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草业学报 ›› 2013, Vol. 22 ›› Issue (5): 145-154.DOI: 10.11686/cyxb20130517

• 研究论文 • 上一篇    下一篇

外源NO对NaCl胁迫下紫花苜蓿种子萌发及幼苗氧化损伤的影响

徐严1,2,魏小红1*,李兵兵1,曹丽1,唐志敏2   

  1. 1.甘肃农业大学生命科学技术学院,甘肃 兰州 730070;
    2.郴州市农业科学研究所,湖南 郴州 423042
  • 出版日期:2013-10-20 发布日期:2013-10-20
  • 通讯作者: E-mail:weixh@gsau.edu.cn
  • 作者简介:徐严(1987-),男,湖北石首人,硕士。
  • 基金资助:
    澳大利亚援助局资助项目(072-036007)资助。

Effects of exogenous nitric oxide on seed germination and seedling oxidative damage in Medicago sativa under NaCl stress

XU Yan1,2, WEI Xiao-hong1, LI Bing-bing1, CAO Li1, TANG Zhi-ming2   

  1. 1.Collage of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China;
    2.Chenzhou Institute of Agricultural Science, Chenzhou 423042, China
  • Online:2013-10-20 Published:2013-10-20

摘要: 1)利用浓度分别为0.1和1.0 mmol/L的外源NO供体硝普钠(sodium nitroprusside, SNP)浸泡紫花苜蓿种子48 h。在(25±1)℃下将每批50粒种子置于培养皿进行恒温光照培养,重复3次,连续10 d在培养皿中加入6 mL 0.15% NaCl处理液并统计日发芽率,以探讨NO对NaCl胁迫下紫花苜蓿种子萌发的影响。2)利用0.1和1.0 mmol/L浓度的外源NO供体SNP处理0.15% NaCl胁迫下的紫花苜蓿幼苗。试验设计了4个处理,分别为T1:对照组(CK)为蒸馏水;T2:0.15% NaCl;T3:0.1 mmol/L SNP+0.15% NaCl;T4:1.0 mmol/L SNP+0.15% NaCl。每个处理重复3次。分别于0 (胁迫前),2,4,6和8 d后采集生长状况一致的幼苗叶片测定游离脯氨酸含量、丙二醛(malondialdehyde, MDA)含量、过氧化氢(H2O2)含量、O2-产生速率、过氧化氢酶(catalase, CAT)、过氧化物酶(peroxidase, POD)、超氧化物歧化酶(superoxide dismutase,SOD)、叶绿素含量、可溶性糖含量。以探讨NO对NaCl胁迫下的紫花苜蓿幼苗叶片抗氧化系统的影响。结果表明,外源NO可以缓解NaCl胁迫对紫花苜蓿种子萌发的抑制作用,促进NaCl胁迫下紫花苜蓿种子活力,促进脯氨酸和可溶性糖含量的积累,能缓解NaCl胁迫引起的膜脂过氧化产物MDA含量的增加、缓解活性氧代谢引发的H2O2含量的增加及叶绿素的降解,抑制O2-产生速率,提高SOD、POD、CAT的活性。这种保护效应与NO的浓度明显相关,0.1 mmol/L SNP处理效果显著优于1.0 mmol/L SNP处理。

Abstract: 1) Medicago sativa seeds were soaked in two concentrations (0.1 and 1.0 mmol/L) of sodium nitroprusside (SNP) for 48 h. Samples of 50 seeds taken on 10 consecutive days were cultured in dishes under conditions of constant light and temperature at (25±1)℃.NaCl solution (6 mL 0.15%) was added to investigate the effects of exogenous nitric oxide on seed germination under NaCl stress. 2) The influence of the exogenous nitric oxide donor (SNP) at concentrations of 0.1 and 1.0 mmol/L on M. sativa seedling leaf oxidative damage under 0.15% NaCl stress were studied for 8 days of treatment. The treatments were, T1: CK (distilled water); T2: 0.15% NaCl; T3: 0.1 mmol/L SNP+0.15% NaCl; T4: 1.0 mmol/L SNP+0.15% NaCl. The results indicated that germination rate (P<0.01), germination energy, germination index (P<0.05) and vigor index (P<0.05) of M. sativa seeds were dramatically promoted by SNP treatments during germination under 0.15% NaCl stress. The activities of antioxidant enzymes such as superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT), were increased, which is consistent with the up-regulation of proline and soluble sugars, and with the down-regulation of O2- production, speed and content of malondialdehyde (MDA), H2O2 and chlorophyll in M. sativa seedling leaves compared with those under salt stress. Meanwhile, the protective ability of concentrations of 0.1 mmol/L was significantly better than those of 1.0 mmol/L.

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