高丹草种子半透特性的研究

doi:10.11686/cyxb2017218

草业学报 ›› 2018, Vol. 27 ›› Issue (5): 162-169.DOI: 10.11686/cyxb2017218

高丹草种子半透特性的研究

孙秋瑾, 吕燕燕, 韩云华, 王彦荣^*

草地农业生态系统国家重点实验室,兰州大学草地农业科技学院,甘肃兰州 730020

收稿日期:2017-05-08 修回日期:2017-06-01 出版日期:2018-05-20 发布日期:2018-05-20
通讯作者: * E-mail: yrwang@lzu.edu.cn
作者简介:孙秋瑾(1991-),女,甘肃武威人,在读硕士。E-mail: sunqj15@lzu.edu.cn
基金资助:
国家重点基础研究发展计划(973)项目(2014CB138704)资助

Study of the semipermeable characteristics of Sorghum bicolor×Sorghum sudanense seeds

SUN Qiu-jin, LÜ Yan-yan, HAN Yun-hua, WANG Yan-rong^*

State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China

Received:2017-05-08 Revised:2017-06-01 Online:2018-05-20 Published:2018-05-20
Contact: * E-mail: yrwang@lzu.edu.cn

摘要/Abstract

摘要： 以3个不同品种的高丹草种子为材料,通过测定吸水率、电导率,四唑盐染色和荧光染色剂示踪等方法,研究了高丹草种子对物质渗漏的半透性差异,探讨了种子半透层的存在对电导率法测定种子活力的影响,为种子质量评价提供科学依据。结果表明,高丹草种皮对水分通透性良好,对四唑盐和电解质的通透性较差,完整高丹草种子的种胚四唑盐重度染色率较低[分别为5%(冀草2号)、0%(冀草8号)和2%(冀草008号)],不同发芽率种子电导率值无显著差异(P>0.05);刺破种皮后,种胚四唑盐重度染色率显著升高(3个品种均达100%),电导率与发芽率呈极显著负相关(P<0.01)。3种高丹草种子对荧光染色剂的通透性不同,其中冀草8号和冀草008号种皮限制荧光素的渗入。采用硝酸镧示踪结合X射线能量色散分析证明高丹草种胚包被物中存在半透层,该组织是引起种皮半透性差异的因素之一,其对电解质渗漏的阻碍,限制了电导率法对高丹草种子活力评价的应用。

关键词: 高丹草种子, 半透性, 半透层, 种子活力

Abstract: This study examines seed semipermeable properties and solutes leakage, investigating the testing of seed vigor by exploring the relationship between the presence of a semipermeable layer and electrical conductivity.In order to provide a scientific basis for seed quality evaluation, three Sorghum bicolor×Sorghum sudanense varieties were tested using measurements of water imbibition rate, electrical conductivity, tetrazolium salt uptake, and the fluorescent tracers technique. The results showed that the seed coat of Sorghum bicolor×Sorghum sudanense readily imbibed water, while showing poor permeability for tetrazolium salts and electrolytes. Intact seeds exhibited less strong staining with tetrazolium salt (5%, 0%, and 2% for Jicao No.2, Jicao No.8 and Jicao No.008, respectively) and there was no significant difference in the electrical conductivity of seeds with different germination rates (P>0.05). After puncturing, all seeds were strongly stained (100%) and electrical conductivity was significantly negatively correlated with germination rate (P<0.01). The seed coat permeability of the three Sorghum bicolor×Sorghum sudanense varieties to fluorescent tracers were different. Jicao No.8 and Jicao No.008 seed coats were not permeable to fluorescein. Lanthanum nitrate tracer combined with X-ray dispersive energy analysis revealed that a semipermeable layer was presented in the seed coverings of Sorghum bicolor×Sorghum sudanense. This is one of the main causes of differences in seed coat semipermeability, and its inhibition of electrolytes leakage resulted in the failure of electroconductivity tests to assess seed vigor.

Key words: Sorghum bicolor×Sorghum sudanense seed, semipermeability, semipermeable layer, seed vigor

孙秋瑾, 吕燕燕, 韩云华, 王彦荣. 高丹草种子半透特性的研究[J]. 草业学报, 2018, 27(5): 162-169.

SUN Qiu-jin, LÜ Yan-yan, HAN Yun-hua, WANG Yan-rong. Study of the semipermeable characteristics of Sorghum bicolor×Sorghum sudanense seeds[J]. Acta Prataculturae Sinica, 2018, 27(5): 162-169.

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高丹草种子半透特性的研究

Study of the semipermeable characteristics of Sorghum bicolor×Sorghum sudanense seeds

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