耐药性木霉T2菌株的筛选、紫外诱变与药剂驯化

草业学报 ›› 2013, Vol. 22 ›› Issue (2): 117-122.

耐药性木霉T2菌株的筛选、紫外诱变与药剂驯化

尹婷,徐秉良^*,梁巧兰,古丽君,李荣峰

甘肃农业大学草业学院草业生态系统教育部重点实验室甘肃省草业工程实验室中-美草地畜牧业可持续发展研究中心, 甘肃兰州 730070

收稿日期:2011-10-21 出版日期:2013-02-25 发布日期:2013-04-20
通讯作者: E-mail:xubl@gsau.edu.cn
作者简介:尹婷(1985-),女,甘肃山丹人,在读硕士。E-mail:yintingting@st.gsau.edu.cn
基金资助:
草业生态系统教育部省部共建重点实验室项目(CY-GG-2006-013),甘肃省农牧厅生物技术专项(GNSW-2009-04)和甘肃省教育厅项目(042-03)资助。

UV mutagenesis and screening for fungicide resistant strains of Trichoderma aureoviride T2

YIN Ting, XU Bing-liang, LIANG Qiao-lan, GU Li-jun, LI Rong-feng

College of Grassland Science, Gansu Agricultural University, Key Laboratory of Grassland Ecosystem, Ministry of Education, Pratacultural Engineering Laboratory of Gansu Province, Sino-U.S. Centers for Grazingland Ecosystem Sustainability, Lanzhou 730070, China

Received:2011-10-21 Online:2013-02-25 Published:2013-04-20

摘要/Abstract

摘要： 为获得对杀菌剂有抗药性的菌株,首先测定了深绿木霉T2菌株对6种常用杀菌剂的抗性,选择出了最敏感的杀菌剂－速克灵。同时通过紫外光照射挑选出突变菌株,并在不同浓度的速可灵(50～800 μg/mL)培养基上驯化,最后得到了4株对速克灵抗药性较强的菌株:T2-1,T2-2,T2-5和T2-6。10次转化、菌落生长速度测定、产孢数量的测定和对灰葡萄孢菌的拮抗作用测定结果表明,此4个菌株生物学特性稳定,其中T2-6菌株显著优于其他3株。

Abstract: To obtain fungicide resistant strains, Trichoderma aureoviride strain T2 was tested against six fungicides and the most effective fungicide (Procymidone) was selected for screening against mutagenic strains derived by UV irradiation. The strains were grown on a range of procymidone concentrations (50-800 μg/mL) on a medicinal culture medium. Four strongly resistant strains (T2-1, T2-2, T2-5, and T2-6) were obtained. After the 10 inoculation, colony growth rate, sporulation and antagonism to Botrytis cinerea were assessed and the biological characteristics of the four strains were found to be constant. The T2-6 strain was significantly better than the other three strains.

中图分类号:

Q948.1

尹婷,徐秉良,梁巧兰,古丽君,李荣峰. 耐药性木霉T2菌株的筛选、紫外诱变与药剂驯化[J]. 草业学报, 2013, 22(2): 117-122.

YIN Ting, XU Bing-liang, LIANG Qiao-lan, GU Li-jun, LI Rong-feng. UV mutagenesis and screening for fungicide resistant strains of Trichoderma aureoviride T2[J]. Acta Prataculturae Sinica, 2013, 22(2): 117-122.

参考文献

[1] 张茹, 李金华, 柴兆祥, 等. 甘肃河西马铃薯根际生防木霉菌对接骨木镰刀菌的拮抗筛选及鉴定[J]. 草业学报, 2009, 18(2): 138-145.
[2] 王勇, 杨秀荣, 刘水芳. 拮抗木霉耐药性菌株的筛选及其与速克灵防治灰霉病的协同作用[J]. 天津农学院学报, 2002, 9(4): 19-22.
[3] 古丽君, 徐秉良, 梁巧兰, 等. 生防木霉菌T2菌株对禾草腐霉病抑菌作用及机制研究[J]. 草业学报, 2011, 20(2): 46-51.
[4] 高书晶, 刘爱萍, 徐林波, 等. 8种生物农药对草原蝗虫的田间防治效果评价[J]. 草业科学, 2011, 28(2): 304-307.
[5] 胡冠芳, 刘敏艳, 李玉奇, 等. 甘肃天然草地30种有毒植物提取物对菜粉碟幼虫的拒食和触杀作用研究[J]. 草业学报, 2011, 20(5): 169-176.
[6] 孙娈姿, 王佺珍, 呼天明, 等. 菊苣叶提取物对粘虫的生物活性研究[J]. 草业学报, 2011, 20(2): 60-66.
[7] Baker R. Diversity in biological control[J]. Crop Protection, 1991, 10: 85-94.
[8] Lewis J A, Papavizas G C. Biocontrol of plant disease: the approach for tomorrow[J]. Crop Protection, 1991, 10: 1086-1105.
[9] Harman G E. The genetic nature and biocontrol ability of progeny from protoplast fusion in Trichoderma[A]. In: Chet I. Biotechnology in Plant Disease Control[C]. Wiley Liss Press, 1993: 237-255.
[10] 杨合同, 唐文华, 李纪顺, 等. 绿色木霉LTR-2菌株的紫外线改良[J]. 中国生物防治, 2004, 20(3): 182-186.
[11] 丁中, 刘峰, 慕立义. 紫外光诱导哈茨木霉产生腐霉利抗性菌株的研究[J]. 中国生物防治, 2002, 18(2): 75-78.
[12] 鲁海菊, 刘云龙, 张云霞. 哈茨木霉多菌灵耐药性菌株的筛选[J]. 云南农业大学学报, 2005, 20(3): 436-437.
[13] 田连生, 李贵香, 高玉爽. 紫外光诱导木霉产生对速克灵抗药性菌株的研究[J]. 中国植保导刊, 2006, 26(6): 18-20.
[14] 程东美, 张志祥, 区丽文, 等. 哈茨木霉T2菌株耐药性的测定及其对几种病原菌的抑制作用研究[J]. 安徽农业科学, 2008, 36(10): 4170-4172.
[15] 田连生. 紫外光诱导哈茨木霉产生对多菌灵抗药性的菌株[J]. 农业环境科学学报, 2007, 26(1): 318-321.
[16] 田连生, 冯树波. 耐药性木霉菌株的筛选及其对灰霉病的防治[J]. 生物技术, 2005, 15(5): 26-28.
[17] 田连生. 抗药性木霉菌株的选育及其与多菌灵的协同作用[J]. 核农学报, 2008, 22(1): 32-35.
[18] 梁志怀, 魏林, 安哲宇, 等. 生防木霉菌耐三唑类杀菌剂菌株的选育[J]. 中国生物防治, 2010, 26(1): 60-65.
[19] 覃柳燕, 蒋妮, 唐美琼, 等. 木霉菌对山豆根根腐病菌的拮抗作用[J]. 中药材, 2011, 34(4): 499-502.
[20] 孙冬梅, 杨谦, 张军政. 黄绿木霉诱变菌株对大豆根腐病镰刀菌的拮抗[J]. 大豆科学, 2005, 24(3): 171-175.
[21] Papvizas G C. Evaluation of new biotypes of Trichderma harziaum for tolerance tobenomyl enhanced capabilities[J]. Phytopathology, 1982, 72: 126-132.
[22] Papvizas G C. Physiological and biocontrol characteristis of stable mutants of Trichoderma viride resistant to MBC fungicides[J]. Phytopathology, 1983, 73: 407-411.
[23] 陈伯清, 屈海泳, 刘连妹. 木霉菌在园艺植物上的应用研究进展[J]. 安徽农业科学, 2008, 36(12): 4960-4963.
[24] 于雪云, 扈进冬, 杨合同. 木霉菌抗药性研究进展[J]. 山东农业科学, 2007, 6: 81-85.