甜高粱青贮有氧暴露的稳定性及微生物变化的研究

doi:10.11686/cyxb2016304

摘要/Abstract

摘要： 为了更好地开发利用甜高粱青贮饲料,本研究以高糖甜高粱青贮(HSS)、低糖甜高粱青贮(LSS)和玉米青贮(MZS)为材料,研究了在有氧暴露0、5、10、15、20和25 d时干物质、水溶性碳水化合物(WSC)、pH、温度和微生物数量的变化。结果表明,在有氧暴露前期(0~5 d),与LSS和MZS比较,HSS具有较低的pH值,较高的乳酸菌含量和较高的温度;在有氧暴露的后期(15~25 d),HSS酵母菌、霉菌和好氧菌数量均显著大于LSS和MZS (P<0.05),LSS的温度和pH值显著低于HSS和MZS(P<0.05);在整个有氧过程中,WSC含量在HSS中最高,干物质含量在LSS中最高(P<0.05)。可见,在有氧暴露环境下,LSS有氧稳定性最好,酵母菌、霉菌和好氧细菌数量增加最少;HSS有氧稳定性最差,酵母菌、霉菌和好氧细菌数量增加最多;MZS有氧稳定性和好氧性微生物数量居中。本研究结果提示,HSS在有氧暴露环境下更容易发生二次发酵,因此,有必要在青贮中使用添加剂,以保证HSS在使用过程的营养品质。

Abstract: In order to promote the exploitation and utilization of sweet sorghum silage, three kinds of silages (high sugar sweet sorghum silage, HSS; low sugar sweet silage, LSS; maize silage, MZS) were used to analyze the changes in dry matter (DM), water soluble carbohydrates (WSC), pH, temperature and microbial populations during aerobic exposure over 0, 5, 10, 15, 20 and 25 days. The results showed that HSS had a lower pH and higher LAB (lactic acid bacteria) population and higher temperature compared with LSS and MZS (P<0.05) in the early stage of aerobic exposure (0-5 d). The numbers of yeast, mould and aerobic bacteria in HSS were higher than that in LSS and MZS (P<0.05), while temperature and pH were lower in LSS than those in HSS and MZS (P<0.05) in the latter stages of aerobic exposure (15-25 d). HSS had the highest WSC and LSS had the lowest dry matter content (P<0.05) over all exposure periods. Overall, LSS had the greatest aerobic stability with less yeast, mould and aerobic bacteria which were highest in HSS which had the lowest aerobic stability; bacteria numbers and aerobic stability were intermediate in in MZS. The results of this study suggest that it is necessary to use additives when making HSS to assure quality because it is more prone to secondary fermentation under aerobic exposure.

周斐然, 张苏江, 王明, 肖丹, 郭雪峰. 甜高粱青贮有氧暴露的稳定性及微生物变化的研究[J]. 草业学报, 2017, 26(4): 106-112.

ZHOU Fei-Ran, ZHANG Su-Jiang, WANG Ming, XIAO Dan, GUO Xue-Feng. Aerobic stability and microbial populations in sweet sorghum silages during aerobic exposure[J]. Acta Prataculturae Sinica, 2017, 26(4): 106-112.

参考文献

[1] Corredor D Y, Salazar J M, Hohn K L, et al . Evaluation and characterization of forage sorghum as feedstock for fermentable sugar production. Applied Biochemistry & Biotechnology, 2009, 158(1): 164-179.
[2] Xiao D, Zhang S J, Chen L Q, et al . Prospect analysis on the development of sweet sorghum as forage resources in Southern Xinjiang. Grass-Feeding Livestock, 2014, (6): 22-27.
肖丹, 张苏江, 陈立强, 等. 甜高粱饲料在南疆粗饲料资源开发中的前景分析. 草食家畜, 2014, (6): 22-27.
[3] Zhang S J, Chaudhry A S, Ramdani D, et al . Chemical composition and in vitro fermentation characteristics of high sugar forage sorghum as an alternative to forage maize for silage making in Tarim Basin, China. Journal of Integrative Agriculture, 2016, 15(1): 175-182.
[4] Marco O N D, Ressia M A, Arias S, et al . Digestibility of forage silages from grain, sweet and bmr sorghum types: Comparison of in vivo , in situ and in vitro data. Animal Feed Science & Technology, 2009, 153(3): 161-168.
[5] Amer S, Seguin P, Mustafa A F. Short communication: Effects of feeding sweet sorghum silage on milk production of lactating dairy cows. Journal of Dairy Science, 2012, 95(2): 859-863.
[6] Guo X S, Zhou H, Yu Z. Effects of different additives on the aerobic stability of silage. China Dairy Cattle, 2006, (9): 18-21.
郭旭生, 周禾, 玉柱. 不同添加剂对青贮饲料有氧稳定性的影响. 中国奶牛, 2006, (9): 18-21.
[7] Zhou D B. Research, development and actuality for corn stover silage. Amino Acids and Biotic Resources, 2004, 26(2): 32-34.
周德宝. 青贮饲料的研究、发展及现状. 氨基酸和生物资源, 2004, 26(2): 32-34.
[8] Ma X G, Liu J J, Zheng Z H, et al . Effects of acetic and lactic acid in corn stover silage on aerobic stability and methane yield rate. Journal of China Agricultural University, 2015, 20(1): 44-52.
马旭光, 刘晶晶, 郑泽慧, 等. 乙酸和乳酸对玉米秸秆青贮料有氧稳定性和甲烷产率的影响. 中国农业大学学报, 2015, 20(1): 44-52.
[9] Guan W T, Ashbell G, Hen Y, et al . Effects of microbial inoculants applied to sorghum at ensiling on silage characteristics and aerobic stability. Scientia Agricultura Sinica, 2002, 35(11): 1401-1405.
管武太, Ashbell G, Hen Y, 等. 微生物添加剂对青贮高粱发酵品质和稳定性的影响. 中国农业科学, 2002, 35(11): 1401-1405.
[10] Wang Y, Yuan X J, Guo G, et al . Fermentation and aerobic stability of mixed ration forages in Tibet. Acta Prataculturae Sinica, 2014, 23(6): 95-102.
王勇, 原现军, 郭刚, 等. 西藏不同饲草全混合日粮发酵品质和有氧稳定性的研究. 草业学报, 2014, 23(6): 95-102.
[11] Xu Q F, Yu Z, Li Z Q, et al . A study on aerobic stability of alfalfa and corn silage. Acta Agrestia Sinica, 2007, 15(6): 519-524.
许庆方, 玉柱, 李志强, 等. 苜蓿、玉米青贮饲料有氧稳定性研究. 草地学报, 2007, 15(6): 519-524.
[12] Zhang S J, Osman A, Xue X Z, et al . Quality analysis on different sweet sorghum silages in Southern Xinjiang compared with a corn silage. Acta Prataculturae Sinica, 2014, 23(3): 232-240.
张苏江, 艾买尔江·吾斯曼, 薛兴中, 等. 南疆玉米和不同糖分甜高粱的青贮品质分析. 草业学报, 2014, 23(3): 232-240.
[13] Hu W, Schmidt R J, Mcdonell E E, et al . The effect of Lactobacillus buchneri 40788 or Lactobacillus plantarum MTD-1 on the fermentation and aerobic stability of corn silages ensiled at two dry matter contents. Journal of Dairy Science, 2009, 92(8): 3907-3914.
[14] Yu R H, Zhao L H, Mo F, et al . Determination of water soluble carbohydrate in corn straw silage. Feed Industry, 2003, 24(9): 38-39.
余汝华, 赵丽华, 莫放, 等. 玉米秸秆青贮饲料中水溶性碳水化合物测定方法研究. 饲料工业, 2003, 24(9): 38-39.
[15] Zhang G N, Zhang Q. Study on microbial culture method in silage. Feed Industry, 2010, 31(7): 19-22.
张高娜, 张骞. 青贮饲料中微生物培养方法的研究. 饲料工业, 2010, 31(7): 19-22.
[16] Ashbell G, Pahlow G, Barbara D, et al . Dynamics of orange peel fermentation during ensilage. Journal of Applied Microbiology, 1987, 63(4): 275-279.
[17] Yang Y G, Zhang Y L, Du X, et al . Study on the major microorganism changes during the silage processing of two kinds of corn silage. Chinese Journal of Animal & Veterinary Sciences, 2012, 43(3): 397-403.
杨云贵, 张越利, 杜欣, 等. 2种玉米青贮饲料青贮过程中主要微生物的变化规律研究. 畜牧兽医学报, 2012, 43(3): 397-403.
[18] Wang B P, Dong X Y, Dong K H, et al . Effects of organic acid on the aerobic stability of whole-crop corn silage. Acta Agrestia Sinica, 2013, 21(5): 991-997.
王保平, 董晓燕, 董宽虎, 等. 有机酸对全株玉米青贮有氧稳定性的影响. 草地学报, 2013, 21(5): 991-997.
[19] Qin L G. Study on aerobic stability of corn silage with different additives. China Dairy, 2010, (8): 40-42.
秦立刚. 不同添加剂对青贮玉米的有氧稳定性研究. 中国乳业, 2010, (8): 40-42.
[20] Stokes M R. Effects of an enzyme mixture, an inoculant, and their interaction on silage fermentation and dairy production. Journal of Dairy Science, 1992, 75(3): 764-773.
[21] Guo H M, Zhu W, Zhang Y, et al . Effect of additives on the quality of asparagus officinalis stem leaf silage. Acta Prataculturae Sinica, 2016, 25(5): 134-140.
郭海明, 朱雯, 张勇, 等. 青贮添加剂对芦笋茎叶青贮品质的影响. 草业学报, 2016, 25(5): 134-140.
[22] Spoelstra S F, Driehuis F, Elferink S J. Anaerobic lactic acid degradation during ensilage of whole crop maize inoculated with. Journal of Applied Microbiology, 1999, 87(4): 583-593.
[23] Ashbell G, Lisker N. Aerobic deterioration in maize silage stored in a bunker silo under farm conditions in a subtropical climate. Journal of the Science of Food & Agriculture, 1987, 45(4): 307-315.
[24] Kung L, Taylor C C, Lynch M P, et al . The effect of treating alfalfa with Lactobacillus buchneri , 40788 on silage fermentation, aerobic stability, and nutritive value for lactating dairy cows. Journal of Dairy Science, 2002, 85(7): 1793-1800.
[25] Weinberg Z G, Ashbell G, Hen Y, et al . The effect of applying lactic acid bacteria at ensiling on the aerobic stability of silages. Journal of Applied Microbiology, 2008, 75(6): 512-518.
[26] Taylor C C, Kung L. The effect of Lactobacillus buchneri , 40788 on the fermentation and aerobic stability of sigh moisture corn in laboratory silos. Journal of Dairy Science, 2002, 85(6): 1526-1532.