西藏不同饲草全混合日粮发酵品质和有氧稳定性的研究

doi:10.11686/cyxb20140612

摘要/Abstract

摘要： 为了充分利用西藏的农作物秸秆和饲草资源,本试验在当地奶牛场采用的以全株玉米和青稞秸秆为主要粗饲料的TMR配方基础上,用苜蓿、全株燕麦和小麦秸秆按不同比例替代全株玉米。青贮发酵45 d后评定各组发酵品质、营养特性和有氧稳定性。结果表明,发酵45 d后,TMR-2、TMR-3和TMR-4组pH值显著高于TMR-1(P<0.05),乳酸含量均低于TMR-1,但各替代组仅检测到微量丙酸和丁酸含量,且氨态氮/总氮较低,表明各组发酵品质均良好。发酵后各组水溶性碳水化合物含量均有大幅度下降,除TMR-1组外,其他3组干物质含量基本不变,粗蛋白、中性洗涤纤维、酸性洗涤纤维和粗脂肪等营养物质含量不同程度增加,其中TMR-2和 TMR-3组粗蛋白含量高于15%,能够满足改良奶牛的营养需要。在有氧暴露过程中,各组乳酸、乙酸和水溶性碳水化合物含量均出现不同程度的下降,丙酸和丁酸含量在有氧暴露第3天均有升高,之后逐渐下降。在有氧暴露9 d中,pH值均呈上升趋势,其中TMR-3组pH值上升幅度最小,其有氧稳定性最好。综上所述,虽然TMR-3发酵品质略低于TMR-1组,但其有氧稳定性有所提高,因此,TMR-3组可应用于生产实际中。

Abstract: The objective of this study was to identify opportunities to improve the utilization of crop straw and forage resources in Tibet. The experiment was based on the total mixed ration (TMR) typically used in local dairy systems, comprising whole-crop corn and barley straw (TMR-1), comparing substitutes for whole-crop corn; alfalfa (TMR-2), whole-crop oat (TMR-3) or wheat straw (TMR-4) at different ratios. After 45 d fermentation, pH values of TMR-2, TMR-3 and TMR-4 treatments were significantly (P<0.05) higher than that of TMR-1, while corresponding lactic acid contents were lower. Propionic acid and butyric acid were very low in all treatments except TMR-1 and ammonia nitrogen/total nitrogen was <100 g/kg TN, indicating that fermentation was good in all treatments. Water-soluble carbohydrate content in all treatments was significantly lower (P<0.05) after 45 d of fermentation. The dry matter content of all treatments except TMR-1 remained constant,while crude protein, neutral detergent fiber, acid detergent fiber and ether extract increased in all treatments. The crude protein content of TMR-2 and TMR-3 were above 15%, sufficient for dairy production. During aerobic exposure, lactic acid, acetic acid and water-soluble carbohydrate content of all treatments declined. Propionate and butyrate content initially increased after three days of aerobic exposure and then gradually decreased in all treatments. After 9 d of aerobic exposure, the pH increased but the increase was minimal in TMR-3, indicating good aerobic stability. It was concluded that TMR-3 group could be used to produce TMR in Tibet.

中图分类号:

S816.6

王勇,原现军,郭刚,闻爱友,王坚,肖慎华,余成群,巴桑,邵涛. 西藏不同饲草全混合日粮发酵品质和有氧稳定性的研究[J]. 草业学报, 2014, 23(6): 95-102.

WANG Yong,YUAN Xian-jun,GUO Gang,WEN Ai-you,WANG Jian,XIAO Shen-hua,YU Cheng-qun,BA Sang,SHAO Tao. Fermentation and aerobic stability of mixed ration forages in Tibet[J]. Acta Prataculturae Sinica, 2014, 23(6): 95-102.

参考文献

Reference：
[1]Yuan X J, Shao T, Yu C Q, et al.A study on fermentation quality of mixed silages of hulless barley straw and perennial ryegrass in Tibet[J]. Acta Prataculturae Sinica, 2012, 21(4): 325-330.
[2]Yuan X J, Wang Q, Shao T, et al. Effect of molasses addition on fermentation and nutritive quality of mixed silage of hulless barley straw and perennial ryegrass in Tibet[J]. Acta Prataculturae Sinica, 2013, 22(3):116-123.
[3]Kolver E S, Muller L D. Performance and nutrient intake of high producing holstein cows consuming pasture or a total mixed ration[J]. Journal of Dairy Science, 1998, 81(5):1404-1411.
[4]Nishino N, Wang F J. Resistance to aerobic deterioration of total mixed ration silage:effect of ration formulation, air infiltration and storage period on fermentation characteristics and aerobic stability[J]. Journal of the Science of Food and Agriculture, 2008, 88(1): 133-140.
[5]Wang F, Nishino N. Ensiling of soybean curd residue and wet brewers grains with or without other feeds as a total mixed ration[J]. Journal of Dairy Science, 2008, 91(6):2380-2387.
[6]Weinberg Z G, Chen Y, Miron D, et al. Preservation of total mixed rations for dairy cows in bales wrapped with polyethylene stretch film-A commercial scale experiment[J]. Animal Feed Science and Technology, 2011, 164(1):125-129.
[7]Sepp l A, Heikkil T, Mki M, et al. Controlling aerobic stability of grass silage-based total mixed rations[J]. Animal Feed Science and Technology, 2013, 179(3):54-60.
[8]Rong H, Shao T, Yu C Q, et al. Effects of FJLB, LAB and glucose addition on fermentation quality of napier grass (Pennisetum purpureum) silage[J]. Acta Prataculturae Sinica, 2013, 22(3):108-115.
[9]McDonald P, Playne M J. The buffering constituents of herbage and of silage[J]. Journal of the Science of Food and Agriculture, 1966, 17(6):264-265.
[10]Barker S B, Summerson W H. The colorimetric determination of lactic acid in biological material[J]. Journal of Biological Chemistry, 1941, 138:535-554.
[11]Broderica G A, Kang J H. Automated simultaneous determination of ammonia and amino acids in ruminal fluid and in vitro media[J]. Journal of Dairy Science, 1980, 33(1):64-75.
[12]Porter M G, Murray R S. The volatility of components of grass silage on oven drying and the inter-relationship between dry-matter content estimated by different analytical methods[J]. Grass and Forage Science, 2001, 56(4):405-411.
[13]Meeshe R, Merwe G D, Grayling J F, et al. The effect of adding an enzyme containing lactic acid bacterial inoculant to big round bale oat silage on silage, milk production and milk composition of jersey cows[J]. Animal Feed Science and Technology, 2002, 97(3):159-167.
[14]Shao T, Shimojo M, Masuda Y, et al. Effect of adding glucose, sorbic acid and pre-fermented juices on the fermentation quality of guineagrass[J]. Asian-Australasian Journal of Animal Sciences, 2005, 18(9):1273-1278.
[15]Shao T, Noriko O H B A, Masuda Y, et al. Fermentation quality of forage oat silages treated with pre-fermented juices, sorbic acid, glucose and encapsulated-glucose[J]. Journal of Faculty of Agriculture, 2003, 47(2):341-349.
[16]Yu J, Zheng C, Li F D, et al.An evaluation of mixed-ensiling sunflower straw and whole corn on silage quality[J]. Acta Prataculturae Sinica, 2013, 22(5):198-204.
[17]McDonald P. The Biochemistry of Silage[M]. University of Wisconsin:Chalcombe Publications, 1991: 1-10.
[18]Johnson L M, Harrison J H, Davidson D, et al. Corn silage management:effect of maturity, inoculation, and mechanical processing on pack density and aerobic stability[J]. Journal of Dairy Science, 2002, 85(2):434-444.
[19]Danner H, Holzer M, Mayrhuber E, et al. Acetic acid increases stability of silage under aerobic conditions[J]. Applied and Environmental Microbiology, 2003, 69(1):562-567.
[20]Kung L, Sheperd A C, Smagala A M, et al. The Effect of preservatives based on propionic acid on the fermentation and aerobic stability of corn silage and a total mixed ration[J]. Journal of Dairy Science, 1998, 81(5):1322-1330.
[21]Nishino N, Wada H, Yoshida M, et al. Microbial counts, fermentation products, and aerobic stability of whole crop corn and a total mixed ration ensiled with and without inoculation of Lactobacillus casei or Lactobacillus buchneri[J]. Journal of Dairy Science, 2004, 87(8):2563-2570.

参考文献：
[1]原现军, 邵涛, 余成群, 等. 西藏青稞秸秆与多年生黑麦草混合青贮发酵品质的研究[J]. 草业学报, 2012, 21(4): 325-330.
[2]原现军, 王奇, 邵涛, 等. 添加糖蜜对青稞秸秆和多年生黑麦草混合青贮发酵品质及营养价值的影响[J]. 草业学报, 2013, 22(3):116-123.
[3]Kolver E S, Muller L D. Performance and nutrient intake of high producing holstein cows consuming pasture or a total mixed ration[J]. Journal of Dairy Science, 1998, 81(5):1404-1411.
[4]Nishino N, Wang F J. Resistance to aerobic deterioration of total mixed ration silage:effect of ration formulation, air infiltration and storage period on fermentation characteristics and aerobic stability[J]. Journal of the Science of Food and Agriculture, 2008, 88(1): 133-140.
[5]Wang F, Nishino N. Ensiling of soybean curd residue and wet brewers grains with or without other feeds as a total mixed ration[J]. Journal of Dairy Science, 2008, 91(6):2380-2387.
[6]Weinberg Z G, Chen Y, Miron D,et al. Preservation of total mixed rations for dairy cows in bales wrapped with polyethylene stretch film-A commercial scale experiment[J]. Animal Feed Science and Technology, 2011, 164(1):125-129.
[7]Sepp l A, Heikkil T, M ki M,et al. Controlling aerobic stability of grass silage-based total mixed rations[J]. Animal Feed Science and Technology, 2013, 179(3):54-60.
[8]荣辉, 邵涛, 余成群, 等. 添加绿汁发酵液、乳酸菌制剂和葡萄糖对象草青贮发酵品质的影响[J]. 草业学报, 2013, 22(3):108-115.
[9]McDonald P, Playne M J. The buffering constituents of herbage and of silage[J]. Journal of the Science of Food and Agriculture, 1966, 17(6):264-265.
[10]Barker S B, Summerson W H. The colorimetric determination of lactic acid in biological material[J]. Journal of Biological Chemistry, 1941, 138:535-554.
[11]Broderica G A, Kang J H. Automated simultaneous determination of ammonia and amino acids in ruminal fluid and in vitro media[J]. Journal of Dairy Science, 1980, 33(1):64-75.
[12]Porter M G, Murray R S. The volatility of components of grass silage on oven drying and the inter-relationship between dry-matter content estimated by different analytical methods[J]. Grass and Forage Science, 2001, 56(4):405-411.
[13]Meeshe R, Merwe G D, Grayling J F,et al. The effect of adding an enzyme containing lactic acid bacterial inoculant to big round bale oat silage on silage, milk production and milk composition of jersey cows[J]. Animal Feed Science and Technology, 2002, 97(3):159-167.
[14]Shao T, Shimojo M, Masuda Y,et al. Effect of adding glucose, sorbic acid and pre-fermented juices on the fermentation quality of guineagrass[J]. Asian-Australasian Journal of Animal Sciences, 2005, 18(9):1273-1278.
[15]Shao T, Noriko O H B A, Masuda Y,et al. Fermentation quality of forage oat silages treated with pre-fermented juices, sorbic acid, glucose and encapsulated-glucose[J]. Journal of Faculty of Agriculture, 2003, 47(2):341-349.
[16]于杰, 郑琛, 李发弟, 等. 向日葵秸秆与全株玉米混合青贮饲料品质评定[J]. 草业学报, 2013, 22(5):198-204.
[17]McDonald P. The Biochemistry of Silage[M]. University of Wisconsin:Chalcombe Publications, 1991: 1-10.
[18]Johnson L M, Harrison J H, Davidson D,et al. Corn silage management:effect of maturity, inoculation, and mechanical processing on pack density and aerobic stability[J]. Journal of Dairy Science, 2002, 85(2):434-444.
[19]Danner H, Holzer M, Mayrhuber E,et al. Acetic acid increases stability of silage under aerobic conditions[J]. Applied and Environmental Microbiology, 2003, 69(1):562-567.
[20]Kung L, Sheperd A C, Smagala A M,et al. The Effect of preservatives based on propionic acid on the fermentation and aerobic stability of corn silage and a total mixed ration[J]. Journal of Dairy Science, 1998, 81(5):1322-1330.
[21]Nishino N, Wada H, Yoshida M,et al. Microbial counts, fermentation products, and aerobic stability of whole crop corn and a total mixed ration ensiled with and without inoculation of Lactobacillus caseior Lactobacillus buchneri[J]. Journal of Dairy Science, 2004, 87(8):2563-2570.

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