贮藏温度对不同含水量老芒麦种子生理特性的影响

草业学报 ›› 2011, Vol. 20 ›› Issue (6): 101-108.

贮藏温度对不同含水量老芒麦种子生理特性的影响

朱萍¹,孔令琪¹,李高²,张晓媛¹,于晓娜¹,毛培胜^1*

1.中国农业大学草业科学系北京市草业科学重点实验室,北京 100193;
2.北京颐和园管理处,北京 100091

收稿日期:2010-12-23 出版日期:2011-06-25 发布日期:2011-12-20
通讯作者: E-mail:maops@cau.edu.cn
作者简介:朱萍(1987-),女,山东烟台人,在读硕士。E-mail:zhuping8687@163.com
基金资助:
现代牧草产业技术体系(nycytx-037)和十二五科技支撑课题(2011BAD17B01-02)资助。

Effect of moisture content on physiological characteristics of Elymus sibiricus seed under different storage temperature conditions

ZHU Ping¹, KONG Ling-qi¹, LI Gao², ZHANG Xiao-yuan¹, YU Xiao-na¹, MAO Pei-sheng¹

1.Department of grassland science, China Agricultural University, Beijing key laboratory of grassland science, Beijing 100193, China;
2.Summer Palace Management Office, Beijing 100091, China

Received:2010-12-23 Online:2011-06-25 Published:2011-12-20

摘要/Abstract

摘要： 试验以不同含水量(4%,10%,16%,22%,28%,34%,40%)老芒麦种子为材料,研究贮藏温度对老芒麦种子氧自由基、抗氧化酶及膜脂过氧化程度的影响,为探索种子劣变的内在机制奠定基础。结果表明,经过4℃低温和常温贮藏后,种子发芽率不同程度降低,含水量高的种子发芽率下降明显;随着含水量增加,死种子明显增多,超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量总体呈下降趋势。在低温贮藏条件下,抗坏血酸过氧化物酶(APX)活性、O₂^·- 产生速率随含水量的升高不断下降,在含水量达到28%时,APX活性、O₂^·- 产生速率开始上升。在常温贮藏条件下,种子含水量在22%之后,APX活性升高,O₂^·- 产生速率总体呈下降趋势,但含水量在16%时,O₂^·- 产生速率突然升高,发芽率降低到0%,不正常、新鲜未发芽种子和死种子明显增多。在低水分(4%～16%)条件下,超氧阴离子自由基的积累可能是引起种子劣变的关键,而高含水量种子的活力下降,并非与自由基积累及脂质过氧化作用相关。

Abstract: The experiments were conducted to study the influence of storage temperature on superoxide anion radical, antioxidant enzyme activities and lipid peroxidation in Elymus sibiricus seeds with different moisture content (4%, 10%, 16%, 22%, 28%, 34%, 40%), and try to explore the mechanism of seed deterioration with different moisture content. The results showed that seed germination rate decreased gradually after low and room temperature storage and germination capability of seeds with higher moisture content (16%~40%) lost significantly. The percent of dead seeds increased, SOD and MDA content generally decreased with the moisture content improved. APX (ascorbate peroxidase) and superoxide anion production rate decreased with the moisture content increasing under the low temperature. In 28% moisture content, APX and superoxide anion production rate began to rise. After stored under room temperature, APX increased in 22% moisture content, superoxide anion production rate generally declined. But in 16% moisture content, superoxide anion production rate rose dramatically and seed germination rate suddenly decreased to zero, abnormal seeds and dead seeds obviously increased. In low moisture content(4%～16%), superoxide anion radical accumulation could be the key to cause seed deterioration. But the vigor reduction of seeds with high moisture content was not related to free radical accumulation and lipid peroxidation.

中图分类号:

S816.33
S548

朱萍,孔令琪,李高,张晓媛,于晓娜,毛培胜. 贮藏温度对不同含水量老芒麦种子生理特性的影响[J]. 草业学报, 2011, 20(6): 101-108.

ZHU Ping, KONG Ling-qi, LI Gao, ZHANG Xiao-yuan, YU Xiao-na, MAO Pei-sheng. Effect of moisture content on physiological characteristics of Elymus sibiricus seed under different storage temperature conditions[J]. Acta Prataculturae Sinica, 2011, 20(6): 101-108.

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