毛细辛提取物体外抗氧化活性研究

摘要/Abstract

摘要： 通过毛细辛不同溶剂提取物体外抗氧化活性研究,为进一步开发毛细辛的药用价值提供参考。用50%的乙醇回流提取毛细辛的粉末,减压浓缩过滤得到乙醇浸膏,加蒸馏水制成悬浮液,依次用等体积的石油醚,乙酸乙酯,正丁醇萃取,得到石油醚,乙酸乙酯,正丁醇和水提物。以2,6-二叔丁基对甲酚(BHT)为对照,分别采用DPPH法、Fenton法、NBT还原法和Fe²⁺螯合法测定4种提取物的抗氧化活性。结果表明,毛细辛4种溶剂提取物均有不同程度的抗氧化活性,且活性与浓度成正相关。毛细辛乙酸乙酯提取物的1,2-二苯基-2-苦苯肼自由基(DPPH·)的清除活性(IC₅₀值0.376 mg/mL),超氧阴离子自由基(O₂^-·)的清除活性(IC₅₀值0.297 mg/mL)均最强。正丁醇提取物的羟基自由基(·OH)清除活性(IC₅₀值0.776 mg/mL)为最强,水提物的金属离子螯合活性(IC₅₀值0.026 mg/mL)为最强,远超过对照BHT(0.373 mg/mL)。实验表明,毛细辛乙酸乙酯提取物,正丁醇提取物和水提物均有较强的自由基清除活性,可作为新的自由基清除剂;水提物可以作为新的金属离子螯合剂。

Abstract: The antioxidative activities in vitro of different solvent extracts of Asarum himalaicum were studied to provide more information for future research on the medicinal value of A. himalaicum. The powdered material of A. himalaicum was extracted with 50% ethanol refluxing extraction and the ethanol condensate was evaporated to dryness under vacuum on a rotary evaporator. The condensate was then extracted with Petroleum ether, ethyl acetate or n-butanol or water at the same volume to get four different extracts. The four extracts were then examined for their antioxidative activities by DPPH (1,1-diphenyl-2-plcrylhydrazyl), Fenton, NBT (nitrotetrazolium blue chloride) reduction and Fe²⁺ chelation. The results suggest that the four solvent extracts of A. himalaicum had a certain degree of antioxidant activity, and the activity had a positive correlation with the concentration of the extract. The ethyl acetate extract had higher activity in scavenging DPPH·(IC₅₀ =0.376 mg/mL) and O₂^-· (IC₅₀=0.297 mg/mL Hile the n-butanol extract had the highest ·OH scavenging capacity (IC₅₀=0.776 mg/mL). The water extract was the most powerful for chelating metal ions (IC₅₀=0.026 mg/mL) and was more powerfull than that of BHT (2, 6-di-tert-butyl-4-methylp-henol) (IC₅₀=0.373 mg/mL). This study has shown that the ethyl acetate extract, n-butanol extract and water extract of A. himalaicum have remarkable free radical scavenging activities, and the water extract can be selected as a new metal ion chelating reagent.

中图分类号:

Q943.1

何全磊,苗芳,李春玲. 毛细辛提取物体外抗氧化活性研究[J]. 草业学报, 2012, 21(5): 285-290.

HE Quan-lei, MIAO Fang, LI Chun-ling. Antioxidant activities in vitro of different polar solvent extracts of Asarum himalaicum[J]. Acta Prataculturae Sinica, 2012, 21(5): 285-290.

参考文献

[1] Cerutti P A. Oxidant stress and carcinogenesis[J]. European Journal of Clinical Investigation, 1991, 21: 1-11.
[2] Halliwell B, Gutteridge J M C. Free Radicals in Biology and Medicine(3rd ed)[M]. London: Oxford University Press, 1999.
[3] Farber J L. Mechanisms of cell injury by activated oxygen species[J]. Environmental Health Perspectives, 1994, 102: 17-24.
[4] Cerutti P A. Prooxidant states and tumor promotion[J]. Science, 1985, 227: 379-381.
[5] Fridovich I. Fundamental aspects of reactive oxygen species, or what’s the matter with oxygen[J]. Annals of the New York Academy of Sciences, 1999, 893: 13-18.
[6] 邵旭平, 万建宏, 万东石. 不同生态型胡杨异形叶对虫害的抗氧化反应[J].草业科学, 2011, 28(7): 1396-1399.
[7] 赵亚民, 张丽静, 傅华. 维生素E在饲草及畜产品中的应用研究[J].草业科学, 2011, 28(6): 1167-1172.
[8] Aruoma O I. Nutrition and health aspect of free radicals and antioxidants[J]. Food and Chemical Toxicology, 1994, 32: 671-683.
[9] Pezzuto J M, Park E J. Autoxidation and antioxidants[A]. In: Swarbrick J, Boylan J C. Encyclopedia of Pharmaceutical Technology[C]. New York: Marcel Dekker, 2002: 97-113.
[10] Ames S N, Shigrenaga M K, Hagen T M. Oxidant, antioxidant and degenerative disease of aging[J]. Proceedings of the National Academy of Sciences of the United States of America, 1993, 90: 7915-7922.
[11] Delectis Florae Reipublicae Popularis Sinicae Agendae Academiae Sinicae Edita. Flora Republicae Popularis Sinicae (中国植物志), Tomus 24[M]. Beijing: Science Press, 1988: 165.
[12] 罗文蓉, 牛永红. 甘肃细辛的生药鉴定[J].甘肃中医, 1999, 12(1): 36-37.
[13] 韩俊艳, 孙川力, 纪明山. 中药细辛的研究进展[J].中国农学通报, 2011, 27(9): 46-50.
[14] 谢百波, 尚明英, 王璇, 等. 单叶细辛中一个新的马兜铃酸类化合物[J].药学学报, 2011, 46 (2): 188-192.
[15] 袁晓琴,孙莲芬.细辛不同提取部位镇痛作用及毒性的比较研究[J].时珍国医国药, 2009, 20(8): 2050-2051.
[16] Sarikurkcu C, Tepe B, Semiz D K, et al. Evaluation of metal concentration and antioxidant activity of three edible mush rooms from Mugla, Turkey[J]. Food and Chemical Toxicology, 2010, 48 (5): 1230-1233.
[17] Wei Q, Ma X H, Zhao Z, et al. Antioxidant activities and chemical profiles of pyroligneous acids from walnut shell[J]. Journal of Analytical and Applied Pyrolysis, 2010, 88(2): 149-154.
[18] Ozsoy N, Can N, Yanardag R, et al. Antioxidant activity of Smilax excelsa L. leaf extracts[J]. Food Chemistry, 2008, 110(3): 571-583.
[19] Gülin I, Berashvili D, Gepdiremen A. Antiradical and antioxidant activity of total anthocyanins from Perilla pankinensis decne[J]. Journal of Ethnopharmacology, 2005, 101: 287-293.
[20] Moyer R A, Hummer K E, Finn C E, et al. Anthocyanins, phenolics and antioxidant capacity in diverse small fruits: Vaccinium, Rubus and Ribes[J]. Journal of Agricultural and Food Chemistry, 2002, 50: 166-171.
[21] 谢云涛, 马国平, 何玉凤, 等. BPR 硫脲催化光度法对羟基自由基的检测及清除作用[J]. 化学通报, 2006, 6(6): 458-461.
[22] 刘璐, 付明哲, 王侠, 等. 5种棘豆总黄酮含量的测定比较[J]. 草业科学, 2011, 28(4): 683-686.
[23] 冯涛, 庄海宁. 黄酮类化合物结构特征与抗氧化性关系研究进展[J]. 粮食与油脂, 2008, (10): 8-11.
[24] 谭榀新, 叶涛, 刘湘新, 等. 植物提取物抗氧化成分及机理研究进展[J]. 食品科学, 2010, 31(15): 288-292.
[25] Dan Y, Liu H Y, Gao W W, et al. Activities of essential oils from Asarum heterotropoides var. mandshuricum against five phytopathogens[J]. Crop Protection, 2010, 29(3): 295-299.
[26] 沈映君, 张世芳, 张世玮.中药药理学[M]. 上海: 上海科学技术出版社, 1997: 32-33.
[27] 扈成浩, 薛敦渊, 李兆林, 等.宁夏毛细辛挥发油化学成分研究[J]. 兰州大学学报(自然科学版), 1989, 25(2): 59.
[28] Cai S Q, Yu J, Wang X, et al. Cytotoxic activity of some Asarum plants[J]. Fitoterapia, 2008, 79: 293-297.
[29] Kim S J, Zhang C G, Lim J T. Mechanism of anti-nociceptive effects of Asarum sieboldii Miq. Radix: potential role of bradykinin, histamine and opioid receptor-mediated pathways[J]. Journal of Ethnopharmacology, 2003, 88: 5-9.
[30] Iwashinaa T, Kitajima J, Shiuchi T, et al. Chalcones and other flavonoids from Asarum sensulato (Aristolochiaceae)[J]. Biochemical Systematics and Ecology, 2005, 33: 571-584.
[31] Lin B Q, Chiou G C. Antioxidant activity of naringenin on various oxidants induced damages in ARPE219 cells and HUVEC[J]. International Journal of Ophthalmology (IJO), 2008, 8(10): 1963-1967.
[32] Schaneberg B T, Khan I A. Analysis of products suspected of containing Aristolochia or Asarum species[J]. Journal of Ethnopharmacology, 2004, 94: 245-249.