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草业学报 ›› 2009, Vol. 18 ›› Issue (6): 226-234.

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叶绿素荧光技术在筛选光合突变体中的应用

郭玉朋1,郑霞2,王新宇1,曹孜义1,3*   

  1. 1.兰州大学生命科学院,甘肃 兰州730000;
    2.中国农业科学院麻类研究所,湖南 长沙 410205;

    3.甘肃农业大学生命科学技术学院,甘肃 兰州730070
  • 收稿日期:2008-11-03 出版日期:2009-12-20 发布日期:2009-12-20
  • 作者简介:郭玉朋(1975-),男,甘肃敦煌人,博士。E-mail: mlplml@sina.com

Application of a chlorophyll fluorescence technique for screening photosynthetic mutants

GUO Yu-peng1, ZHENG Xia2, WANG Xin-yu1, CAO Zi-yi1,3   

  1. 1.School of Life Sciences, Lanzhou University, Lanzhou 730000, China;
    2.Institute of Bast Fiber
    Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China;
    3.School of Life
    Science and Technology, Gansu Agricultural University, Lanzhou 730070, China
  • Received:2008-11-03 Online:2009-12-20 Published:2009-12-20

摘要: 叶绿素荧光技术作为光合作用研究的手段,在光合突变体筛选中起到了重要作用。使用该技术,大量光合突变体得以分离,并且随着分子生物学的进展,突变基因也被陆续克隆,同时利用这一技术的筛选方法也随着人们对光合作用机制认识的深入,不断得到了改进,本研究就这方面进展做以综述。

Abstract: As a basic technique in photosynthetic research, chlorophyll fluorescence is an important way to screen photosynthetic mutants. Many mutants have been isolated using this technique, and some genes have been identified in recent years. At the same time, screening method theories have also been developed with the accumulation of knowledge on photosynthesis. The development of screening technology is discussed in this article.

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