结缕草属草坪草种质资源的耐盐性评价

草业学报 ›› 2012, Vol. 21 ›› Issue (4): 43-51.

结缕草属草坪草种质资源的耐盐性评价

李珊^1,2,陈静波²,郭海林²,宗俊勤²,张芳²,禇晓晴²,蒋乔峰²,丁万文²,刘建秀^2*

1.南京农业大学园艺学院,江苏南京 210095;
2.江苏省中国科学院植物研究所,江苏南京 210014

收稿日期:2011-05-26 出版日期:2012-04-25 发布日期:2012-08-20
通讯作者: E-mail:turfunit@yahoo.com.cn
作者简介:李珊(1987-),女,山东潍坊人,在读硕士。E-mail: lishano526@126.com
基金资助:
江苏省科技厅基础设施项目(BM2009905)资助。

Salinity tolerance evaluation of Zoysia turfgrass germplasm

LI Shan^1,2, CHEN Jing-bo², GUO Hai-lin², ZONG Jun-qin², ZHANG Fang², CHU Xiao-qing², JIANG Qiao-feng², DING Wan-wen², LIU Jian-xiu²

1.College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China;
2.Institute of Botany, Jiangsu Province & Chinese Academy of Sciences, Nanjing 210014, China

Received:2011-05-26 Online:2012-04-25 Published:2012-08-20

摘要/Abstract

摘要： 以相对绿叶盖度和相对枝叶修剪干重为指标,通过水培法系统地对7种103份结缕草属种质资源用20 g/L NaCl进行耐盐性评价。结果表明,供试材料间的耐盐性差异很大。以相对绿叶盖度进行比较,比‘马尼拉’和‘兰引3号’强的材料各有28份和46份;以相对枝叶修剪干重为指标,比‘马尼拉’和‘兰引3号’强的材料各有11份和25份。供试材料种间耐盐性最强的为太平洋结缕草,大穗结缕草、沟叶结缕草和细叶结缕草的耐盐性也较强,中华结缕草、结缕草和朝鲜结缕草的耐盐性中等或较弱。以相对绿叶盖度和相对枝叶修剪干重为指标对103份结缕草属种质资源进行聚类分析,将103份结缕草属种质资源分成三大类,其中Ⅰ类为极耐盐类型,包括Z5185和Z093两份材料;Ⅱ类的相对绿叶盖度和相对枝叶修剪干重的平均值均较高(>70%),进一步分为耐盐、中等耐盐2类;Ⅲ类相对绿叶盖度和相对枝叶修剪干重的平均值较低,在欧式距离3处将Ⅲ类分为弱耐盐、盐敏感2类。

Abstract: Salinity tolerance in 103 Zoysia accessions from seven species was assessed in hydroponic culture containing 20 g/L NaCl. The relative green leaf coverage and relative dry shoot clipping weight showed that there were differences in salinity tolerance between accessions. Using relative green leaf coverage as an indicator, there were 28 and 46 accessions with more salinity tolerance than ‘manilagrass’ and Zoysia japonica ‘Lanyin No.3’, respectively. When compared by relative dry shoot clipping weight, there were 11 and 25 accessions with more salinity tolerance than ‘manilagrass’ and Z. japonica ‘Lanyin No.3’, respectively. Z. pacifica was the most salinity tolerant species, Z. macrostachya, Z. matrella, and Z. tenuifolia had strong salinity tolerance, while Z. sinica, Z. japonica and Z. koreana were intermediate or sensitive. Clustering analysis of relative green leaf coverage and relative dry shoot clipping weight, indicated that the 103 Zoysia accessions could be divided into three groups: group Ⅰ (including Z5185 and Z093) was extremely saline tolerant; group Ⅱ had comparatively higher relative green leaf coverages and relative dry shoot clipping weights (>70%). It could be further divided into salinity tolerance and intermediate salinity tolerance; group Ⅲ had comparatively low relative green leaf coverage and relative dry shoot clipping weight, and could be divided into weak and sensitive salinity tolerance at a Euclidean distance of 3.

中图分类号:

李珊,陈静波,郭海林,宗俊勤,张芳,禇晓晴,蒋乔峰,丁万文,刘建秀. 结缕草属草坪草种质资源的耐盐性评价[J]. 草业学报, 2012, 21(4): 43-51.

LI Shan, CHEN Jing-bo, GUO Hai-lin, ZONG Jun-qin, ZHANG Fang, CHU Xiao-qing, JIANG Qiao-feng, DING Wan-wen, LIU Jian-xiu. Salinity tolerance evaluation of Zoysia turfgrass germplasm[J]. Acta Prataculturae Sinica, 2012, 21(4): 43-51.

参考文献

[1] 吴欣明, 王运琦, 刘建宁, 等. 羊茅属植物耐盐性评价及其对盐胁迫的生理反应[J]. 草业学报, 2007, 16(6): 67-73.
[2] 张飞, 丁建丽, 塔西甫拉提·特依拜, 等. 干旱区典型绿洲土壤盐渍化特征分析: 以渭干河-库车河三角洲为例[J]. 草业学报, 2007, 16(4): 34-40.
[3] 刘兴元, 龙瑞军, 尚占环. 草地生态系统服务功能及其价值评估方法研究[J]. 草业学报, 2011, 20(1): 167-174.
[4] 牛东玲, 王启基. 盐碱地治理研究进展[J]. 土壤通报, 2002, 33(6): 449-455.
[5] 任继周, 梁天刚, 林慧龙, 等. 草地对全球气候变化的响应及其碳汇潜势研究[J]. 草业学报, 2011, 20(2): 1-22.
[6] 李娜, 李志军, 李培环, 等. 不同盐浓度对3种结缕草K⁺、Na⁺含量和膜质过氧化的影响[J]. 中国农学通报, 2010, 26(10): 152-155.
[7] Marcum K B, Murdoch C L. Salinity tolerance mechanisms of six C₄ turfgrasses[J]. Journal of the American Society for Horticultural Science, 1994, 119:779-784.
[8] 郭海林, 刘建秀. 结缕草属植物育种进展概述[J]. 草业学报, 2004, 13(3): 106-112.
[9] Qian Y L, Engelke M C, Foster M J V. Salinity effects on Zoysiagrass cultivars and experimental lines[J]. Crop Science, 2000, 40:488-492.
[10] Marcum K B. Salt gland ion secretion: A salinity tolerance mechanism among five Zoysiagrass species[J]. Crop Science, 1998, 38:806-810.
[11] 陈静波, 阎君, 姜燕琴, 等. 暖季型草坪草优良选系和品种抗盐性的初步评价[J]. 草业学报, 2009, 18(5): 107-114.
[12] Akiyoshi M, Yaneshita M, Nagasawa R, et al. Sea water tolerance of Zoysiagrass in relation to morphological and genetic classification[J]. Grassland Science, 1998, 44(1):7-13.
[13] 景艳霞, 袁庆华. NaCl 胁迫对苜蓿幼苗生长及不同器官中盐离子分布的影响[J]. 草业学报, 2011, 20(2): 134-139.
[14] 李亚, 耿蕾, 刘建秀. 中国结缕草属植物抗盐性评价[J]. 草地学报, 2004, 12(1): 8-11.
[15] Lee G J, Yoo Y K, Kim K S. Comparative salt tolerance study in Zoysiagrasses Ⅱ. Interspecific comparison among eight zoysiagrasses (Zoysia spp.)[J]. Journal of Korea Society of Horticulture Science, 1994, 35(2): 178-185.
[16] 陈静波, 张婷婷, 阎君, 等. 短期和长期盐胁迫对暖季型草坪草新选系生长的影响[J]. 草业科学,2008,25(7): 109-113.
[17] 陈静波, 阎君, 郭海林, 等. 暖季型草坪草大规模种质资源抗盐性评价指标的选择[J]. 草业科学, 2008, 25(4): 95-99.
[18] Weng J H, Chen Y C. Variation of salinity tolerance in Zoysia clones collected from different habitats in Taiwan[J]. Plant Production Science, 2001, 4(4): 313-316.
[19] 宣继萍, 周志芳, 刘建秀, 等. 结缕草属植物种间关系的SSR分析[J]. 西北植物学报, 2008, 28(2): 249-255.
[20] 周志芳, 宣继萍, 刘建秀, 等. 结缕草属植物种间关系的等位酶研究[J]. 草地学报, 2007, 15(6): 577-581.
[21] Koyama T. Grasses of Japan and its Neighboring Regions An Identification Manual[M]. Tokyo: Kodansha Press, 1987: 287-291.
[22] Wu Z, Peter H R, Hong D. Flora of China (Volume 22)[M]. St. Louis: Missouri Botanical Garden Press, 2007: 496-498.
[23] 蔡建一, 马清, 周向睿, 等. Na⁺在霸王适应渗透胁迫中的生理作用[J]. 草业学报, 2011, 20(1): 89-95.
[24] 李三相, 周向睿, 王锁民. Na⁺在植物中的有益作用[J]. 中国沙漠, 2008, 28(23): 485-490.
[25] Yaneshita M, Nagasawa R, Engelke M C, et al. Genetic variation and interspecific hybridization among natural populations of zoysiagrasses detected by RFLP analyses of chloroplast and nuclear DNA[J]. Genes & Genetic Systems, 1997, 72(4): 173-179.
[26] 郭海林, 刘建秀, 朱雪花, 等. 结缕草属植物杂交育种及其杂种鉴定——同工酶的变异分析[J]. 草业学报, 2006, 15(6): 101-108.