麦角生物碱在醉马草内生真菌共生体中的空间分布

草业学报 ›› 2010, Vol. 19 ›› Issue (6): 215-221.

麦角生物碱在醉马草内生真菌共生体中的空间分布

代乐英,黄玺,李春杰^*,南志标

兰州大学草地农业科技学院,甘肃兰州 730000

收稿日期:2009-07-24 出版日期:2010-06-25 发布日期:2010-12-20
作者简介:乐英(1983-),女,甘肃榆中人,在读硕士。E-mail:daileying@163.com
基金资助:
国家973项目(2007CB108902),国家科技支持项目(2008BADB3B05),教育部新世纪优秀人才支持计划项目(NCET-08-0256)和国家自然科学基金项目(30771531,30070546)资助。

Spatial variation of ergot alkaloids in drunken horse grass infected by Neotyphodium gansuense

DAI Le-ying, HUANG Xi, LI Chun-jie, NAN Zhi-biao

College of Pastoral Agricultural Science and Technology, Lanzhou University, Lanzhou 730020, China

Received:2009-07-24 Online:2010-06-25 Published:2010-12-20

摘要/Abstract

摘要： 本研究检测了2007年9月采自新疆哈密(HM)、青海铁卜加(TBJ)、甘肃肃南(SN)、甘肃天祝(TZ)、甘肃夏河(XH)5个不同地理种群的醉马草内生真菌共生体的麦角生物碱含量,结果表明,醉马草种群内不同器官中麦角新碱及麦角酰胺含量均不同,且麦角新碱的含量均显著高于麦角酰胺的含量(P<0.05)。5个种群间麦角酰胺、麦角新碱及总生物碱的含量除叶鞘外,均以HM最高,分别为53.656,117.784和160.816mg/kg干重。相关性分析表明,植株叶片、茎秆和果穗麦角酰胺、麦角新碱及总生物碱的含量均与种群生长的年均温度呈正相关关系。

Abstract: Ergot alkaloids were detrected in drunken horse grass (Achnatherum inebrians)-Neotyphodium gansuense endophyte symbionts taken from 5 different geographical sites: Hami in Xinjiang (HM), Tiebujia in Qinghai (TBJ), Sunan (SN), Tianzhu (TZ) and Xiahe (XH) in Gansu in September 2007. Different ergine and ergonovine contents were found in different organs of drunken horse grass and the ergonovine contents were significantly greater than those of ergine (P<0.05). The ergine, ergonovine and total alkaloid contents (53.656, 117.784 and 160.816 mg/kg dry matter, respectively) were highest in plants from HM except in the leaf sheaths. Correlation analyses indicated that ergine, ergonovine and total alkaloid contents of leaf blades, stems and heads of plants were positively related to the annual average temperature.

中图分类号:

代乐英,黄玺,李春杰,南志标. 麦角生物碱在醉马草内生真菌共生体中的空间分布[J]. 草业学报, 2010, 19(6): 215-221.

DAI Le-ying, HUANG Xi, LI Chun-jie, NAN Zhi-biao. Spatial variation of ergot alkaloids in drunken horse grass infected by Neotyphodium gansuense[J]. Acta Prataculturae Sinica, 2010, 19(6): 215-221.

参考文献

[1] 史志诚. 中国草地重要有毒植物[M]. 北京: 中国农业出版社, 1997: 166-176.
[2] 马松江. 青海海西草原有毒植物及其经济价值[J]. 草业科学, 2008, 25(5): 98-103.
[3] 高嘉卉, 南志标. 禾草内生真菌生物碱的研究进展[J]. 生态学报, 2007, 27(6): 2531-2546.
[4] 南志标, 李春杰. 禾草-内生真菌共生体在草地农业系统中的作用[J]. 生态学报, 2004, 24(3): 605-616.
[5] Miles C O, Lane G, Piper E L, et al. High levels of ergonovine and lysergic acid amide in toxic Achnatherum inebrians accompany infection by an Acremonium-like endophytic fungus[J]. Journal of Agriculture & Food Chemistry, 1996, 44(5): 1285-1290.
[6] Porter J K. Chemical constituents of grass endophytes[A]. In: Bacon W C, White J F Jr. Biotechnology of Fndophytic Fungi of Grasses[C]. Boca Raton: CRC Press, 1994: 103-124.
[7] Clark E M, White J F, Patterson R M. Improved histochemical techniques for the detection of Acremonium coenophialum in tall fescue and methods of in vitro culture of the fungus[J]. Microbiology Methods, 1983, 1: 149-155.
[8] 李飞, 李春杰. 内生真菌对禾草类植物抗旱性的影响[J]. 草业科学, 2006, 23(3): 57-62.
[9] Li C J, Gao J H, Nan Z B. Interactions of Neotyphodium gansuense, Achnatherum inebrians and plant-pathogenic fungi[M]. Mycological Research, 2007, 111(10): 1220-1227.
[10] 张兴旭, 陈娜, 李春杰, 等. 禾谷缢管蚜与内生真菌互作对醉马草幼苗的生理影响[J]. 草地学报, 2008, 16(3): 239-244.
[11] 陈娜. 醉马草遗传多样性及内生真菌对其抗寒性影响[D]. 兰州: 兰州大学, 2008.
[12] 缑小媛. 内生真菌对醉马草耐盐性的影响研究[D]. 兰州: 兰州大学, 2007.
[13] Leuchtmann A, Schmidt D, Bush L P. Different levels of protective alkaloids in grasses with stroma-porming and seed-transmitted Epichlo/Neotyphodium endophytes[J]. Journal of Chemical Ecology, 2000, 26(4): 1025-1036.
[14] Caga B, Flieger M, Olovsk J. Concentration of ergot alkaloids in Czech ecotypes of Lolium perenne and Festuca pratensis[J]. Grass and Forage Science, 1999, 54(4): 365-370.
[15] Faeth S H, Bush L P, Sullivan T J. Peramine alkaloid variation in Neotyphodium-infected arizona fescue: Effects of endophyte and host genotype and environment[J]. Journal of Chemical Ecology, 2002, 28(8): 1511-1527.
[16] Hill N S, Bouton J H, Adcock R A, et al. Symbiont regulation and reducing ergot alkaloid concentrattion by breeding endophyte-inffected tall fescue[J]. Journal of Chemical Ecology, 1997, 23(3): 691-701.
[17] Bacon C W. Toxic endophyte-infected tall fescue and range grasses: Historic perspectives[J]. Journal of Animal Science, 1979, 73: 861-870.
[18] Dimenna M E, Mortimer P H, Prestidge R A, et al. Lolitrem B concentrations, counts of Acremonium lolii hyphae, and the incidence of ryegrass staggers in lambs on plots of A. lolii-infected perennial ryegrass[J]. New Zealand Journal of Agricultural Research, 1992, 35: 221-217.
[19] Blankenship J D, Spiering M J, Wilkinson H H, et al. Production of loline alkaloids by the grass endophyte, Neotyphodium uncinatum, in defined media[J]. Phytochemistry, 2001, 58: 395-401.
[20] 高嘉卉. Neotyphodium gansuense离体麦角碱的检测和N. lolii麦角碱基因的测序[D]. 兰州: 兰州大学, 2006.
[21] Malinowski D P, Belesky D P, Hill N S, et al. Influence of phosphorus on the growth and ergot alkaloid content of Neotyphodium coenophialum infected tall fescue (Festuca arundinacea)[J]. Plant and Soil, 1998, 198: 53-61.
[22] Siegel M R, Latch G C, Bush L P, et al. Fungal endophyte-infected grasses: Alkaloid accumulation and aphid response[J]. Journal of Chemical Ecology, 1990, 16: 3301-3315.
[23] Siegel M R, Latch G C M, Johnson M C. Fungal endophytes of grasses[J]. Annual Review of Phytopathology, 1987, 25: 293-315.
[24] Bush L P, Wilkinson H H, Schardl C L. Bioprotective alkaloids of grass-fungal endophyte symbioses[J]. Plant Physiology, 1997, 144: 1-7.
[25] Faeth S H, Gardner D R, Hayes C J, et al. Temporal and spatial variation in alkaloids levels in Achnatherum robustum, a native grass infected with the Neotyphodium[J]. Journal of Chemical Ecology, 2006, 32(2): 307-323.
[26] Li C J, Nan Z B, Christopher S. Levels and temporal variation of ergot alkaloids in endophyte-infected drunken horse grass, Achnatherum inebrians, in China[A]. APS, CPS and MSA Joint Meeting Abstracts[C]. American: Mycological Society of American, 2006: 203-204.
[27] 李春杰. 醉马草-内生真菌共生体生物学与生态学特性的研究[D]. 兰州: 兰州大学, 2005.
[28] Ball O J P, Barker G M, Prestidge R A, et al. Distribution and accumulation of the alkaloid peramine in Neotyphodium lolii-infected perennial ryegrass[J]. Chemical Ecology, 1997, 23: 1419-1434.
[29] Reinholz J, Paul V H. Effect of temperature on the lolitrem B content in Lolium perenne infected by Neotyphodium lolii[A]. The 3rd International Conference on Harmful and Beneficial Microorganisms in Grassland, Pastures and Turf[C]. American: Mycological Society of American, 2000: 57-63.
[30] 唐中华, 于景华, 杨逢建, 等. 植物生物碱代谢生物学研究进展[J]. 植物学通报, 2003, 20(6): 696-702.