多浆旱生植物霸王液泡膜H+-PPase基因片段的克隆及序列分析

草业学报 ›› 2011, Vol. 20 ›› Issue (1): 119-124.

多浆旱生植物霸王液泡膜H⁺-PPase基因片段的克隆及序列分析

席杰军,伍国强,包爱科,王锁民^*

兰州大学草地农业科技学院,甘肃兰州 730020

出版日期:2011-02-22 发布日期:2011-02-22
通讯作者: E-mail: smwang@lzu.edu.cn
作者简介:席杰军(1982-),男,河南信阳人,在读博士。
基金资助:
国家973项目(2007CB108900),863计划项目(2006AA10Z126)和国家自然科学基金(30671488)资助。

Cloning and sequence analysis of a vacuolar H⁺-PPase gene fragment from Zygophyllum xanthoxylum

XI Jie-jun, WU Guo-qiang, BAO Ai-ke, WANG Suo-min

College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China

Online:2011-02-22 Published:2011-02-22

摘要/Abstract

摘要： 根据其他植物H⁺-PPase基因的保守序列设计一对简并性引物,以霸王叶片总RNA为模板,采用RT-PCR的方法扩增出H⁺-PPase基因片段并克隆到PUCm-T载体。阳性克隆经PCR鉴定后进行测序,序列分析结果表明,克隆到3个同源的序列片段(898 bp),编码299个氨基酸;所得序列与GenBank中注册的高等植物H⁺-PPase基因核苷酸序列的同源性均在69%以上、氨基酸序列的同源性达78%以上。

Abstract: Degenerate primers were designed based on conserved sequences of H⁺-Pase genes from other plants. Total RNA was extracted from the leaves of Zygophyllum xanthoxylum and the H⁺-PPase gene fragment was obtained by reverse transcription polymerase chain reaction (RT-PCR). It was cloned into PUCm-T vector and the positive clones identified by PCR were sequenced. Three H⁺-PPase gene fragments were gained from Z. xanthoxylum, each of which contains 898 bp and encodes a peptide of 299 amino acids. Similarity comparisons show that they share over 69% similarity in nucleotide sequence and over 78% similarity in amino acid sequence with those of other plant H⁺-PPase genes in GenBank.

中图分类号:

Q943.2

席杰军,伍国强,包爱科,王锁民. 多浆旱生植物霸王液泡膜H⁺-PPase基因片段的克隆及序列分析[J]. 草业学报, 2011, 20(1): 119-124.

XI Jie-jun, WU Guo-qiang, BAO Ai-ke, WANG Suo-min. Cloning and sequence analysis of a vacuolar H⁺-PPase gene fragment from Zygophyllum xanthoxylum[J]. Acta Prataculturae Sinica, 2011, 20(1): 119-124.

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