黄花蒿抗疟相关成分及抗氧化活性对施肥方式的响应

doi:10.11686/cyxb20140140

摘要/Abstract

摘要： 本研究探讨施肥对黄花蒿抗疟相关成分及抗氧化活性的影响,为科学施肥和提高黄花蒿产量品质提供理论依据。采用盆栽试验,设置不同施肥处理,于黄花蒿现蕾期分别采样测定根、茎、叶中的青蒿酸、去氧青蒿素、青蒿素、总黄酮含量及累积量,分析各器官乙醇提取液对DPPH·的清除率。结果表明,施肥显著促进黄花蒿生长,单株生物产量分别比不施肥增加 57.4%(无机肥)、91.6%(有机肥)和 92.3%(有机无机配施)。在黄花蒿体内,青蒿酸、去氧青蒿素、青蒿素、总黄酮的含量和累积量高低趋势均表现为:叶>茎>根,说明叶片是这些物质合成和储存的主要器官。此外,叶片的抗氧化活性 (DPPH·清除率)也最高,显著高于根系和茎,并且与总黄酮含量呈显著正相关。DPPH·清除率总体为不施肥处理最高,施肥后不同程度降低,施肥对总黄酮含量影响不大,但显著提高了青蒿酸、去氧青蒿素、青蒿素的含量和累积量(产量)及总黄酮的产量,其中有机无机配施和有机肥处理总体优于无机肥。说明在集约化种植黄花蒿的过程中,提倡施用有机肥是必要的。

Abstract: A pot experiment was carried out to study the changes in antimalarial compounds and antioxidation activities of Artemisia annua in response to fertilization to increase yields and to improve quality by rational fertilization. The plants were supplied with chemical fertilizer (CF),manure (M) or a mixture of chemical fertilizer and manure (CFM). The control (no fertilizer) was established in the same way except no fertilizer was added. Artemisic acid,dexyartemisinin,artemisinin,total flavonoids of leaves,stems and roots from A. annua were measured and then DPPH· (1,1-dipheny-l,2-picrylhyrazyl) scavenging rate of ethanol extracts during the bud period was analyzed. Fertilization promoted plant growth and the biomass was increased by 57.4% (CF),91.6% (M),and 92.3% (CFM) compared with the control. The concentration and accumulation of artemisic acid,deoxyartemisinin,artemisinin,and total flavonoids of A. annua changed in the following sequence: leaves>stems>roots,indicating that these compounds were formed and stored mainly in the leaves. Antioxidation activities expressed by the DPPH·scavenging rate,were also higher in leaves than those in roots and stems. There was a significant correlation between total flavonoids and the DPPH·scavenging rate. Fertilization,particularly CFM and M,decreased DPPH·scavenging rates but increased the concentration and accumulation of artemisic acid,dexyartemisinin,and artemisinin,even though total flavonoids changed little after fertilization. Therefore,it is necessary to advocate manure fertilization in the intensive cultivation of A. annua.

中图分类号:

S567.906

罗世琼，石安东，袁玲，黄建国. 黄花蒿抗疟相关成分及抗氧化活性对施肥方式的响应[J]. 草业学报, 2014, 23(1): 339-345.

LUO Shi-qiong,SHI An-dong,YUAN Ling,HUANG Jian-guo. Changes in antimalarial compounds and antioxidation activities of Artemisia annua in response to fertilization[J]. Acta Prataculturae Sinica, 2014, 23(1): 339-345.

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