中国高羊茅种质资源遗传多样性的RAPD分析

草业学报 ›› 2010, Vol. 19 ›› Issue (6): 208-214.

中国高羊茅种质资源遗传多样性的RAPD分析

李惠英,娄燕宏,胡涛,傅金民^*

中国科学院武汉植物园植物种质创新与特色农业重点实验室,湖北武汉 430074

收稿日期:2010-01-12 出版日期:2010-06-25 发布日期:2010-12-20
作者简介:李惠英(1977-),女,河南安阳人,助理研究员,博士。
基金资助:
中国科学院知识创新项目资助。

Genetic diversity analysis of Chinese natural tall fescue (Festuca arundinacea) germplasms by RAPD

LI Hui-ying, LOU Yan-hong, HU Tao, FU Jin-min

Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture,
Wuhan Botanical Garden, CAS, Wuhan 430074, China

Received:2010-01-12 Online:2010-06-25 Published:2010-12-20

摘要/Abstract

摘要： 高羊茅是广泛应用的冷季型草坪草,但有关我国高羊茅种质资源遗传多样性的研究十分匮乏。本研究利用RAPD分子标记技术,对采自我国云南、贵州、青海、湖北等地的26份逸生高羊茅种质资源进行了遗传多样性分析。试验筛选出引物20条,共扩增出179个条带,其中177个为多态性带,平均每个引物扩增出多态性带8.85条,多态性条带比率(PPB)为98.88%。材料间遗传相似系数为0.419~0.966,说明我国逸生高羊茅种质资源具有丰富的遗传多样性。根据聚类分析结果,可将26份中国本土高羊茅材料分为三大类,其中来自云南省的高羊茅基本聚在同一类,来自其余省份的高羊茅种质资源的聚类结果与其地域分布没有明显联系。

Abstract: Tall fescue (Festuca arundinacea) is a cool season turfgrass widely used in China. The genetic variation of wild tall fescue germplams was investigated. Twenty six tall fescue ecotypes collected from Yunnan, Guizhou, Qinghai and Hubei provinces were assessed for genetic diversity analysis by random amplified polymorphic DNA (RAPD). Twenty of the 41 arbitrary primers totally amplified 179 DNA bands, an average of 8.95 DNA bands per primer. The polymorphic bands (177) accounted for 98.88% of total DNA bands. Jaccard’s genetic similarity coefficient of these tall fescue ecotypes ranged from 0.419 to 0.966, suggesting a significant variation among these ecotypes. An UPGMA dendrogram was established for 26 tall fescue ecotypes based on the genetic similarity matrix. The 26 ecotypes could be divided into three categories based on cluster analysis. The tall fescue ecotypes collected from Yunnan province located in one group. However, tall fescues that came from the other three provinces were not always closely related to their ecological environment.

中图分类号:

李惠英,娄燕宏,胡涛,傅金民. 中国高羊茅种质资源遗传多样性的RAPD分析[J]. 草业学报, 2010, 19(6): 208-214.

LI Hui-ying, LOU Yan-hong, HU Tao, FU Jin-min. Genetic diversity analysis of Chinese natural tall fescue (Festuca arundinacea) germplasms by RAPD[J]. Acta Prataculturae Sinica, 2010, 19(6): 208-214.

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