狼尾草属牧草rDNA的ITS序列分析

草业学报 ›› 2010, Vol. 19 ›› Issue (4): 135-141.

狼尾草属牧草rDNA的ITS序列分析

陈志彤^1,2,黄勤楼²,潘伟彬^2,3,黄毅斌^1,2*

1.福建农林大学生命科学学院,福建福州350002;
2.福建省农业科学院农业生态研究所福建省山地草业工程技术
研究中心,福建福州350013;
3.闽西职业技术学院资源工程系,福建龙岩364021

收稿日期:2009-06-22 出版日期:2010-08-20 发布日期:2010-08-20
作者简介:陈志彤(1979-),男,福建安溪人,助理研究员,在读博士。E-mailcrczt@163.com
基金资助:
国家科技支撑计划项目(2008BADB3B08),农业部“引进国际先进农业科学技术”项目[2006-G8(4)-20-4],福建省科技厅重点项目(2008N0018)和福建省科技厅公益型专项资助。

Sequence analysis of the rDNA ITS region of Pennisetum species (Poaceae)

CHEN Zhi-tong^1,2, HUANG Qin-lou², PAN Wei-bin^2,3, HUANG Yi-bin^1,2

1.College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China;

2.Agricultural Ecology Research Institute, Fujian Academy of Agricultural Sciences/Fujian
Engineering and Technology Research Center for Hilly Prataculture, Fuzhou 350013,
China;
3.Minxi Vocational and Technical College, Resource Engineering
Department, Longyan 364021, China

Received:2009-06-22 Online:2010-08-20 Published:2010-08-20

摘要/Abstract

摘要：

对来自福建、江苏、海南等15份狼尾草属牧草的5.8SrDNA、ITS1及ITS2片段进行克隆及序列分析,并登录于GenBank数据库,采用DNAMAN、CLUSTALX、MEGA等软件分析其遗传关系聚类图。结果显示,克隆的目的片段长度为573～586bp,聚类结果总体能较好地反应狼尾草属牧草之间的遗传距离,其中杂交狼尾草和细茎杂交狼尾草可能存在着同种异名的现象。

Abstract:

The internal transcribed spacer (ITS) regions of nuclear ribosomal DNA from 15 species of Pennisetum (Poaceae) from areas such as Fujian, Jiangsu, Hainan were sequenced and the sequences submitted to the Genbank database. The phylogenetic relationship of the 15 species were studied by CLUSTAL X and MEGA software. Their length of nrDNA ITS region ranged from 573 to 586 bp. P. americanum×P. purpureum and P. americanum×P. purpureum cv. slimstem may be the same variety with different names. The phylogram tree indicated that sequence analysis of nrDNA ITS regions is a good technique for studying the genetic diversity of Pennisetum.

中图分类号:

陈志彤,黄勤楼,潘伟彬,黄毅斌. 狼尾草属牧草rDNA的ITS序列分析[J]. 草业学报, 2010, 19(4): 135-141.

CHEN Zhi-tong, HUANG Qin-lou, PAN Wei-bin, HUANG Yi-bin. Sequence analysis of the rDNA ITS region of Pennisetum species (Poaceae)[J]. Acta Prataculturae Sinica, 2010, 19(4): 135-141.

参考文献

[1] 林洁荣, 刘建昌, 苏水金. 福建南亚热带狼尾草属牧草品比试验[J]. 福建农林大学学报(自然科学版), 2003, 32(1): 110-112.
[2] 王栋. 牧草学各论(新1版)[M]. 任继周, 王槐三, 卢得仁, 修订. 南京: 江苏科学技术出版社, 1989: 121.
[3] 白淑娟, 丁成龙, 顾洪如, 等. 美洲狼尾草资源的鉴定与评价[J]. 国外农学——杂粮作物, 1997, (4): 26-29.
[4] 韦文科. 优质牧草——杂交狼尾草栽培与利用[J]. 农家之友, 2003, (21): 25.
[5] 刘忠, 汪小全, 陈之端, 等. 五味子科的系统发育: 核糖体DNA ITS区序列证据[J]. 植物学报, 2000, 42: 758-761.
[6] 汪小全, 李振宇. rDNA片段的序列分析在苦苣苔亚科系统学研究中的应用[J]. 植物分类学报, 1998, 36(5): 403-410.
[7] Kong H Z, Chen Z D. Phylogeny in chloranthus swartz (Chloranthaceae)inferred from sequence analysis of nrDNA ITS region[J]. Acta Botanica Sinica, 2000, 42: 762-764.
[8] Hsiao C, Chatterton N J, Asay K H. Phylogenetic relationships of the mongenomic species of wheat tribe, Triticeae(poaceae), inferred from nuclear rDNA(ITS) sequences[J]. Theoretical and Applied Genetics, 1995, 90: 389-398.
[9] Baldwin B G, Sanderson M J, Potrer J M, et al. The ITS region of nuclear ribosomal DNA: A valuable source of evidence on angiosperm phylogeny[J]. Annals of the Missouri Botanical Garden, 1995, 82: 247-277.
[10] Schaal B A, Learn G H. Ribosomal DNA variation within and among plant populations[J]. Annals of the Missouri Botanical Garden, 1998, 75: 1207-1216.
[11] Soltis P S, Kuzoff R K. ITRS sequence variation within and among populations of Lomatium grayi and L. laevigatum[J]. Molecular Phylogenetics and Evolution, 1993, 2: 166-170.
[12] 李大勇, 茹岩岩, 张学勇. 中间偃麦草(Thinopyrum intermedium)18S-5.8S-26S rDNA位点在染色体上的分布及对其核ITS序列异质性的分析[J]. 植物学报(英文版), 2004, 46(10): 1234-1241.
[13] 张利, 周永红, 丁春邦, 等. 基于ITS序列分析仲彬草属植物的亲缘关系[J]. 西北植物学报, 2007, 27(3): 490-496.
[14] 施苏华, 王建波. 山羊草属二倍体物种核rDNA ITS区序列及其系统发育关系分析[J]. 植物学报(英文版), 2000, 42(5): 507-511.
[15] 陈志彤, 黄毅斌, 应朝阳, 等. RAPD技术在狼尾草属牧草上的应用[J]. 热带农业科学, 2008, 28(4): 7-10.
[16] Martel E, Poncet V, Lamy F, et al. Chromosone evolution of Pennisetum species(Poaceae): Implications of ITS phylogeny[J]. Plant Systematics and Evolution, 2004, 249: 139-149.
[17] 陈志彤, 陈坚, 郑伟文. 泽泻18S-26S rRNA及其ITS片断的克隆及序列分析[J]. 闽西职业大学学报, 2004, 6(2): 111-115.
[18] 刘春卉, 瞿伟菁, 张雯, 等. 药用真菌金耳的rDNA ITS序列分析与鉴别[J]. 天然产物研究与开发, 2007, 19(2): 216-220.
[19] 詹秋文, 李杰勤, 汪保华, 等. 42份高梁与苏丹草及其2个杂交种DNA指纹图谱的构建[J]. 草业学报, 2008, 17(6): 85-92.
[20] 薛丹丹, 郭海林, 郑轶琦, 等. 结缕草属植物杂交后代杂种真实性鉴定——SRAP分子标记[J]. 草业学报, 2009, 18(1): 72-79.
[21] 刘欢, 赵桂琴, 耿小丽, 等. 燕麦AFLP反应体系的建立与优化[J]. 草业科学, 2008, 25(2): 84-89.
[22] 唐燕琼, 胡新文, 郭建春, 等. 柱花草种质遗传多样性的ISSR分析[J]. 草业学报, 2009, 18(1): 57-64.
[23] 严学兵, 王堃, 周禾, 等. 不同来源SSR标记在我国披碱草属植物的通用性和效率评价[J]. 草业学报, 2008, 17(6): 112-120.
[24] 王建波, 张文驹,陈家宽. 核rDNA的ITS序列在被子植物系统与进化研究中的应用[J]. 植物分类学报, 1999, 37(4): 407-414.
[25] Koh N, Chung S W, Goro K, et al. Phylogenetic systematics of the monotypic genus Hayataella(Rubiaceae) endemic to Taiwan[J]. Journal of Plant Research, 2006, 119: 657-661.
[26] Nobuo K, Daiki M, Akira N, et al. Attaining inter-subgeneric hybrids in fragrant azalea breeding and the inheritance of organelle DNA[J]. Euphytica, 2008, 159(1): 67-72.
[27] Touloumenidou T, Bakker F T, Albers F. The phylogeny of Monsonia L. (Geraniaceae)[J]. Plant Systematics and Evolution, 2007, 264: 1-14.