Abstract: The small-volume solution culture method was used to evaluate the aluminum (Al) tolerance of 51 centipedegrass accessions and two cultivars (‘TifBlair’ and ‘Common’) from the USA as a basis for selecting centipedegrass resources with strong Al tolerance thus providing an experimental basis for futher genetic improvement. Significant growth differences were found among 53 accessions treated with 1 500 μmol/L Al, and the CVs of relative root weight, relative shoot weight, and relative total weight were 20.55%, 15.62% and 15.52%, respectively. Using the membership function value method, 51 centipedegrass accessions were classified into five groups with different levels of Al tolerance. Twenty-one accessions belonged to groups 1-3 (Al-tolerant type), accounting for 39.6% of the total accessions, 13 accessions belonged to group 5 (Al-sensitive type), accounting for 24.5%, and 19 accessions belonged to group 4 (intermediate Al-tolerant type), accounting for 35.9%. ‘TifBlair’ and ‘Common’ were intermediate types, indicating a good potential for developing the genetic resource of Al tolerance in centipedegrass.