干旱胁迫下硅对肥皂草抗氧化系统及膜质稳定性的影响

doi:10.11686/cyxb2016486

草业学报 ›› 2017, Vol. 26 ›› Issue (10): 77-86.DOI: 10.11686/cyxb2016486

干旱胁迫下硅对肥皂草抗氧化系统及膜质稳定性的影响

杨慧颖^{1, 2}, 邓雅楠², 许凌欣², 严俊鑫^{1, 2, *}

1.东北林业大学东北油田盐碱植被恢复与重建教育部重点实验室,黑龙江哈尔滨 150040;
2.东北林业大学园林学院,黑龙江哈尔滨 150040

收稿日期:2016-12-26 出版日期:2017-10-20 发布日期:2017-10-20
通讯作者: yanjunxin@163.com
作者简介:杨慧颖(1992-),女,河北秦皇岛人,硕士。E-mail:ylzwyhy@163.com
基金资助:
东北油田盐碱植被恢复与重建教育部重点实验室(东北林业大学)开放基金(SAVER1609)和中央高校基本科研业务费专项基金(2572016CA11) 资助

Effects of silicon on the antioxidant system and membrane stability of Saponaria officinalis under drought stress

YANG Hui-Ying^{1, 2}, DENG Ya-Nan², XU Ling-Xin², YAN Jun-Xin^{1, 2, *}

1.Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field, Ministry of Education, Northeast Forestry University, Harbin 150040, China;
2.The College of Landscape, Northeast Forestry University, Harbin 150040, China

Received:2016-12-26 Online:2017-10-20 Published:2017-10-20

摘要/Abstract

摘要： 以盆栽肥皂草为试验材料,通过对每kg干土施0.1,0.2,0.3,0.4 g SiO₂并设置正常水分、轻度胁迫、重度胁迫3种水分梯度,测定并分析胁迫期间肥皂草叶片保护酶活性、抗氧化剂含量、丙二醛含量和相对电导率的变化情况,以期探讨干旱胁迫下硅对抗氧化系统与膜质稳定性的影响。结果表明,随着干旱胁迫程度和天数的增加,肥皂草叶片SOD、POD、CAT活性和GSH、ASA含量呈现先上升后下降趋势,而丙二醛含量与叶片相对电导率呈现增加趋势。与CK相比,施一定量的硅能使干旱条件下肥皂草SOD、POD、CAT活性最高增加30%以上,丙二醛含量和相对电导率降低达25%以上,并稳定了GSH、ASA含量,说明硅能有效缓解干旱对肥皂草细胞膜的破坏,提高肥皂草抗氧化系统的防御能力,保护其正常生理机能不被破坏。随着硅浓度的增加,肥皂草抗旱性逐渐增强并趋于平稳,最适宜硅肥浓度为每kg干土SiO₂含量0.3~0.4 g。

Abstract: The aim of this study was to determine the effect of silicon on the antioxidant system and membrane stability of Saponaria officinalis under drought stress. Silicon was added to soil at four concentrations (0.1, 0.2, 0.3, 0.4 g/kg SiO₂) and the soil moisture content was adjusted to impose normal conditions, mild drought stress, and severe drought stress on S. officinalis. The protective enzyme activity, antioxidant content, malondialdehyde (MDA) content, and relative electrical conductivity of the leaves of S. officinalis were determined. The results showed that with increasing severity and longer duration of drought stress, the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), and the contents of reduced glutathione (GSH) and ASA (ascorbic acid) in the leaves of S. officinalis first increased and then decreased, and the MDA content and relative electric conductivity increased. Compared with the control, the treatments with silicon added to soil showed higher activities of SOD, POD, CAT (>30% higher) under drought stress, and lower MDA content and relative electrical conductivity (>25% lower), resulting in more stable GSH and ASA contents. These results indicated that silicon can effectively alleviate damage to S. officinalis cell membranes under drought stress by improving the performance of the antioxidant system. As the concentration of silicon increased, the drought resistance of S. officinalis increased gradually and then stabilized. The optimal concentration of SiO₂ in soil was 0.3-0.4 g/kg.

杨慧颖, 邓雅楠, 许凌欣, 严俊鑫. 干旱胁迫下硅对肥皂草抗氧化系统及膜质稳定性的影响[J]. 草业学报, 2017, 26(10): 77-86.

YANG Hui-Ying, DENG Ya-Nan, XU Ling-Xin, YAN Jun-Xin. Effects of silicon on the antioxidant system and membrane stability of Saponaria officinalis under drought stress[J]. Acta Prataculturae Sinica, 2017, 26(10): 77-86.

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干旱胁迫下硅对肥皂草抗氧化系统及膜质稳定性的影响

Effects of silicon on the antioxidant system and membrane stability of Saponaria officinalis under drought stress

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