高粱棕色中脉基因bmr-6的遗传分析和SSR标记定位

草业学报 ›› 2010, Vol. 19 ›› Issue (5): 273-277.

高粱棕色中脉基因bmr-6的遗传分析和SSR标记定位

李杰勤,王丽华,詹秋文^*,范军成

安徽科技学院植物科学学院,安徽凤阳 233100

收稿日期:2009-11-25 出版日期:2010-05-25 发布日期:2010-10-20
作者简介:李杰勤(1980-),男,四川宜宾人,硕士。E-mail:wlhljq@163.com
基金资助:
科技部农业科技成果转化资金项目(No2008GB2C300125),国家自然科学基金(No31071470),安徽省“115”产业创新团队资助(No皖人才办[2009]2号),国家科技支撑计划项目(No2008BADB3B10),安徽省高校学科拔尖人才基金(No教秘人[2005]79号)和安徽科技学院稳定人才专项( NoZRC2009236)资助。

Genetic analysis and mapping with SSR markers of the brown midrib gene bmr-6 in Sorghum bicolor

LI Jie-qin, WANG Li-hua, ZHAN Qiu-wen, FAN Jun-cheng

College of plant sicence and technology, Anhui Science and Technology
University, Fengyang 233100, China

Received:2009-11-25 Online:2010-05-25 Published:2010-10-20

摘要/Abstract

摘要： 高粱棕色突变体的叶片中脉是棕色的,此类突变体能使家畜难以消化的木质素含量降低40%～60%,从而大幅度提高了家畜对高粱秸秆的消化率。利用高粱棕色中脉材料N592(bmr-6类型)和白色中脉(Sa)进行杂交和自交,构建F₂分离群体,F₁表现为白色中脉,F₂白棕分离比符合3∶1,说明棕色中脉基因受一对等位基因控制,表现为隐性遗传。用已知定位到连锁群上的微卫星标记对棕色中脉基因进行了连锁分析,发现1个与棕色中脉(bmr-6)基因连锁的微卫星标记,连锁距离为4.2cM,初步将该基因定位于第7连锁群。

Abstract: The mutants, which produced brown leaf midribs, reduced the content of lignin by 40% to 60% compared with normal varieties. This low lignin content largely promoted the rate of digestion in livestock. The F₂ population was constructed from the hybrid of N592 (bmr-6) and Sa (white midrib) to map the bmr-6 gene. The ratio of white and brown midribs was 3 to 1 in the F₂ population. Therefore, the bmr-6 gene is a single recessive gene. Using 171 SSR markers and F₂ recessive population, one SSR (simple sequence repeats) marker (txp295) that linked with the bmr-6 gene was detected and the genetic distance was 4.2 cM. In addition, the bmr-6 gene was mapped to the seventh linkage group of sorghum because the txp295 marker was located on this linkage group.

中图分类号:

S514.03
Q943

李杰勤,王丽华,詹秋文,范军成. 高粱棕色中脉基因bmr-6的遗传分析和SSR标记定位[J]. 草业学报, 2010, 19(5): 273-277.

LI Jie-qin, WANG Li-hua, ZHAN Qiu-wen, FAN Jun-cheng. Genetic analysis and mapping with SSR markers of the brown midrib gene bmr-6 in Sorghum bicolor[J]. Acta Prataculturae Sinica, 2010, 19(5): 273-277.

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