利用生理生化参数评价水葫芦沼液浸种可行性初步研究

草业学报 ›› 2010, Vol. 19 ›› Issue (5): 51-56.

利用生理生化参数评价水葫芦沼液浸种可行性初步研究

薛延丰^1,2,3, 石志琦^1,2,3, 严少华^4*, 郑建初^4*, 常志州^4*

1.江苏省农业科学院食品质量安全检测研究所, 江苏南京 210014;
2. 江苏省食品质量安全重点实验室-省部
共建国家重点实验室培育基地, 江苏南京 210014;
3.农业部食品安全监控重点开放实验室,
江苏南京 210014;
4.江苏省农业科学院农业资源与环境研究所, 江苏南京210014

收稿日期:2009-10-23 出版日期:2010-05-25 发布日期:2010-10-20
作者简介:薛延丰(1978-),男,河南孟州人,博士。E-mail: xueyanfeng@jaas.ac.cn
基金资助:
国家科技支撑项目(2009BAC63B02)资助。

A preliminary study of assessing the feasibility of using physiological and biochemical parameters to assess water hyacinth biogas slurry for soaking seeds to improve germination characteristics

XUE Yan-feng^1,2,3, SHI Zhi-qi^1,2,3, YAN Shao-hua⁴, ZHENG Jian-chu⁴, CHANG Zhi-zhou⁴

1.Institute of Food Quality and Safety, Jiangsu Academy of Agricultural Sciences, Nanjing 210014,
China;
2.Key Lab of Food Quality and Safety of Jiangsu Province—State Key Laboratory Breeding
Base, Nanjing 210014, China;
3.Key Lab of Agro-Food Safety and Quality, Ministry of
Agriculture, Nanjing 210014, China;
4.Institute of Agricultural Resources
and Environment, Jiangsu Academy of Agricultural Sciences,
Nanjing 210014, China

Received:2009-10-23 Online:2010-05-25 Published:2010-10-20

摘要/Abstract

摘要： 以青菜为材料,研究了不同浓度水葫芦沼液进行青菜种子浸种后对种子发芽、生长、叶绿素含量及抗氧化酶活性的影响。结果表明,沼液浸种可以提高种子的发芽势和发芽率,但与对照相比差异不显著;发芽指数和活力指数随着沼液处理浓度的增加与对照相比显著增加;随着处理沼液施用浓度的增加,青菜的根长逐渐增加,Z1和Z4处理株高与对照相比,虽然有所增加,但与对照差异不显著;株高、鲜重和干重的变化趋势相似,随着沼液处理浓度的增加,株高、鲜重和干重的增加幅度也随之增大,当使用全沼液进行处理后虽然有所降低,但与对照相比显著增加。青菜体内的叶绿素含量、抗氧化酶SOD、POD和CAT活性与对照相比显著增加,当使用全沼液处理后,叶绿素含量与抗氧化酶活性虽然有所降低,但与对照相比显著增加。因此,生理生化参数可作为评判水葫芦沼液浸种可行性的指标之一。

Abstract: The seeds of Chinese cabbage (Brassica rapa) were soaked in a range of concentrations of biogas slurry from water hyacinth (Eichhornia crassipes) fermentation. The effects of the slurry on seed germination, growth, chlorophyll content and changes of antioxidant enzymes in Chinese cabbages were studied. The germination energy and germination percentage increased with an increase of biogas slurry concentration, but the differences were not significant compared with the controls. The germination index and vigor index increased markedly with an increased concentration of biogas slurry. With the exception of Z1 and Z4, the root lengths increased significantly compared with the controls as did shoot height, fresh weight and dry weight: All these parameters increased markedly with an increase of biogas slurry concentration. Although the parameters decreased in the treatment of purified biogas slurry, they were significantly different from the control. The trend of chlorophyll content and antioxidant enzyme activities changed as for the growth parameters. Therefore, the physiological and biochemical parameters could be used as a simple index for quickly assessing the feasibility of soaking seeds in water hyacinth biogas slurry to improve germination.

中图分类号:

薛延丰, 石志琦, 严少华, 郑建初, 常志州. 利用生理生化参数评价水葫芦沼液浸种可行性初步研究[J]. 草业学报, 2010, 19(5): 51-56.

XUE Yan-feng, SHI Zhi-qi, YAN Shao-hua, ZHENG Jian-chu, CHANG Zhi-zhou. A preliminary study of assessing the feasibility of using physiological and biochemical parameters to assess water hyacinth biogas slurry for soaking seeds to improve germination characteristics[J]. Acta Prataculturae Sinica, 2010, 19(5): 51-56.

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