Abstract: The aim of the experiment was to explore the regulatory role of 6-benzylaminopurine (6-BA))on resistance ability of low temperature and weak light in pepper seedlings (Capsicum annuum).The effects of 0.08 mmol/L 6-BA pretreatment on gas exchange parameters, chlorophyll content, endogenous hormone content in pepper seedlings leaves under low temperature (15℃/5℃) and weak light (100 μmol/m²·s) stress were investigated. Results showed that the photosynthetic rate (Pn), stomatal conductance (Gs), chlorophyll a (Chl a) content, chlorophyll b (Chl b) content, total chlorophyll (Chl T) content and zeatin riboside(ZR) content of pepper seedling leaves were significantly declined, but intercellular CO₂ concentration (Ci), abscisic acid(ABA) content were significantly increased under low temperature and weak light stress. The reduction in photosynthesis rate caused by low temperature and weak light stress was considered to be a result of non-stomatal restriction mainly. 6-BA had no changed trend of the above index with treatment time prolonged. Under low temperature and weak light stress, treatment with 6-BA significantly declined Ci, ABA content compared with control, but Pn, Gs, Chl a content, Chl b content, Chl T content and ZR content in previous 6 days of strss period were significantly increased.The results suggested that pretreatment with 0.08 mmol/L 6-BA had obviously role on enhanced pepper seedlings tolerance to low temperature and weak light, especially for pepper cultivar were sensitive to low temperature and weak light stress.