欢迎访问《草业学报》官方网站,今天是 分享到:

草业学报 ›› 2015, Vol. 24 ›› Issue (3): 219-225.DOI: 10.11686/cyxb20150323

• 研究简报 • 上一篇    下一篇

柴胡中草药对奶牛瘤胃菌群多样性及纤维分解菌的影响

潘龙1,卜登攀1*,王加启1,程建波1,孙先枝1,王秀敏2,秦俊杰2,袁耀明3,张幸开3   

  1. 1.中国农业科学院北京畜牧兽医研究所,动物营养学国家重点实验室,北京 100193;
    2.北京生泰尔生物科技有限公司,北京 102206;
    3.上海光明荷斯坦牧业有限公司,上海 200436
  • 收稿日期:2014-02-28 修回日期:2014-04-21 出版日期:2015-03-20 发布日期:2015-03-20
  • 通讯作者: E-mail:burdenpan@gmail.com
  • 作者简介:潘龙(1988-),男,江苏邳州人,在读硕士。E-mail:panlong8809@163.com
  • 基金资助:
    “十二五”科技支撑计划(2012BAD12B02-5)和中国农业科学院科技创新工程(ASTIP-IAS07)资助。

Effects of radix bupleuri herbal supplementation on bacterial community diversity in the rumen of lactating dairy cows

PAN Long1, BU Dengpan1*, WANG Jiaqi1, CHENG Jianbo1, SUN Xianzhi1, WANG Xiumin2, QIN Junjie2, YUAN Yaoming3, ZHANG Xingkai3   

  1. 1.The College of Gardening and Horticulture, Yangtze University, Jingzhou 434025, China;
    2.Baokang Country Forestry Bureau, Baokang 441600, China;
    3.The College of Forestry and Horticulture, Hubei Minzu University, Enshi 445000, China
  • Received:2014-02-28 Revised:2014-04-21 Online:2015-03-20 Published:2015-03-20

摘要: 通过DGGE和RT-PCR技术研究日粮中添加不同剂量的柴胡中草药对奶牛瘤胃细菌多样性和主要纤维分解菌(琥珀酸丝状杆菌、黄色瘤胃球菌和白色瘤胃球菌)的影响。根据产奶量(37.5±1.8) kg/d、泌乳天数(75±15) d以及胎次(1.7±0.4)等相近的原则,将40头健康的中国荷斯坦泌乳奶牛随机分为4组(n=10),分别饲喂4种不同的处理日粮,即在基础日粮中分别添加0,0.25,0.50和1.00 g/kg的柴胡中草药(DM基础)。试验持续10周,并在第6周口腔采集瘤胃液,通过变性梯度凝胶电泳(DGGE)和实时定量PCR(RT-PCR)对瘤胃细菌进行分析。DGGE图谱显示,中草药添加组和空白对照组并没有显著的差异条带,但其指纹图谱相似性并不高,均低于0.54,且1.00 g/kg的添加量显著降低了瘤胃液细菌香农多样性指数,而0.50和1.00 g/kg的添加量则提高了菌群优势度指数(P<0.05);RT-PCR结果显示,柴胡中草药对奶牛瘤胃主要纤维分解菌并没有显著的影响。因此,柴胡中草药在一定程度上影响了瘤胃细菌的多样性,但差异并不显著,可能由于瘤胃微生物适应了柴胡中草药的添加。

Abstract: This experiment was conducted to investigate the effect of radix bupleuri (Bupleurum chinensis) herbal supplementation (RBH) on the diversity of cellulolytic bacteria including Fibrobacter fuccinogene, Ruminococcus albus and Ruminococcus flavefaciens in the rumen of lactating dairy cows. Forty Holstein cows were randomly assigned to 4 diet groups (n=10) according to milk yield (37.5±1.8) kg/d and lactation length (75±15) days. Four treatment diets consisted of 0, 0.25, 0.50 or 1.00 g/kg of RHB dry matter, respectively. The experiment lasted for 10 weeks; rumen fluid samples were collected using a stomach-tube during week 6 of the trial. The diversity of the bacterial community in the rumen was assessed using denaturing gradient gel electrophoresis (DGGE) and real-time quantitative polymerase chain reaction (RT-PCR) analysis. The DGGE analysis indicated that there were no differences among the treatment groups. Compared with the control group, the Shannon-Weiner index was significantly reduced (P<0.05) in the 1.0 g/kg RBH group, while the dominance index was significantly increased (P<0.05) in the 0.50 and 1.00 g/kg treatments. RT-PCR analysis also showed that RBH had no effect on cellulolytic bacteria. It was concluded that RBH supplementation had little effect on diversity of the bacterial community in the rumen of lactating dairy cows, possibly due to gradual adaptation to the RBH supplementation by rumen bacteria.