利用EST-SSR标记分析西南扁穗牛鞭草种质的遗传多样性

摘要/Abstract

摘要： 利用禾谷作物EST-SSR标记对采自我国西南地区的40份野生扁穗牛鞭草和3份扁穗牛鞭草国审品种的遗传变异和亲缘关系进行了研究。试验筛选出23对引物对43份供试材料进行扩增,共获得323条带,其中多态性条带261条,多态性条带比率(PPB)达80.4%,多态信息含量(PIC)为0.354~0.500,平均值为0.474,遗传相似系数(GS)为0.690~0.913,表现出丰富的遗传多样性。聚类分析结果表明,各供试材料间的聚类与其地理来源及形态特征具有一定的相关性。5 个扁穗牛鞭草地理类群间的分子方差分析(AMOVA)揭示了供试的扁穗牛鞭草类群内的遗传变异占总变异的95.32%,类群间变异占总变异的4.68%。表明禾谷作物的EST-SSR能用于扁穗牛鞭草遗传多样性研究,是一种有效的分子标记。本研究结果为扁穗牛鞭草种质的收集、利用及育种提供了理论依据。

Abstract: Using EST-SSR with primers from cereal crops, the genetic diversity was assessed in 40 wild Hemarthria compressa clones from southwestern China and three varieties. After amplification with 23 pairs of EST-SSR primers of Poaceae, 323 fragments were detected, of which 261 were polymorphic, accounting for 80.4% of the total. The polymorphic information content was from 0.354 to 0.500. The genetic similarity coefficient of 43 accessions ranged from 0.690 to 0.913. Clustering analysis also revealed that the clustering among tested materials was related to their geographic origins, ecological types and morphological characteristics. AMOVA (analysis of molecular variance) of five geographical groups of H. compressa revealed that 95.32% of total variance occurred within these groups and 4.68% of total variance occurred between them. It is suggested that using EST-SSR primers from cereal crops, is feasible and effective for analyzing the genetic diversity of H.compressa.

中图分类号:

S812-05
Q943

陈永霞,张新全,谢文刚,马啸,刘影. 利用EST-SSR标记分析西南扁穗牛鞭草种质的遗传多样性[J]. 草业学报, 2011, 20(6): 245-253.

CHEN Yong-xia, ZHANG Xin-quan, XIE Wen-gang, MA Xiao, LIU Ying. Genetic diversity of Hemarthria compressa germplasms from southwestern China based on EST-SSR markers[J]. Acta Prataculturae Sinica, 2011, 20(6): 245-253.

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