太子参连作障碍蛋白差异表达分析

摘要/Abstract

摘要： 为探明太子参连作障碍分子机理,本研究采用差异蛋白质组学方法研究了不同种植制度下(连作、轮作)太子参叶片蛋白质的差异表达。结果表明,不同种植制度下太子参叶片中共有27个差异表达蛋白质点,经生物信息学查询到24个蛋白质。差异蛋白质功能分析结果表明,连作导致太子参叶片中与植物衰老或病害相关的5个蛋白表达量全部上调;与植物抗性相关的5个蛋白(磷脂氢谷胱苷肽过氧化物酶、植物性丝氨酸-苏氨酸蛋白激酶、钙依赖蛋白激酶、查尔酮合成酶和硫氧还相关蛋白)表达量上调,4个蛋白(细胞壁富含甘氨酸蛋白、SKP1同源蛋白、锌指蛋白和成熟酶K)表达量下调;与光合作用相关的2个蛋白(Rubisco大亚基和细胞色素b₆)表达量下调,2个蛋白(核酮糖二磷酸碳酸酵素小链c和细胞色素b₆/f复合体亚基VI)表达量上调;与能量代谢相关的蔗糖-UDP葡萄糖基转移酶和与细胞分裂相关的NF-Y5表达量均下调,而蛋白酶1型β亚基、ATP合成酶β亚基、细胞分裂素组氨酸磷光体转移蛋白4和NAD依赖-3-磷酸甘油醛脱氢酶表达量均上调。这说明太子参连作导致叶片致病性或衰老相关蛋白表达量上调,是太子参连作病害频发和提前退黄的主要原因,同时连作导致抗性相关蛋白以及能量代谢和细胞分裂相关蛋白紊乱(或降低或上调),是太子参连作障碍的结果在蛋白质水平的表现,连作还导致重要的光合相关蛋白表达量下调,是连作太子参光合作用能力下降的分子基础。

Abstract: The differential proteomics method was used to analyze the differential expression of proteins in Pseudostellaria heterophylla leaves to reveal the molecular mechanism of P. heterophylla replanting disease in a P. heterophylla-Oryza sativa-P. heterophylla crop rotation or in P. heterophylla continuous cropping. There were 27 differential protein spots in the 2-D gelatin from the P. heterophylla leaves in both the crop rotation and the continuous cropping systems. Bioinformatics searches successfully identified 24 of the 27 protein spots. Senescence-associated similar proteins, the Ras-related protein, the senescence-related receptor-like kinases, the PR-proteins and the Ras-related protein RHN1 were all up-regulated. All of these proteins are related to plant senescence or disease. The phospholipid hydroperoxide glutathione peroxidase, plant-type serine-threonine protein kinase, calcium-dependent protein kinase, chalcone synthase, and thioredoxin-related proteins were all up-regulated, while the cell wall glycine-rich protein, the homologue to SKP1, the zinc finger protein, and the maturase K were all down-regulated. These proteins are related to plant resistance. Both the ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit and the cytochrome b₆ were down-regulated, and the ribulose bisphosphate carboxylase small chain c and the cytochrome b₆/f complex subunit VI were up-regulated. These proteins are related to photosynthesis. The sucrose-UDP glucosyltransferase and the NF-YB5 were down-regulated, and the proteasome subunit beta type 1, the synthase beta subunit, the cytokinin histidine phosphotransfer protein 4 and the NAD-dependent glyceraldehyde-3-phosphate dehydrogenase were all up-regulated. These proteins are related to plant metabolism of energy or cell division. It suggested that in P. heterophylla replanting disease, up-regulated expression of proteins associated with senescence or disease results in disturbed expressions of proteins related to plant resistance or to energy metabolism or cell division. The continuous planting of P. heterophylla resulted in down-regulated expression of the important photosynthesis related proteins, explaining the molecular basis for the declining photosynthesis rate in replanted P. heterophylla.

中图分类号:

林茂兹,张志兴,林争春,尤垂怀,曾令杰,林文雄. 太子参连作障碍蛋白差异表达分析[J]. 草业学报, 2010, 19(6): 197-207.

LIN Mao-zi, ZHANG Zhi-xing, LIN Zheng-chun, YOU Chui-huai,
ZENG Ling-jie, LIN Wen-xiong. Analysis of differential expression of proteins in replanting disease of Pseudostellaria heterophylla[J]. Acta Prataculturae Sinica, 2010, 19(6): 197-207.

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