裸仁美洲南瓜种皮性状遗传规律研究

草业学报 ›› 2011, Vol. 20 ›› Issue (3): 205-210.

裸仁美洲南瓜种皮性状遗传规律研究

薛应钰^1,2,师桂英³,徐秉良^1,2*,陈荣贤⁴

1.甘肃农业大学草业学院草业生态系统教育部重点实验室中-美草地畜牧业可持续发展研究中心(甘肃农业大学),甘肃兰州 730070;
2.甘肃省作物遗传改良与种质创新重点实验室,甘肃兰州 730070;
3.甘肃农业大学农学院,甘肃兰州 730070;
4.甘肃省武威金苹果有限责任公司,甘肃武威 733000

收稿日期:2010-10-13 出版日期:2011-03-25 发布日期:2011-06-20
通讯作者: E-mail:xubl@gsau.edu.cn
作者简介:薛应钰(1978-),男,甘肃镇原人,讲师,在读博士。E-mail:xueyy@gsau.edu.cn
基金资助:
国家自然科学基金项目(30671267)和甘肃省科技厅中青年基金项目(3YS051-A25-015)资助。

A study on the genetic law of seed coat characters in hull-less Cucurbita pepo

XUE Ying-yu^1,2, SHI Gui-ying³, XU Bing-liang^1,2, CHEN Rong-xian⁴

1.College of Grassland Science, Gansu Agricultural University/Key Laboratory of Grassland Ecosystem, Gansu Agricultural University, Ministry of Education,Sino-U.S. Centers for Grassland Ecosystem Sustainability, Lanzhou 730070, China;
2.Gansu Key Laboratory of Crop Genetic&Germplasm Enhancement,Lanzhou 730070, China;
3.College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China;
4.Wuwei Golden Apple Co., Ltd.,Wuwei 733000, China

Received:2010-10-13 Online:2011-03-25 Published:2011-06-20

摘要/Abstract

摘要： 采用3个有壳美洲南瓜自交系04FC409-4、03N-122-19、04LAg-26-2和3个无壳美洲南瓜自交系04GD112-7、1N-275-7、04LAg-26-28作亲本,通过正、反交及回交组配,对其杂交组合(04FC409-4×04GD112-7、03N-122-19×1N-275-7、04LAg-26-2×04LAg-26-28)的F₁、F₂、BC₁和BC₂各世代的种皮分离比率进行统计分析,结果表明,F₁代种皮性状表现一致,无论正、反交均表现为有壳;F₂代无论正、反交都分离出有壳和无壳2种性状,且分离比率接近3∶1;BC₁(以有壳的自交系作父本进行回交)种皮均表现为有壳,BC₂(以无壳的自交系作父本进行回交)种皮则表现出有壳和无壳的分离,分离比率接近1∶1。根据经典遗传学原理对种皮的遗传效应进行了研究,初步推断出:美洲南瓜种皮的有壳与无壳受一对核基因控制,有壳对无壳为显性。

Abstract: The seed coat trait of Cucurbita pepo was classified into two types, seed-hulled and seed-naked. Experiments were performed to study the inheritance pattern. Three inbreeds with hulled seed, 04FC409-4, 03N-122-19 and 04LAg-26-2, and three inbreeds with hull-less seed, 04GD112-7, 1N-275-7 and 04LAg-26-28, were used as the parental lines in the mating patterns 04FC409-4 and 04GD112-7, 03N-122-19 and 1N-275-7, 04LAg-26-2 and 04LAg-26-28. In the F₁ generations, all the plants were seed-hulled. However, in the F₂ generations, segregation of hulled and hull-less seeds showed the ratio of almost 3 to 1. For the backcrosses, all the BC₁ and F₁ (the inbreed with hulled seed used as recurrent parent) generations appeared seed-hulled. But in the BC₂ and F₁ (the inbreed with hull-less seed as recurrent parent) generations, segregation of hulled and hull-less seeds showed the ratio of almost 1 to 1. So it was concluded that each of the hulled seed and hull-less seed in C. pepo was controlled by one pair of different nuclear genes and “seed-hulled” was dominant to “seed-hull-less”.

中图分类号:

S642.13
Q943

薛应钰,师桂英,徐秉良,陈荣贤. 裸仁美洲南瓜种皮性状遗传规律研究[J]. 草业学报, 2011, 20(3): 205-210.

XUE Ying-yu, SHI Gui-ying, XU Bing-liang, CHEN Rong-xian. A study on the genetic law of seed coat characters in hull-less Cucurbita pepo[J]. Acta Prataculturae Sinica, 2011, 20(3): 205-210.

参考文献

[1] 刘玉梅, 高智明, 王健, 等. 裸仁南瓜籽及南瓜籽油的营养成分研究[J]. 食品工业科技, 2010, 31(6): 313-316.
[2] 肖文一. 饲料南瓜一地多种高产栽培技术试验报告[J]. 哈尔滨商业大学学报(自然科学版),1980, (3): 34-35.
[3] 张仲保. 无种壳西葫芦巨仁[J]. 中国蔬菜, 2007, (2): 62-62.
[4] Teppner H. Cururbita pepo (Cucurbitaceae): History,seed coat types,thin coated seeds and their genetics[J]. Phyton-international Journal of Experimental Botany, 2000, 40: 1-42.
[5] 陈荣贤, 吕生全, 孙有鑫, 等. 天然无壳瓜籽的化学组成及其分析[J]. 甘肃农业科技, 1988, (3): 32-33.
[6] 贾楠. 裸仁瓜与新型液态奶牛饲料——研制者田然升谈新型液态饲料[J].当代畜禽与饲料, 2009, (8): 56-57.
[7] 张松林, 田侠, 董庆士, 等. 沙漠绿洲多功能高分子植物生长调节剂籽瓜增产和品质改善研究[J]. 中国沙漠, 2006, 26(3): 503-506.
[8] 何亚娟, 潘学标. 甘肃省民勤绿洲种植结构与水资源利用的研究[J]. 中国生态农业学报, 2003, 11(4): 121-123.
[9] 陈吉斌, 刘胜祥, 王明忠. 甘肃民勤植物资源调查[J]. 黑龙江农业科学, 2007, (5): 104-105.
[10] 于红茹, 苏国辉. 我国籽用南瓜现状及前景分析[J]. 北方园艺, 2008, (4): 54.
[11] 谢文刚, 张新全, 陈永霞. 鸭茅杂交种的SSR分子标记鉴定及其遗传变异分析[J].草业学报, 2010, 19(2): 212-217.
[12] 孟秋峰, 王毓洪, 周焕兴, 等. 园艺植物保守器官分类研究进展[J].宁波农业科技, 2008, (4): 21-23.
[13] 路覃坦, 张金政, 孙国峰, 等.四种国产野生无髯鸢尾种子休眠类型的研究[J].草业科学, 2009, 18(2): 130-137.
[14] 刘志文, 毛玮, 周芳彬, 等. 甘蓝型油菜种皮颜色的遗传分析[J]. 华北农学报, 2008, 23(2): 84-87.
[15] 严明理, 刘忠松, 官春云. 芥菜型油菜种皮颜色和叶片颜色的遗传[J].作物研究, 2008, 22(3): 157-159.
[16] 胡晓辉, 石运庆, 苗华荣, 等. 花生种皮颜色遗传及相关品质分析[J]. 江西农业学报, 2009, 21(3): 27-28.
[17] Branch W D. 花生种皮深红色遗传的研究[J]. 花生科技, 1999, (1): 37-39.
[18] 陈廷文. 稻谷黑米种皮的遗传特性研究[J]. 四川农业大学学报, 1995, 13(4): 498-505.
[19] 姚金保, 姚国才, 杨学明, 等.青海黑小麦种皮色泽的初步遗传分析[J]. 麦类作物学报, 2007, 27(5): 791-793.
[20] Wei L F, von Becherer E P. Zur factoreanalyse der testa aus beldung beim kurbis[J]. Berlin Deutsch Botanische Gesselschaft, 1950, 63: 147-148.
[21] Robinson R W, Munger H M, Whitaker T W, et al. Genes of the Cucurbitaceae[J].Hortscience, 1976, 11: 554-568.
[22] Stuart S G. Comparative biochemical and genetic studies of testa development in normal and hull-less phenotypes of pumpkin[D]. PhD thesis,University of New Hampshire, 1983.
[23] 周祥麟. 裸仁南瓜的育成及其遗传规律的探讨[J]. 山西农业科学, 1983, (12): 21-22.
[24] 周祥麟. 裸仁南瓜的育成及其遗传性研究[J]. 园艺学报, 1987, 14(2): 115-118.