海盐胁迫下苏牧2号象草幼苗不同器官中阳离子分配与运输

草业学报 ›› 2012, Vol. 21 ›› Issue (5): 237-247.

海盐胁迫下苏牧2号象草幼苗不同器官中阳离子分配与运输

刘智微¹,钟小仙^1*,常盼盼^1,2,刘伟国^1,3

1.江苏省农业科学院畜牧研究所,江苏南京 210014;
2.南京农业大学动物科技学院,江苏南京 210095;
3.扬州大学动物科技学院,江苏扬州 225009

收稿日期:2011-09-13 出版日期:2012-05-25 发布日期:2012-10-20
通讯作者: E-mail:zhpansy@yahoo.com.cn
作者简介:刘智微(1986-),女,蒙古族,内蒙古赤峰人,实习员,硕士。E-mail:liuzhiweiwin2008@126.com
基金资助:
江苏省科技支撑计划项目(BE2010308)和江苏省自主创新项目(CX(11)2051)资助。

Cationic distribution and transportation in different organs of Pennisetum purpureum cv. Sumu No.2 seedling under sea-salt stress

LIU Zhi-wei¹, ZHONG Xiao-xian¹, CHANG Pan-pan^1,2, LIU Wei-guo^1,3

1.Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;
2.College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 225009, China;
3.College of Animal Science and Technology, Yangzhou Agricultural University, Yangzhou 210095, China

Received:2011-09-13 Online:2012-05-25 Published:2012-10-20

摘要/Abstract

摘要： 以象草苏牧2号和N51(对照)为材料,研究了不同海盐浓度胁迫下幼苗根、茎和叶中Na⁺、K⁺、Ca²⁺和Mg²⁺含量随胁迫时间延长的分配与运输规律。结果表明,苏牧2号和N51植株各部分Na⁺含量均随海盐浓度的升高而升高,K⁺、Ca²⁺、 Mg²⁺含量和K⁺/Na⁺均随海盐浓度的升高而降低。随胁迫时间的延长,10 g/L海盐浓度胁迫后,苏牧2号根中Na⁺含量显著高于N51。4～10 g/L海盐浓度胁迫后,苏牧2号茎中Na⁺含量总体上显著低于N51,叶中Na⁺含量苏牧2号均低于N51,但不同胁迫时间下差异不尽相同;苏牧2号根、茎中K⁺含量总体显著高于N51,苏牧2号和N51叶中K⁺含量高低则不尽相同;苏牧2号叶中K⁺/Na⁺总体显著高于N51;苏牧2号的S_K⁺/Na⁺ (茎/根)除4 g/L处理后第7天苏牧2号低于N51外,苏牧2号均高于N51,是其运输系数的1.02～2.19倍。表明苏牧2号的S_K⁺/Na⁺ (茎/根)高,使得根系能截留更多的Na⁺和向茎选择性运输更多的K⁺,提高茎中K⁺/Na⁺,同时其叶片中保持了高K⁺/Na⁺,从而减小植株受盐害程度,是苏牧2号耐盐性比N51增强的原因之一。

Abstract: With prolonged stress time under different sea-salt concentrations, Na⁺, K⁺, Ca²⁺, Mg²⁺ distribution and transportation in roots, stems and leaves of Sumu No.2 Napier grass seedlings were analyzed and compared with those of N51(CK). Na⁺ content increased but K⁺, Ca²⁺, Mg²⁺ content and the ratios of K⁺/Na⁺ decreased in all organs of Sumu No.2 and N51 as the sea-salt concentration increasd. With increasing time under 10 g/L sea-salt concentration stress, the Na⁺ content in roots of Sumu No.2 was higher than those of N51. Under 4-10 g/L sea-salt concentration stress, the Na⁺ content in stems of Sumu No.2 was significantly lower than that of N51, and that in leaves of Sumu No.2 was lower than in N51; the K⁺ content in roots and stems of Sumu No.2 was significantly higher than those of N51, while the K⁺ content in leaves of Sumu No.2 and N51 were not the same; The ratios of K⁺/Na⁺ in leaves of Sumu No.2 were higher than in N51; The transport selectivity of S_K⁺/Na⁺ (stem/root)of Sumu No.2 was 1.02-2.19 times that of N51, except that Sumu No.2 was lower than that of N51 after 7 d stress with 4 g/L sea-salt concentration. It appears that a major characteristic of the salt tolerance mechanism in Sumu No.2 seedlings is a high transport selectivity of S_K⁺/Na⁺ (stem/root)that can intercept more Na⁺ in roots and selectively transport more K⁺ from root to stem resulting in a higher K⁺/Na⁺ ratios in stems and leaves under sea-salt stress.

中图分类号:

刘智微,钟小仙,常盼盼,刘伟国. 海盐胁迫下苏牧2号象草幼苗不同器官中阳离子分配与运输[J]. 草业学报, 2012, 21(5): 237-247.

LIU Zhi-wei, ZHONG Xiao-xian, CHANG Pan-pan, LIU Wei-guo. Cationic distribution and transportation in different organs of Pennisetum purpureum cv. Sumu No.2 seedling under sea-salt stress[J]. Acta Prataculturae Sinica, 2012, 21(5): 237-247.

参考文献

[1] 沙伟, 滕兆岩, 王岩, 等. 星星草耐盐性研究进展[J]. 齐齐哈尔大学学报, 2007, 23(2): 93-99.
[2] 丁海荣, 洪立州, 王茂文, 等. 星星草耐盐生理机制及改良盐碱土壤研究进展[J]. 安徽农学通报, 2007, 13(16): 58-59.
[3] 王萍, 郭继勋. 盐碱化草地常见牧草耐盐机理研究[J]. 东北师范大学学报自然科学版, 1998, (3): 116-119.
[4] 杨明锋, 杨超, 侯文莲, 等. NaCl和KCl胁迫对碱蓬根和地上部分生长的效应[J]. 山东师范大学学报(自然科学版), 2002, 17(1): 68-72.
[5] 侯振安, 李品芳, 郭世文, 等. NaCl胁迫对苜蓿和羊草生长与水分利用的影响[J]. 中国农业科学, 2002, 35(7):894-900.
[6] 党瑞红, 周俊山, 范海. 海滨锦葵的抗盐特性[J]. 植物生理学通讯, 2008, 44(4): 635-638.
[7] 刘志华. 盐生植物獐毛耐盐基础生理的研究[J]. 衡水学院学报, 2007, 9(1): 5-9.
[8] 卜庆梅, 柏新富, 朱建军, 等. 盐胁迫条件下三角滨藜叶片中盐分的积累与分配[J]. 应用与环境生物学报, 2007, 13(2): 192-195.
[9] 王龙强, 米永伟, 蔺海明. 盐胁迫对枸杞属两种植物幼苗离子吸收和分配的影响[J]. 草业学报, 2011, 20(4): 129-136.
[10] 张永亮, 聂微微, 任秀珍, 等. 复盐胁迫下二种虉草K⁺、Na⁺吸收与运输的特点[J]. 中国草地学报, 2010, 32(3): 28-33.
[11] 王先宏, 蔡青, 杨清辉, 等. 象草(Pennisetum purpureum Schumach)的减数分裂及植物学特征[J]. 亚热带农业研究, 2005, 1(3): 7-10.
[12] Lowe A J, Thorpe W, Teale A, et al. Characterisation of germplasm accessions of Napier grass (Pennisetum purpureum and P. purpureum×P. glaucum Hybrids) and comparison with farm clones using RAPD[J]. Genetic Resources and Crop Evolution, 2003, 50: 121-132.
[13] 王晓敏, 张燕, 龚德勇. 能源植物象草的综合利用和栽培技术[J]. 河北农业科学, 2010, 14(4): 96-97.
[14] Nyambati E M, Sollenberger L E, Kunkle W E. Feed intake and lactation performance of dairy cows offered napier grass supplemented with legume hay[J]. Livestock Production Science, 2003, 83(2): 179-189.
[15] 刘智微, 钟小仙, 沈益新. 海盐胁迫对苏牧 2号象草抗氧化酶活性和MDA含量的影响[J]. 中国草地学报, 2011, 33(1): 24-29.
[16] 李品芳, 杨志成. NaCl胁迫高羊茅生长及K⁺、Na⁺吸收与运输的动态变化[J]. 草业学报, 2005, 14(4): 58-64.
[17] 张永亮, 聂微微, 任秀珍, 等. 复盐胁迫下二种虉草K⁺、Na⁺吸收与运输的特点[J]. 中国草地学报, 2010, 32(3): 28-33.
[18] Greenway H, Munns R. Mechanism of salt tolerance in non-halophytes[J]. Annual Review of Plant Physiology and Plant MolecularBiology, 1980, 31: 149-190.
[19] 於丙军, 刘友良. SOS基因家族与植物耐盐性[J]. 植物生理学通讯, 2004, 40(4): 409-413.
[20] 景艳霞, 袁庆. NaCl胁迫对苜蓿幼苗生长及不同器官中盐离子分布的影响[J]. 草业学报, 2011, 20(2): 134-139.
[21] Blumwald E. Sodium transport and salt tolerance in plants[J]. Current Opinion in Cell Biology, 2000, 12: 431-434.
[22] 陈菊培. 盐胁迫下植物细胞吸收Na⁺的可能途径[J]. 海南大学学报自然科学版, 2005, 23(4): 383-390.
[23] 朱义, 谭贵娥, 何池全, 等. 盐胁迫对高羊茅(Festuca arundinacea)幼苗生长和离子分布的影响[J]. 生态学报, 2007, 27(12): 5447-5454.
[24] 冯玉龙, 刘恩举, 崔臻祥. 根系温度对植物的影响-根温对植物代谢的影响[J]. 东北林业大学学报, 1995, 23(4): 94-99.
[25] 高瑞, 赵瑞华, 杨学军, 等. 盐分和温度对盐节木幼苗早期生长的影响[J]. 生态学报, 2009, 29(10): 5395-5405.
[26] 钟小仙, 张建丽, 张国正, 等. 海盐胁迫下象草体细胞突变体苗期生长及Na⁺、K⁺分配[J]. 中国草地学报, 2010, 32(1): 48-52.
[27] 邹丽娜, 周志宇, 颜淑云, 等. 盐分胁迫对紫穗槐幼苗生理生化特性的影响[J]. 草业学报, 2011, 20(3): 84-90.
[28] 赵昕, 赵敏桂, 谭会娟, 等. NaCl胁迫对盐芥和拟南芥K⁺、Na⁺吸收的影响[J]. 草业学报, 2007, 16(4): 21-24.
[29] 李子芳, 王玉国, 孙守钧. 植物应对盐胁迫的生理机制[J]. 河北农业科学, 2008, 12(3): 1-3.
[30] 朱小梅, 洪立洲, 王茂文, 等. NaCl胁迫下作物对离子的吸收及分配[J]. 中国土壤与肥料, 2010, 4: 1-4.
[31] 邹轶, 顾洪如, 钟小仙, 等. 海盐胁迫对海滨雀稗生长及植株体内阳离子含量的影响[J]. 草业科学, 2009, 26(4): 117-120.