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草业学报 ›› 2012, Vol. 21 ›› Issue (4): 62-71.

• 研究论文 • 上一篇    下一篇

NaCl胁迫对玉米耐盐系与盐敏感系萌发和幼苗生长的影响

彭云玲,李伟丽,王坤泽,王汉宁*   

  1. 甘肃省干旱生境作物学重点实验室 甘肃农业大学农学院,甘肃 兰州 730070
  • 收稿日期:2012-02-17 出版日期:2012-04-25 发布日期:2012-08-20
  • 通讯作者: E-mail:wanghn@gsau.edu.cn
  • 作者简介:彭云玲(1978-),女,河南南阳人,讲师,博士。E-mail:pengyunlingpyl@163.com
  • 基金资助:
    973计划课题(2012CB722902),甘肃省科技支撑计划项目(1011NKCA076),甘肃省干旱生境作物学重点实验室开放课题(GSCS-2010-10)和甘肃农业大学SRTP项目(20110128)资助。

Effects of salt stress on seed germination and seedlings growth of salt-tolerant line and salt-sensitive line of maize

PENG Yun-ling, LI Wei-li,WANG Kun-ze, WANG Han-ning   

  1. Gansu Provincial Key Laboratory of Aridland Crop Science; College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China
  • Received:2012-02-17 Online:2012-04-25 Published:2012-08-20

摘要: 不同NaCl浓度梯度,对玉米耐盐系8723、81162和盐敏感系P138的种子萌发和幼苗生长2个阶段进行胁迫处理。结果表明,在种子萌发阶段,随NaCl浓度的增加,与盐敏感系相比,玉米耐盐系的发芽率、发芽势、发芽指数、活力指数、胚芽长度较高,盐害指数较低,且变化幅度小。对幼苗生长阶段指标的测定表明,随着NaCl浓度的增加,耐盐系和盐敏感系株高、根长、鲜质量逐渐降低,但是耐盐系各个指标的测量值较盐敏感系高,且变化幅度小。对玉米耐盐系和盐敏感系在不同浓度盐胁迫下生理变化的研究结果表明,与盐敏感系相比, 玉米耐盐系相对含水量高、组织外渗液的相对电导率低, 且变化幅度小。综合所有指标的测定结果,耐盐系8723比81162有较强的耐盐性。

Abstract: Salt stress affects plant growth and development and reduces productivity of crop. Maize (Zea mays) is moderate sensitive to salt. Screening and studying maize germplasm resistant to salt have certain directive function on improving salt-tolerant germplasm resources and breeding salt-tolerant variety, and have certain theoretical meaning of genetic mechanism of salt tolerance in maize. The study will focus on the comprehensive comparison of salt-tolerant properties change of salt-tolerant lines 8723, 81162 and salt-sensitive line P138 at germination and seedling stages under salt stress, in order to probe into salt-tolerant mechanism in maize, to provide the materials for breeding resistant-salt variety and discovering genes related to salt tolerance and to lay the foundation for salt-tolerant genetic mechanism in maize.Seed germination and seedling growth of salt-tolerant lines 8723, 81162 and salt-sensitive line P138 of maize under different concentrations of NaCl stress were determined in this experiment. The experimental results indicated that, with the increase of salt concentration, there were higher germination rate, germination potential, germination index, activity index, plumule length, and lower salt injury index, of salt-tolerant line with a small change at seed germination stage, compared with salt-sensitive line. A study of salt-tolerant lines and salt-sensitive line under different concentrations of NaCl stress in seedling stage showed that the height of seedlings, root length, fresh weight of the three inbred lines all decreased with increasing concentrations of NaCl. The decrease rate of these indices for the salt-tolerant lines was all less than those for the salt-sensitive inbred line. The results of physiological changes under different concentrations of salt stress indicated that there were higher relative water content and lower relative conductivity of organization of external seepage of salt-tolerant lines with a small change, compared with salt-sensitive line. To analyze all experimental results, salt-tolerant line 8723 had stronger tolerant than 81162.

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