外源H2O2处理对燕麦种子活力的影响

doi:10.11686/cyxb2017183

草业学报 ›› 2018, Vol. 27 ›› Issue (3): 201-207.DOI: 10.11686/cyxb2017183

• 研究简报 • 上一篇

外源H₂O₂处理对燕麦种子活力的影响

夏方山^1,2*,*, 王婷婷^1*,*, 董秋丽³, 毛培胜², 朱慧森¹, 杜利霞¹, 佟莉蓉¹

1.山西农业大学动物科技学院,山西太谷 030801;
2.中国农业大学动物科技学院,草业科学北京市重点实验室,北京 100193;
3.山西农业大学林学院,山西太谷 030801;

收稿日期:2017-04-11 修回日期:2017-07-25 出版日期:2018-03-20 发布日期:2018-03-20
作者简介:夏方山(1983-),男,山东安丘人,讲师,博士。E-mail: dqlxfs8583@163.com; 王婷婷(1996-),女,山西祁县人,在读本科。E-mail: 17835423011@163.com.
基金资助:
山西省应用基础研究项目青年科技研究基金(201701D221154)和国家自然基金面上项目(31572454)资助

Effects of exogenous H₂O₂ treatment on the vigor of oat seeds

XIA Fang-shan^1,2,**, WANG Ting-ting^1,**, DONG Qiu-li³, MAO Pei-sheng², ZHU Hui-sen¹, DU Li-xia¹, TONG Li-rong¹

1.College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China;
2.College of Animal Science and Technology, China Agricultural University, Beijing Key Laboratory of Grassland Science, Beijing 100193, China;
3.College of Forestry, Shanxi Agricultural University, Taigu 030801, China;

Received:2017-04-11 Revised:2017-07-25 Online:2018-03-20 Published:2018-03-20

摘要/Abstract

摘要： 试验以燕麦种子为材料,通过不同浓度H₂O₂(0,0.24,0.48,0.96,1.92和3.84 mol·L^-1)处理0(CK),3,6,9和12 h后,分析其发芽率(G_p),发芽指数(G_i),平均发芽时间(MGT)及幼苗活力指数(SVI)的变化,以探讨外源H₂O₂处理对种子活力的影响。结果表明,高浓度的H₂O₂处理降低了燕麦种子的G_p、G_i及SVI,且提高了其MGT。H₂O₂浓度、处理时间及两者之间的交互作用对燕麦种子G_p、G_i、MGT和SVI的影响差异极显著。浓度为3.84 mol·L^-1的H₂O₂处理12 h后,燕麦种子G_p、G_i和SVI最小,而其MGT则最大。这表明H₂O₂浓度越高,处理时间越长,燕麦种子活力的丧失就越严重。因此,高浓度H₂O₂处理造成的脂质过氧化损伤可能是导致种子活力丧失的主要原因,在农牧业生产中外源H₂O₂预处理的应用要谨慎。

Abstract: In order to determine the effect of exogenous H₂O₂ on oat (Avena sativa) seed vigor during the germination process, oat seeds were pre-treated with 5 different concentrations of H₂O₂ (0, 0.24, 0.48, 0.96, 1.92, 3.84 mol·L^-1) for 0 (CK), 3, 6, 9 and 12 h. The performance indices measured included germination percentage (G_p), germination index (G_i), mean germination time (MGT) and seedling vigor index (SVI). The results showed that with high H₂O₂ concentrations the G_p, G_i and SVI of oat seeds decreased but their MGT increased. The effects of H₂O₂ on changes in G_p, G_i, MGT and SVI were significantly related to treatment concentrations, times and their interactions. The G_p, G_i and SVI of oat seeds reached their minimum level and MGT its maximum level after treatment with H₂O₂ concentration of 3.84 mol·L^-1 for 12 h. These results indicate that the higher the H₂O₂ concentration and the longer of treatment time, the more serious the loss of oat seed vigor. The lipid peroxidation damage caused by high concentration H₂O₂ treatments might be a major reason for the loss of seed vigor. This study suggests that the application of H₂O₂ in seed pre-treatment should be prudently undertaken.

夏方山, 王婷婷, 董秋丽, 毛培胜, 朱慧森, 杜利霞, 佟莉蓉. 外源H₂O₂处理对燕麦种子活力的影响[J]. 草业学报, 2018, 27(3): 201-207.

XIA Fang-shan, WANG Ting-ting, DONG Qiu-li, MAO Pei-sheng, ZHU Hui-sen, DU Li-xia, TONG Li-rong. Effects of exogenous H₂O₂ treatment on the vigor of oat seeds[J]. Acta Prataculturae Sinica, 2018, 27(3): 201-207.

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外源H₂O₂处理对燕麦种子活力的影响

Effects of exogenous H₂O₂ treatment on the vigor of oat seeds

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