NaCl胁迫对芨芨草苗期脯氨酸代谢的影响

摘要/Abstract

摘要： 以芨芨草为试验材料,在不同浓度NaCl胁迫下,对芨芨草叶片与根系脯氨酸(proline,Pro)、鸟氨酸δ-氨基转移酶(ornithine-oxo-acidtransaminase,δ-OAT)、吡咯啉-5-羧酸合成酶(pyrroline-5-carboxylatesynthetase,P5CS)、脯氨酸脱氢酶(prolinedehydragenase,ProDH)进行测定。结果表明,NaCl胁迫下,芨芨草Pro的积累主要以谷氨酸途径为主;随NaCl浓度的增加,芨芨草叶片和根系Pro含量逐渐增加,500mmol/LNaCl时达最大,分别为585.77和303.36μg/gFW;δ-OAT活性呈降低趋势,均显著低于对照(P<0.05);在同一浓度胁迫下,芨芨草根系P5CS活性均显著高于叶片(P<0.05),300mmol/LNaCl时,芨芨草叶片与根系P5CS活性均达到最大,分别为7.44和20.81U/mg蛋白;叶片ProDH活性高于对照,最大为对照的1.7倍,根系ProDH活性变化不明显。

Abstract: Under varying levels of NaCl stress, the proline(Pro), pyrroline-5-carboxylate synthetase(P5CS), ornithine-oxo-acid transaminase(δ-OAT), and proline dehydragenase(ProDH) among leaves and roots of Achnatherum splendens were determined. The results showed that the proline metabolism synthesis path-way of the A. splendens seedling is mainly on the pathway of Glutamate.The proline content increased with the increase of NaCl,and the proline content in leaves and in roots were the highest that could be up to 585.77 and 303.36 μg/g FW respectively at the concentration of 500 mmol/L NaCl. The activity of δ-OAT was significantly lower than that in control(P<0.05), and declined with the increasing of the NaCl concentration. The activity of P5CS from the roots of Achnatherum splendens was significantly higher than leaves under the same concentration(P<0.05). At the concentration of 300 mmol/L NaCl,the P5CS activity in leaves and roots reached the 7.44 and 20.81 U/mg Protein respectively. The activity of ProDH in leaves were higher than in control, and could be up to 1.7 times than the control, but didn't change significantly in roots.

中图分类号:

董秋丽,夏方山,董宽虎. NaCl胁迫对芨芨草苗期脯氨酸代谢的影响[J]. 草业学报, 2010, 19(5): 71-76.

DONG Qiu-li, XIA Fang-shan, DONG Kuan-hu. Effects of NaCl stress on proline metabolism of Achnatherum splendens seedling[J]. Acta Prataculturae Sinica, 2010, 19(5): 71-76.

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