Abstract: Seashore paspalum plays an important role in grassland systems in saline soil because of its strong salinity tolerance and other multiple stress resistance and high quality performance. P. vaginatum is a self-incompatible, sexual diploid. Cross-fertilization is difficult because its genome (D) is different from that of most other Paspalum species. P. vaginatum cv. adalayd was induced with colchicine in callus subculture medium, and embryogenic calli were obtained from immature inflorescences in order to explore the possibility of creating self-compatible somatic mutants. One thousand nine hundred and sixty plantlets from treatments with 250, 500, 1000, 1500, and 2000 mg/L colchicine for 24, 48, and 72 h on callus, and six hundred and eighty eight plantlets from the control were obtained. Characteristics of seed set from regenerated plants indicated that four seeds and three seeds were harvested from two different plants from treatments with 1000 mg/L colchicine for 24 h, and one seed from the treatment with 500 mg/L colchicine for 24 h. One seed per plot from three different plants of M₁ generation were sown, and every seed germinated. The three plants from seeds were named as SP2008-1, SP2008-2, and SP2008-3. Two hundred fifty three, thirty eight, and forty five self-compatible seeds from SP2008-1, SP2008-2, and SP2008-3 respectively were harvested, and weights per one thousand seeds were 0.471, 0.440, and 0.496 g respectively. The somatic chromosome number was 2n=20 in SP2008-1 and SP2008-2 and both were diploid. Somatic chromosome number 2n=20 was 86.7%, and somatic chromosome number 2n=40 was 13.3% in SP2008-3. SP2008-3 was a chimera mixed with diploid and tetraploid. RAPD analysis confirmed that SP2008-1, SP2008-2 and SP2008-3 plants were different from control adalayd plants at the molecular leve1. Two self-compatible diploids and one chimera with diploid and tetraploid somatic mutants were created using P. vaginatum cv. Adalayd callus induced with colchicine.