假俭草体细胞抗寒突变体的获得及其SRAP分子鉴定

草业学报 ›› 2011, Vol. 20 ›› Issue (6): 237-244.

假俭草体细胞抗寒突变体的获得及其SRAP分子鉴定

袁学军^1,2,王志勇^1,3,郑轶琦¹,刘建秀^1*,佘建明¹

1.江苏省中科院植物研究所,江苏南京 210014;
2.琼州学院生物科学与技术学院,海南三亚 572000;
3.海南大学农学院,海南儋州 571737

收稿日期:2010-10-19 出版日期:2011-06-25 发布日期:2011-12-20
通讯作者: E-mail:turfunit@yahoo.com.cn
作者简介:袁学军(1967-),男,山东济宁人,副教授,博士。E-mail:yuanxuej@163.com
基金资助:
江苏省科技支撑项目(BE2008403)和江苏省科技厅平台项目(BM2009905)资助。

Acquisition and identification of cold-resistant somatic mutants of centipedegrass

YUAN Xue-jun^1,2, WANG Zhi-yong^1,3, ZHENG Yi-qi¹, LIU Jian-xiu¹, SHE Jian-ming¹

1.Institute of Botany, Jiangsu Province & Chinese Academy of Sciences, Nanjing 210014, China;
2.College of Life Science, Qiongzhou University,Sanya 572000, China;
3.College of Agronomy, Hainan University, Danzhou 571737, China

Received:2010-10-19 Online:2011-06-25 Published:2011-12-20

摘要/Abstract

摘要： 假俭草抗寒性差是限制其广泛应用的主要因素之一。本研究目标是以优良假俭草选系E-126为材料,经低温诱导和筛选获得体细胞抗寒突变体。结果表明,假俭草种子诱导的愈伤组织在继代培养的过程中,经0℃的低温条件下培养26 d,获得了2块存活的愈伤组织,该愈伤组织经过继代增殖后,进行分化、生根和移栽,获得株系1和株系2。苗期外部形态观察结果表明,假俭草低温诱导的株系1、株系2和对照在叶色、叶毛、叶长和叶宽上均没有显著性差异。半致死温度分析结果表明,株系1、株系2以及对照叶片半致死温度(LT₅₀)分别为-6.646,-6.546和-5.351℃,处理与对照之间差异显著,且都低于对照,但株系1和株系2之间无显著性差异。SRAP的结果表明,假俭草低温诱导的株系1和株系2在110,230以及240 bp处均存在相同特征带,表明假俭草体细胞突变体植株的变异稳定且在分子水平与对照存在差异,但株系1和株系2无差异。因此,株系1和株系2可作为同一体细胞抗寒突变体株系加以利用。

Abstract: Weak cold-resistance of centipedegrass is one of the major factors limiting its wide application. This study aimed to obtain cold-resistant somatic mutants from the seed of eminent Chinese native centipedegrass selection E-126 by induction and screening at low temperature. The callus induced by seeds of centipedegrass was cultivated at 0℃ for 26 d during the process of subculture and two pieces of survival callus tissues were obtained. Strain 1 and strain 2 were obtained after survival callus subculture proliferation, differentiation, rhizogenesis and transplantation. No significant differences were found in leaf color, lamellar hair, leaf length and leaf width between strains 1and 2 and the control. LT₅₀ of strains 1 and 2 were -6.646 and -6.546 ℃, respectively, which was significantly lower than that of control plants (-5.351 ℃) and there was no significant difference between the LT₅₀ of strains 1 and 2. The results of sequence related amplified polymorphism (SRAP) markers demonstrated that there were identical characteristic bands at 110, 230 and 240 bp of centipedegrass strains 1 and 2 obtained through low temperature induction. However, no characteristic bands at 110, 230 and 240 bp were found in control plants, indicating that the somatic mutants of centipedegrass were genetically stable compared with that of control plants at a molecular level. Strains 1 and 2 could be utilized as identical strains.

中图分类号:

袁学军,王志勇,郑轶琦,刘建秀,佘建明. 假俭草体细胞抗寒突变体的获得及其SRAP分子鉴定[J]. 草业学报, 2011, 20(6): 237-244.

YUAN Xue-jun, WANG Zhi-yong, ZHENG Yi-qi, LIU Jian-xiu, SHE Jian-ming. Acquisition and identification of cold-resistant somatic mutants of centipedegrass[J]. Acta Prataculturae Sinica, 2011, 20(6): 237-244.

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