美洲南瓜(Cucurbita pepo)种皮发育形态观察及其相关酶活性测定

草业学报 ›› 2011, Vol. 20 ›› Issue (2): 23-30.

美洲南瓜(Cucurbita pepo)种皮发育形态观察及其相关酶活性测定

薛应钰^1,2,师桂英³,徐秉良^1,2*,陈荣贤⁴

1.甘肃农业大学草业学院草业生态系统教育部重点实验室中-美草地畜牧业可持续发展研究中心,甘肃兰州 730070;
2. 甘肃省作物遗传改良与种质创新重点实验室,甘肃兰州 730070;
3.甘肃农业大学农学院,甘肃兰州 730070;
4.甘肃省武威金苹果有限责任公司,甘肃武威 733000

收稿日期:2010-09-16 出版日期:2011-02-25 发布日期:2011-04-20
通讯作者: E-mail:xubl@gsau.edu.cn
作者简介:薛应钰(1978-),男,甘肃镇原人,讲师,在读博士。E-mail:xueyy@gsau.edu.cn
基金资助:
国家自然科学基金项目 (30671267)和甘肃省科技厅中青年基金项目 (3YS051-A25-015)资助。

Studies on morphology of seed coat development and its related enzyme activity assay in Cucurbita pepo

XUE Ying-yu^1,2,SHI Gui-ying³,XU Bing-liang^1,2, CHEN Rong-xian⁴

1.College of Grassland Science, Gansu Agricultural University, Key Laboratory of Grassland Ecosystem, Ministry of Education, Sino-U.S. Centers for Grassland Ecosystem Sustainability, Lanzhou 730070, China;
2.Gansu Key Laboratory of Crop Genetic&Germplasm Enhancement, Lanzhou 730070, China;
3.College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China;
4.Wuwei Golden Apple Co., Ltd., Wuwei 733000, China

Received:2010-09-16 Online:2011-02-25 Published:2011-04-20

摘要/Abstract

摘要： 采用组织整体染色和石蜡切片相结合的方法对美洲南瓜有壳品种04LAg-26-2和无壳品种04LAg-26-28种皮发育形态结构比较观察。结果表明,有壳美洲南瓜种皮是由表皮层、下皮层、厚壁组织层、软组织层和绿色组织层构成,无壳裸仁南瓜的形成主要是授粉后18 d种皮发育过程中厚壁组织中的细胞变形、瓦解,缺少木质素的积累而导致了下皮层、厚壁组织层的缺失;对2个品种的种皮、叶片、叶柄和瓜囊不同组织中木质素合成相关酶活性测定结果表明,无壳品种04LAg-26-28在授粉18 d后,种皮中苯丙氨酸解氨酶(PAL)、肉桂酸-4-羟基化酶(C₄H)、4-香豆酸-辅酶A连接酶(4CL)、肉桂醇脱氢酶(CAD)和过氧化物酶(POD)活性明显降低,而叶片、叶柄和瓜囊中这几种酶活性在授粉18 d 后,呈逐渐增加的趋势。

Abstract: The morphological structure of seed-coat development of two isogenic lines of Cucurbita pepo(normal, 04LAg-26-2 and hull-less, 04LAg-26-28) were comparatively studied through overall staining and paraffin section method. The results showed that the normal seed were composed of five tissue layers: The epidermis, hypodermis, sclerenchyma, parenchyma and chlorenchyma. The formation of hull-less seed was the cells of hypodermis tissues deformed, collapsed and lack of lignin accumulation among the development of seed-coat forming,so that the epidermis, parenchyma and chlorenchyma in the seed-coat were imperfect. The activity of ligin synthesis enzyme in different tissues including testa, leaf, petiole and capsule was tested, the results were as follows: The activity of phenylalanine ammonia-lyase (PAL), cinnamic acid 4-hydroxylase (C₄H), 4-coumarate CoA ligase (4CL), cinnamyl alcohol dehydrogenase (CAD) and peroxidase (POD) in testa of 04LAg-26-28 reduced on 18 days after pollination, but the activity of all enzymes in leaf, petiole and capsule tissues increased significantly.

中图分类号:

薛应钰,师桂英,徐秉良,陈荣贤. 美洲南瓜(Cucurbita pepo)种皮发育形态观察及其相关酶活性测定[J]. 草业学报, 2011, 20(2): 23-30.

XUE Ying-yu,SHI Gui-ying,XU Bing-liang, CHEN Rong-xian. Studies on morphology of seed coat development and its related enzyme activity assay in Cucurbita pepo[J]. Acta Prataculturae Sinica, 2011, 20(2): 23-30.

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