兰州百合同源四倍体诱导及其基因组大小估算

doi:10.11686/cyxb2019425

草业学报 ›› 2020, Vol. 29 ›› Issue (3): 190-196.DOI: 10.11686/cyxb2019425

兰州百合同源四倍体诱导及其基因组大小估算

李淑洁^1,2,3, 张金文^2,3, 裴怀弟¹, 王红梅¹, 罗俊杰^1,*, 林玉红^1,*

1.甘肃省农业科学院生物技术研究所,甘肃兰州 730070;
2.甘肃省干旱生境作物学重点实验室,甘肃省作物遗传改良与种质创新重点实验室,甘肃兰州 730070;
3.甘肃农业大学农学院,甘肃兰州 730070

收稿日期:2019-09-29 出版日期:2020-03-20 发布日期:2020-03-20
通讯作者: *E-mail: sjsljjie@gsagr.ac.cn, ruby6146926@qq.com
作者简介:李淑洁(1980-),女,甘肃临夏人,副研究员,在读博士。E-mail: sjli2005@gsagr.ac.cn
基金资助:
国家自然科学基金地区基金项目(31960599),甘肃省农业科学院重点研发计划(2019GAAS019),甘肃省农业科学院科技支撑计划(2017GAAS36,2018GAAS01)和甘肃省农业科学院中青年基金(2017GAAS94)资助

Autotetraploid plant induction and the genome size estimation in Lanzhou lily (Lilium davidii var. unicolor)

LI Shu-jie^1,2,3, ZHANG Jin-wen^2,3, PEI Huai-di¹, WANG Hong-mei¹, LUO Jun-jie^1,*, LIN Yu-hong^1,*

1.Institute of Biotechnology, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China;
2.Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Key Lab of Crop Improvement and Germplasm Enhancement, Lanzhou 730070, China;
3.College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China

Received:2019-09-29 Online:2020-03-20 Published:2020-03-20

摘要/Abstract

摘要： 兰州百合(2n=2x=24)是重要的“药食同源”植物,进行其种质创新和基因组大小分析对于丰富其种质资源和生物学信息具有重要意义。以兰州百合的种子和试管小鳞茎为试验材料,通过秋水仙素浸泡诱导染色体加倍,经流式细胞仪倍性检测获得的同源四倍体植株分别有10和4株,四倍体诱导率分别为21.11%和33.27%。为了估算兰州百合基因组大小,以小麦品种“中国春”为内标,通过流式细胞仪测算兰州百合二倍体和四倍体植株中的DNA含量,得出兰州百合基因组为64.9 Gbp,是“中国春”核基因组的4倍。

关键词: 兰州百合, 秋水仙素, 同源多倍体, 流式细胞仪, 基因组大小

Abstract: Lanzhou lily (Lilium davidii var. unicolor, 2n=2x=24) is a well-known plant that is used both as food and for medicinal purposes. It is of great significance to breed elite germplasm and estimate its genome size. Autotetraploid induction in Lanzhou lily was accomplished in this study by soaking in different concentrations of colchicine, using seeds and tube-bulbs as the explants. Numbers of autotetraploids identified by flow cytometry assay among the plants derived from colchicine treated seeds and tube-bulbs were 10 and 4, respectively. The tetraploid induction rates were 21.11% for seeds and 33.27% for tube-bulbs. In order to estimate the genome size of Lanzhou lily, Triticum aestivum “Chinese spring” was used as an internal standard. The DNA content of both diploid and tetraploid Lanzhou lily plants was tested by flow cytometry. The genome size of Lanzhou lily was found to be 64.9 Gbp, four times greater than that of T. aestivum “Chinese spring”.

Key words: Lanzhou lily, colchicine, autotetraploid, flow cytometry, genome size

李淑洁, 张金文, 裴怀弟, 王红梅, 罗俊杰, 林玉红. 兰州百合同源四倍体诱导及其基因组大小估算[J]. 草业学报, 2020, 29(3): 190-196.

LI Shu-jie, ZHANG Jin-wen, PEI Huai-di, WANG Hong-mei, LUO Jun-jie, LIN Yu-hong. Autotetraploid plant induction and the genome size estimation in Lanzhou lily (Lilium davidii var. unicolor)[J]. Acta Prataculturae Sinica, 2020, 29(3): 190-196.

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兰州百合同源四倍体诱导及其基因组大小估算

Autotetraploid plant induction and the genome size estimation in Lanzhou lily (Lilium davidii var. unicolor)

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