不同品种小麦抗氧化系统对水分胁迫的响应

doi:10.11686/cyxb2014342

摘要/Abstract

摘要： 以永良4号和西旱2号两小麦品种为实验材料,在水分胁迫和复水条件下,研究了两种小麦叶、鞘和茎中脯氨酸(Pro)、可溶性糖、丙二醛(MDA)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)等抗旱指标的变化,探讨了两小麦品种在水分胁迫和复水后的生理生化特征。结果表明,随水分胁迫程度和时间的增加,两小麦中渗透调节物质Pro含量和膜脂过氧化物MDA含量呈持续上升的趋势,而可溶性糖含量则先升后降,且永良4号中MDA含量增加幅度要高于西旱2号。抗氧化物酶SOD、CAT和POD活性在轻度水分胁迫下均有所上升,且西旱2号中抗氧化酶活性增加幅度均大于永良4,三者在中度水分胁迫下则表现出先升后降的趋势,可能是因为中度水分胁迫下抗氧化物酶丧失了活性。在复水处理中,以上各指标均有不同程度的恢复,且西旱2号的总体恢复效应要好于永良4号。在水分胁迫和复水处理下,两小麦3种器官中以上各指标变化幅度依次为叶>鞘>茎。以上结果说明西旱2号抗旱性强于永良4号,且3种器官对水分胁迫表现最为敏感的为叶,鞘次之,茎最不敏感。

Abstract: In this study, the concentration or activity changes of some drought indicators, including proline (Pro), soluble sugar, malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) and superoxidase (POD) were measured with two wheat varieties “Yongliang 4” and “Xihan 2” under water deficit stress. The contents of Pro and MDA increased and the soluble sugar content rose initially and then fell with increased degree and duration of water stress. The MDA content in “Yongliang 4” increased more than that in “Xihan 2”. The activities of SOD, CAT and POD increased under mild water stress, and activities of these enzymes in “Xihan 2” increased more than those in “Yongliang 4”. However, the activities of the three enzymes rose initially and then decreased under moderate water stress. All the indicators showed differing recovery patterns after rehydration, and compared with “Yongliang 4”, the overall drought resistance and recovery response of “Xihan 2” was better. The degree of change of the above stress indictors was ranked: leaf>sheath>stem, showing that the most sensitive organ to water stress was the leaf, and the least sensitive was the stem.

时振振, 李胜, 马绍英, 王雅梅, 苏李维, 唐斌, 赵生琴, 苏利荣. 不同品种小麦抗氧化系统对水分胁迫的响应[J]. 草业学报, 2015, 24(7): 68-78.

SHI Zhen-Zhen, LI Sheng, MA Shao-Ying, WANG Ya-Mei, SU Li-Wei, TANG Bin, ZHAO Sheng-Qin, SU Li-Rong. Response of the antioxidant system to water stress in different wheat varieties[J]. Acta Prataculturae Sinica, 2015, 24(7): 68-78.

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