多年生黑麦草愈伤组织航天搭载后再生株系的抗旱性研究

doi:10.11686/cyxb20150109

草业学报 ›› 2015, Vol. 24 ›› Issue (1): 64-74.DOI: 10.11686/cyxb20150109

多年生黑麦草愈伤组织航天搭载后再生株系的抗旱性研究

彭丽梅^{1, 2}, 曹丽^{1, 3}, 韩蕾¹, 钱永强¹, 孙振元^{1*, *}

1.中国林业科学研究院林业研究所,国家林业局林木培育重点实验室,北京100091;
2.棕榈园林股份有限公司,上海 200433;
3.延边大学农学院,吉林延吉 133002

收稿日期:2013-11-11 出版日期:2015-01-20 发布日期:2015-01-20
通讯作者: sunzy@caf.ac.cn
作者简介:彭丽梅(1987-),女,湖南隆回人,研究实习员,硕士。E-mail:penglimeihuagong@163.com
基金资助:
国家“863”计划项目(2007AA100105,2009AA102109)资助

Drought resistance analysis of regenerated plants from calli of Lolium perenne carried by the Shenzhou No.7 spaceship

PENG Limei^{1, 2}, CAO Li^{1, 3}, HAN Lei¹, QIAN Yongqiang¹, SUN Zhenyuan^{1, *}

1.Research Institute of Forestry, Chinese Academy of Forestry, Key Laboratory of Forest Cultivation, State Forestry Administration, Beijing 100091, China;
2.Palm Landscape Architecture Co., Ltd., Shanghai 200433, China;
3.College of Agriculture Yanbian University, Yanji 133002, China

Received:2013-11-11 Online:2015-01-20 Published:2015-01-20

摘要/Abstract

摘要： 利用“神舟七号”飞船搭载多年生黑麦草品种(Derby)的胚性愈伤组织经空间诱变后,从搭载再生株系(SP)群体中获得了3个抗旱变异株系。自然干旱条件下,对筛选出的3个抗旱变异株系进行了生理生化分析,结果表明,随自然干旱时间的延长,3个抗旱变异株系均表现出叶片相对含水量和叶绿素含量下降、SOD活性先升后降、丙二醛含量及游离脯氨酸含量上升等特征;不同株系间各指标的变化幅度存在差异,通过上述5项指标综合评价后初步认为,3个抗旱变异株系的抗旱性顺序为SP-64>SP-119>SP-17,与表征永久萎蔫系数反映的结果一致,表明筛选出的抗旱变异株系具有抗旱生理基础。以上结果说明,空间诱变能诱发多年生黑麦草抗旱性的变异;获得的抗旱变异株系为选育抗旱草坪草品种提供了新的种质资源。

Abstract: Three mutants with high drought tolerance (SP-64, SP-119, SP-17) have been screened from plants regenerated from the embryogenic calli of the Lolium perenne variety Derby, which were carried aboard by the Shenzhou No.7 spaceship. This paper presents the results of an evaluation of the physiological responses to drought treatment for these 3 mutants. As drought duration increased, the relative water content and chlorophyll content of the mutants decreased, whereas malondialdehyde (MDA) and soluble proline content increased. However, the 3 mutants’ superoxide dismutase (SOD) activities at first decreased and then increased. The variation ranges of indices were different for each mutation. A comprehensive analysis of these indices suggests that drought tolerance decreased in the order of SP-64, SP-119 and SP-17, which is consistent with the results of the apparent permanent wilting index (APWI). These results indicate that space mutation could induce the mutagenesis of drought resistance and so provide new germplasm resources for the breeding of turfgrass varieties.

彭丽梅, 曹丽, 韩蕾, 钱永强, 孙振元. 多年生黑麦草愈伤组织航天搭载后再生株系的抗旱性研究[J]. 草业学报, 2015, 24(1): 64-74.

PENG Limei, CAO Li, HAN Lei, QIAN Yongqiang, SUN Zhenyuan. Drought resistance analysis of regenerated plants from calli of Lolium perenne carried by the Shenzhou No.7 spaceship[J]. Acta Prataculturae Sinica, 2015, 24(1): 64-74.

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多年生黑麦草愈伤组织航天搭载后再生株系的抗旱性研究

Drought resistance analysis of regenerated plants from calli of Lolium perenne carried by the Shenzhou No.7 spaceship

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