菊芋内生固氮菌分离、鉴定及特性研究

草业学报 ›› 2011, Vol. 20 ›› Issue (6): 157-163.

菊芋内生固氮菌分离、鉴定及特性研究

孟宪法,隆小华,康健,王雪晴,刘兆普^*

南京农业大学资源与环境科学学院江苏省海洋生物学重点实验室,江苏南京 210095

收稿日期:2010-09-25 出版日期:2011-06-25 发布日期:2011-12-20
通讯作者: E-mail:sea@ njau.edu.cn
作者简介:孟宪法(1987-),男,山东青岛人,硕士。E-mail:2011203009@njau.edu.cn
基金资助:
公益性行业(农业)科研专项经费项目(200903001-05),国家“948”项目(2012-S9),国家科技支撑项目(2009BADA3B04-8和2011BAD13B09),江苏省科技支撑项目(BE2011368和BE2010305),教育部博士点基金(20100097120016)和中央高校基本业务费项目(Y0201100249)资助。

Isolation and identification of endogenic nitrogen-fixing bacteria in the roots of Jerusalem artichoke (Helianthus tuberosus)

MENG Xian-fa, LONG Xiao-hua, KANG Jian, WANG Xue-qing, LIU Zhao-pu

College of Resources and Environmental Sciences, Jiangsu Provincial Key Laboratory of Marine Biology, Nanjing Agricultural University, Nanjing 210095, China

Received:2010-09-25 Online:2011-06-25 Published:2011-12-20

摘要/Abstract

摘要： 通过气相色谱,结合乙炔还原法,对菊芋根系的内生固氮菌进行了分离和鉴定。结果表明,筛选得到的7株具有固氮酶活性的菌株,分别属于根瘤菌属,寡养单胞菌属,假单胞菌属和肠杆菌属。菌株之间固氮酶活性相差较大,固氮酶活性较高的菌株较少,100 nmol/(mL·h)以上的只有cho1和cho9两株菌。菌株cho4的耐盐性较高,可以在盐浓度为7%的条件下正常生长。分离得到的菌株中,有5株具有解磷活性,其中以菌株cho7的活性最高,为63.84 μg/mL。菌株全部都具有分泌生长素的能力,但差异较大,分泌IAA浓度最高的是cho2,达到13.49 μg/mL。

Abstract: Endogenic nitrogen-fixing bacteria (ENB) from the roots of Jerusalem artichoke (Helianthus tuberosus) were isolated and identified using acetylene reduction assay (ARA) combined with gas chromatography (GC). The seven ENB strains isolated belonged to Rhizobium (2 strains), Stenotrophomonas (3 strains), Pseudomonas (1 strain) and Enterobacter (1 strain). Significant differences in nitrogenase activity were found among the ENB strains. The nitrogenase activities of two strains, cho1 and cho9, were higher than 100 nmol/(mL·h). Cho4 strain had the strongest salt-tolerance and could grow normally in condition of 7% NaCl. Five strains showed the power of phosphate solubilization and strain cho7 had the highest ability (63.84 μg/mL). All the ENB strains could excrete IAA, but there was a significant difference in the ability to do so, and strain cho2 had the highest activity (13.49 μg/mL).

中图分类号:

Q945.13

孟宪法,隆小华,康健,王雪晴,刘兆普. 菊芋内生固氮菌分离、鉴定及特性研究[J]. 草业学报, 2011, 20(6): 157-163.

MENG Xian-fa, LONG Xiao-hua, KANG Jian, WANG Xue-qing, LIU Zhao-pu. Isolation and identification of endogenic nitrogen-fixing bacteria in the roots of Jerusalem artichoke (Helianthus tuberosus)[J]. Acta Prataculturae Sinica, 2011, 20(6): 157-163.

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