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草业学报 ›› 2011, Vol. 20 ›› Issue (1): 148-154.

• 研究论文 • 上一篇    下一篇

贮藏条件和超干燥对塔落岩黄芪种子生理生化特性的影响

陈志宏1,2,谷继承2,李晓芳2,贠旭疆2,高洪文1*   

  1. 1.中国农业科学研究院北京畜牧兽医研究所,北京 100193;
    2.全国畜牧总站,北京 100125
  • 出版日期:2011-02-22 发布日期:2011-02-22
  • 通讯作者: E-mail:gaohongwen2263.net
  • 作者简介:陈志宏(1974-),女,内蒙古土右旗人,畜牧师,在读博士。
  • 基金资助:
    现代化产业体系专项资金和十一五科技支撑(2008BADB3B01)资助。

Effect of ultra-dry treatment on seed storage of Hedysarum laeve

CHEN Zhi-hong1,2, GU Ji-cheng2, LI Xiao-fang2, YUN Xu-jiang2, GAO Hong-wen1   

  1. 1.Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing 100193, China;

    2.National Animal & Husbandry Service, Beijing 100125, China
  • Online:2011-02-22 Published:2011-02-22

摘要: 采用硅胶干燥法将塔落岩黄芪种子含水量由原来的7.900%降至4.645%,3.460%,2.921%和1.829%,用铝箔袋密封后在-4℃、4℃、室温和45℃条件下贮存12个月后测定各项生理生化指标。结果表明,种子含水量在2.921%~4.645%内可以提高塔落岩黄芪种子的耐贮性,增加塔落岩黄芪种子膜透性,增加SOD、POD、CAT活性分别至4.74 U/mg, 0.233 U/mg,2.91 U/(min·g),降低MDA含量至59.72 nmol/L,3.460%为塔落岩黄芪种子超干贮存的适宜含水量。室温适度超干一定程度上可以起到低温未超干的保存效果,因此认为适度超干可以作为塔落岩黄芪种质资源保护的有效措施。

Abstract: The effect of ultra-dry treatment on seed storage of Hedysarum laeve was researched. The seed moisture contents were reduced from 7.900% to 1.829%, 2.921%, 3.460%, or to 4.645% by silica gel, and were then stored at -4℃, at 4℃, at room temperature, or at 45℃. They were tested for physiological and chemical indexes after 12 months. Appropriate ultra-dry treatment enhanced storage effectiveness, increased membrane permeable and activity of SOD, POD, and CAT, but reduced the MDA content of H. laeve seeds. The optimal moisture content for ultra-dry storage was 3.460%. Ultra-drying at room temperature resulted in the same storage effects as treatment at low temperature. Appropriate ultra-dry treatment was therefore an effective conservation measure for H. laeve germplasm resources.

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