四倍体马铃薯SRAP分子遗传连锁图谱的构建

doi:10.11686/cyxb2019230

草业学报 ›› 2019, Vol. 28 ›› Issue (8): 190-199.DOI: 10.11686/cyxb2019230

四倍体马铃薯SRAP分子遗传连锁图谱的构建

张明飞, 于卓, 于肖夏^*, 李景伟, 马艳红, 吴国芳, 王颖

内蒙古农业大学农学院,内蒙古呼和浩特 010019

收稿日期:2019-04-09 出版日期:2019-08-20 发布日期:2019-08-20
通讯作者: *,E-mail: yuxiaoxia1985@sina.com
作者简介:张明飞(1990-),男,内蒙古赤峰市人,在读博士。E-mail: zhangmingfei0207@126.com
基金资助:
内蒙古自治区科技重大专项(ZDZX2018019),内蒙古科技计划重点项目(2016ZD),内蒙古农业大学科技成果转化专项资金动植物品种培育项目(YZGC2017006)和内蒙古自治区高等学校青年科技英才支持计划(NJYT-18-B04)资助

Construction of a genetic linkage map for tetraploid potato based on SRAP markers

ZHANG Ming-fei, YU Zhuo, YU Xiao-xia^*, LI Jing-wei, MA Yan-hong, WU Guo-fang, WANG Ying

Inner Mongolia Agricultural University Agricultural College, Hohhot 010019, China

Received:2019-04-09 Online:2019-08-20 Published:2019-08-20
Contact: *,E-mail: yuxiaoxia1985@sina.com

摘要/Abstract

摘要： 为了给后期马铃薯块茎高淀粉、高干物质及产量等重要数量性状基因座(QTL)定位和分子标记辅助育种奠定基础,以四倍体马铃薯YSP-4×MIN-021杂种F₁的182个分离单株为作图群体,利用相关序列扩增多态性(SRAP)分子标记进行了遗传图谱构建研究。试验从357对SRAP引物中筛选出适宜引物36对。用这些引物对马铃薯杂种F₁的182个分离单株及其亲本的基因组DNA进行PCR扩增得到598个SRAP标记。卡方检验显示偏分离标记数为127个,其中母本连锁群59个,父本连锁群68个,偏分离比率均小于30%,符合遗传作图的要求。用软件Join Map 4.0建立了2张四倍体马铃薯双亲的SRAP遗传图谱。母本YSP-4的连锁群总长度为1572.2 cM,标记数目为274个,平均间距为5.74 cM,12个连锁群的长度范围为14.03~214.44 cM;父本MIN-021的连锁群总长度为1932.23 cM,标记数目为324个,平均间距为5.96 cM,各连锁群的长度范围为93.65~242.06 cM。

关键词: 四倍体马铃薯, SRAP标记, 遗传连锁图谱

Abstract: This research aimed to lay a foundation for molecular assisted breeding of potato through location of quantitative trait loci (QTL) for important traits such as high starch content, high dry matter content and yield of potato tubers. The mapping population comprised 182 F₁ genotypes from a cross of YSP-4×MIN-021 tetraploid hybrid potato lines. We constructed biparental genetic linkage maps based on sequence related amplified polymorphism (SRAP) molecular makers. A total of 36 out of 357 suitable SRAP primers were selected, the genomic DNA of the 182 F₁ genotypes and their parents was amplified, and a total of 598 SRAP markers was obtained. The chi-square test showed that there were 127 distorted markers, of which 59 were in the maternal linkage group, 68 were in the paternal linkage group, and the segregation distortion ratio was less than 30%, which conformed to the requirements for genetic mapping. The two parental SRAP genetic maps of tetraploid potato were established using the software package Join Map 4.0. The maternal map included 274 markers, had a total length of 1572.2 cM with an average interval of 5.74 cM, and the length of the linkage groups ranged from 14.03 cM to 214.44 cM. The paternal map included 324 markers, had a total length of 1932.23 cM with an average interval of 5.96 cM, and the length of the linkage groups ranged from 93.65 cM to 242.06 cM.

Key words: tetraploid potato, SRAP markers, genetic linkage map

张明飞, 于卓, 于肖夏, 李景伟, 马艳红, 吴国芳, 王颖. 四倍体马铃薯SRAP分子遗传连锁图谱的构建[J]. 草业学报, 2019, 28(8): 190-199.

ZHANG Ming-fei, YU Zhuo, YU Xiao-xia, LI Jing-wei, MA Yan-hong, WU Guo-fang, WANG Ying. Construction of a genetic linkage map for tetraploid potato based on SRAP markers[J]. Acta Prataculturae Sinica, 2019, 28(8): 190-199.

参考文献

[1] Lü J Z, Ran H, Jiang J C.Potato nutrition and health value. Food and Nutrition in China, 2009, 15(3): 51-52.
吕巨智, 染和, 姜建初. 马铃薯的营养成分及保健价值. 中国食物与营养, 2009, 15(3): 51-52.
[2] Lu X P.Strategy of potato as staple food: Significance, bottlenecks and policy suggestions. Journal of Huazhong Agricultural University, 2015, 35(3): 1-7.
卢肖平. 马铃薯主粮化战略的意义、瓶颈与政策建议. 华中农业大学学报, 2015, 35(3): 1-7.
[3] Zhang M F, Yu Z, Yu X X, et al. Main agronomic characters nutritional quality, cytology and SSR analysis of the seven superior new strains of potato. Acta Botanica Boreali-Occidentalia Sinica, 2018, 38(4): 62-71.
张明飞, 于卓, 于肖夏, 等. 7个马铃薯新品系的主要农艺性状与营养品质及其细胞学和SSR分析. 西北植物学报, 2018, 38(4): 62-71.
[4] Li G, Quiros C F.Sequence-related amplified polymorphism(SRAP), a new marker system based on a simple PCR reaction: Its application to mapping and gene tagging in Brassica. Theoretical and Applied Genetics, 2001, 103(2/3): 455-461.
[5] Lu C, Zhang M D, He Y S, et al. Application of SRAP technology in plant genetics and breeding. Modern Agricultural Science and Technology, 2014, 43(16): 18-19, 24.
卢超, 张美德, 何银生, 等. SRAP技术在植物遗传育种中的应用. 现代农业科技, 2014, 43(16): 18-19, 24.
[6] Lei J, Liu J.Identification of a SRAP marker linked to bacterial wilt resistance in diploid potato. Chinese Potato Journal, 2006, 20(3): 150-153.
雷剑, 柳俊. 一个与马铃薯青枯病抗性连锁的SRAP标记筛选. 中国马铃薯, 2006, 20(3): 150-153.
[7] Jiang S K, Ma H, Liu J, et al. Genetic diversity in maize inbred lines revealed by SRAPs marker. Molecular Plant Breeding, 2007, 5(3): 412-416.
姜树坤, 马慧, 刘君, 等. 利用SRAP标记分析玉米遗传多样性. 分子植物育种, 2007, 5(3): 412-416.
[8] Wang F T, Lin R M, Ouyang H Y, et al. The application of SRAP-PCR in genetic diversity study of Henan wheat cultivars. Journal of Plant Genetic Resources, 2009, 10(4): 517-521.
王凤涛, 蔺瑞明, 欧阳宏雨, 等. 利用SRAP标记分析河南小麦栽培品种的遗传多样性. 植物遗传资源学报, 2009, 10(4): 517-521.
[9] Wang Z W, Wei L M, Xie X M, et al. Establishment of cotton genetic map and QTL analysis of fiber quality by using AFLP. Jiangsu Agricultural Sciences, 2015, 43(7): 71-74.
王志伟, 魏利民, 谢晓美, 等.利用AFLP构建棉花遗传图谱及纤维品质性状QTL分析. 江苏农业科学, 2015, 43(7): 71-74.
[10] Yang D S, Yu Z, Yu X X, et al. Construction of the genetic linkage map of tetraploid hybrid crested wheatgrass based on SRAP and SSR markers. Journal of Triticeae Crops, 2018, 38(8): 941-948.
杨东升, 于卓, 于肖夏, 等. 四倍体杂交冰草SRAP和SSR分子标记遗传连锁图谱构建. 麦类作物学报, 2018, 38(8): 941-948.
[11] Hans V O, Andrzejewski S, Bakker E, et al. Construction of a 10,000-marker ultradense genetic recombination map of potato: Providing a framework for accelerated gene isolation and a genomewide physical map. Genetics, 2006, 173(2): 1075-1087.
[12] Bradshaw J E, Hackett C A, Pande B, et al. QTL mapping of yield, agronomic and quality traits in tetraploid potato (Solanum tuberosum subsp, tuberosum). Theoretical and Applied Genetics, 2008, 116(2): 193-211.
[13] Jin L P, Liu J, Jong H D, et al. Construction of a molecular linkage map in Solanum tuberosum L. Acta Horticulturae Sinica, 2007, 34(2): 397-402.
金黎平, 刘杰, Jong H D, 等. 二倍体马铃薯分子连锁图谱的构建. 园艺学报, 2007, 34(2): 397-402.
[14] Cui K S.Construction of high-density molecular genetic linkage maps and mapping QTLs for important traits such as anthocyanin content in colored potato. Hohhot: Inner Mongolia Agricultural University, 2015.
崔阔澍. 彩色马铃薯高密度分子遗传连锁图谱构建及花青素等重要性状QTL定位. 呼和浩特: 内蒙古农业大学, 2015.
[15] Pezzotti G, Yamada K.Genetic diversity of oilseed Brassica napus inbred lines based on sequence-related amplified polymorphism and its relation to hybrid performance. Plant Breeding, 2010, 120(5): 411-415.
[16] Lin Z X, He D H, Zhang X L, et al. Linkage map construction and mapping QTL for cotton fibre quality using SRAP, SSR and RAPD. Plant Breeding, 2008, 124(2): 180-187.
[17] Yu Z, Xie R, Yu X X, et al. SSR analysis of sorghum-sudangrass new strains with low hydrocyanic acid content. Acta Prataculturae Sinica, 2014, 23(1): 223-228.
于卓, 谢锐, 于肖夏, 等. 低氢氰酸含量高丹草新品系及其亲本的SSR分析. 草业学报, 2014, 23(1): 223-228.
[18] Nei M.Analysis of gene diversity in subdivided populations. Proceedings of the National Academy of Sciences of the USA, 1973, 70(12): 3321-3323.
[19] Song X L, Sun X Z, Zhang T Z.Segregation distortion and its effect on genetic mapping in plants. Journal of Agricultural Biotechnology, 2006, 14(2): 286-292.
宋宪亮, 孙学振, 张天真. 偏分离及对植物遗传作图的影响. 农业生物技术学报, 2006, 14(2): 286-292.
[20] Charlesworth B.Driving genes and chromosomes. Nature, 1988, 332: 394-395.
[21] Nishio S, Terakami S, Matsumoto T, et al. Identification of QTLs for agronomic traits in the Japanese chestnut (Castanea crenata Sieb. et Zucc.) breeding. The Horticulture Journal, 2018, 87(1): 43-54.
[22] Knox M R, Ellis T H.Excess heterozygosity contributes to genetic map expansion in pea recombinant inbred populations. Genetics, 2002, 162(2): 861-873.
[23] Tulsieram L K, Glaubitz J C, Kiss G, et al. Single tree genetic linkage mapping in conifers using haploid DNA from megagametophytes. Nature Biotechnology, 1992, 10(6): 686-690.
[24] Xu S J, Singh R J, Hymowitz T.Establishment of a cytogenetic map of soybean: Progress and prospective. Soybean Genetics Newsletter, 1997, 24: 121-122.
[25] Peng J, Cui C J, Liang G J, et al. Analysis of the genetic composition in Saccharina japonica and Undaria pinnatifida based on SSR markers. Biotechnology Bulletin, 2018, 34(3): 156-162.
彭捷, 崔翠菊, 梁广津, 等. 基于SSR标记的海带、裙带菜的遗传构成分析. 生物技术通报, 2018, 34(3): 156-162.
[26] Zhao N, Yuan X L, Miao F J, et al. Development of SSR molecular markers based on transcriptome data of Pinus kesiya var. langbianensis. Biotechnology Bulletin, 2017, 33(5): 71-77.
赵能, 原晓龙, 缪福俊, 等. 思茅松转录组SSR分析及标记开发. 生物技术通报, 2017, 33(5): 71-77.
[27] Wang X W, Lou P, He H J, et al. Construction of an AFLP-based genetic linkage map using a doubled-haploid (DH) population derived from a cross between Chinese kale and broccoli. Acta Horticulturae Sinica, 2005, 32(1): 30-34.
王晓武, 娄平, 何杭军, 等. 利用芥蓝×青花菜DH群体构建AFLP连锁图谱. 园艺学报, 2005, 32(1): 30-34.
[28] Bradshaw J B H, Villar M, Watson B D, et al. Molecular genetics of growth and development in Populus. Ⅲ. A genetic linkage map of a hybrid poplar composed of RFLP, STS and RAPD markers. Theoretical and Applied Genetics, 1994, 89(2/3): 167-178.
[29] Tang L, Jin M Y, Huang L K, et al. Enhancement of the genetic linkage map density of tetraploid based on SSR markers. Scientia Agricultura Sinica, 2018, 51(5): 991-998.
唐露, 金梦雅, 黄琳凯, 等. 基于SSR标记的四倍体鸭茅遗传图谱加密. 中国农业科学, 2018, 51(5): 991-998.
[30] Xu Y B, Zhu L H.Molecular quantitative genetics. Beijing: China Agriculture Press, 1994: 81-83.
徐云碧, 朱立煌. 分子数量遗传学. 北京: 中国农业出版社, 1994: 81-83.

四倍体马铃薯SRAP分子遗传连锁图谱的构建

Construction of a genetic linkage map for tetraploid potato based on SRAP markers

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 6

编辑推荐

Metrics

[1]	蒲小剑,田久胜,田新会,杜文华. 红三叶遗传图谱构建及抗白粉病基因QTL定位[J]. 草业学报, 2018, 27(4): 79-88.
[2]	姜志艳, 于肖夏, 于卓, 张志成, 石悦, 姜超. 利用SSR分子标记构建四倍体杂交冰草的遗传连锁图谱[J]. 草业学报, 2016, 25(6): 94-101.
[3]	梁小玉, 季杨, 白史且, 黄琳凯, 张新全. 基于SRAP分子标记构建的菊苣遗传连锁图谱[J]. 草业学报, 2015, 24(5): 153-158.
[4]	康俊梅,张铁军,王梦颖,张怡,杨青川. 紫花苜蓿QTL与全基因组选择研究进展及其应用[J]. 草业学报, 2014, 23(6): 304-312.
[5]	薛丹丹,郭海林,郑轶琦,陈宣,刘建秀. 结缕草属植物杂交后代杂种真实性鉴定——SRAP分子标记[J]. 草业学报, 2009, 18(1): 72-79.
[6]	薛丹丹,郑轶琦,王志勇,郭海林,陈宣,刘建秀. 结缕草属植物SRAP－PCR体系的建立和优化[J]. 草业学报, 2008, 17(6): 93-101.