San-xi,ZHU Jun,CHEN Yuan. Studies on grain filling characteristics of Fritillaria przewalskii[J]. Acta Prataculturae Sinica, 2010, 19(2): 97-102.

参考文献

[1] 杨曦亮, 张勇慧, 阮汉利, 等. 中药贝母的本草考证[J]. 亚太传统医药, 2006, (7): 69-72.
[2] 国家药典委员会. 中华人民共和国药典(一部)[S]. 北京:化学工业出版社, 2005.
[3] 王曙, 徐小平, 李涛. 川贝母与其他贝母类药材总生物碱和总皂苷的含量测定与比较[J]. 中国中药杂志, 2002, 27(5): 342-344.
[4] Li H J, Jiang Y, Li P. Characterizing distribution of steroidal alkaloids in Fritillaria spp. and related compound formulas by liquid chromatography-mass spectrometry combined with hierarchial cluster analysis[J]. Journal of Chromatography A, 2009, 1216(11): 2142-2149.
[5] Lin G, Li P, Li S L, et al. Chromatographic analysis of Fritillaria isosteroidal alkaloids, the active ingredients of Beimu, the antitussive traditional Chinese medicinal herb[J]. Journal of Chromatography A, 2001, 935(1-2): 321-338.
[6] 王天志, 杜蕾蕾, 王曙. 川贝母的研究进展[J]. 华西药学杂志, 2001, 16(3): 200-203.
[7] 金兰, 罗桂花. 低温解除川贝母种子休眠过程中酯酶和过氧化物酶同工酶的变化[J]. 青海师范大学学报(自然科学版), 2007, (4): 57-59.
[8] 石有太, 陈垣, 郭凤霞, 等. 掌叶大黄种子灌浆动态及其发芽特性研究[J]. 草业学报, 2009, 18(3): 178-183.
[9] 石有太, 陈垣, 郭凤霞, 等. 掌叶大黄籽粒营养物质积累动态及其发芽特性研究[J]. 中国中药杂志, 2009, 34(15): 1979-1983.
[10] 赵洋, 陈垣, 郭凤霞, 等. 三年生当归不同节位花穗籽粒灌浆特性研究[J]. 中药材, 2009, 32(6): 837-840.
[11] 明道绪. 田间试验与统计分析[M]. 北京: 科学出版社, 2005.
[12] 李德荣, 舒俭民, 程建峰, 等. 氮磷钾配施对百喜草干物质积累及其动态变化的影响[J]. 草业学报, 2004, 13(5): 60-65.
[13] 武得礼, 葛文华, 何世炜. 甘肃景泰灌区玉米收籽与收刈青贮临界适期的研究[J]. 草业学报, 1999, 8(2): 65-70.
[14] 蔡庆生. 小麦籽粒生长各阶段干物质积累量与粒重的关系[J]. 南京农业大学学报, 1993, 16(1): 27-32.
[15] Mou B, Kronstad W E. Duration and rate of grain filling in selected winter wheat populations: I. Inheritance[J]. Crop Science, 1994, 34(4): 833-837.
[16] 章履孝, 颜伟. 玉米粒重、灌浆持续期、灌浆速率的遗传特性及其关系研究[J]. 江苏农业学报, 1997, 13(4): 211-214.
[17] 李世清, 邵明安, 李紫燕, 等. 小麦籽粒灌浆特征及影响因素的研究进展[J]. 西北植物学报, 2003, 23(11): 2031-2039.
[18] 孙月轩, 姜先梅, 张作木, 等. 夏玉米灌浆与温度、籽粒含水率关系的初步探讨[J]. 玉米科学, 1994, 2(1): 54-59.
[19] 尚占环, 徐鹏彬, 任国华, 等. 土壤种子库研究综述-植被系统中的作用及功能[J]. 草业学报, 2009, 18(2): 175-183.