异源表达WZY2-1基因提高拟南芥植株抗旱性

doi:10.11686/cyxb2017293

草业学报 ›› 2018, Vol. 27 ›› Issue (6): 92-99.DOI: 10.11686/cyxb2017293

异源表达WZY2-1基因提高拟南芥植株抗旱性

强治全¹, 杨文博², 张帅¹, 于正阳¹, 史学英¹, 王鑫¹, 朱维宁³, 张林生^1,*

1.西北农林科技大学生命科学学院,旱区作物逆境生物学国家重点实验室, 陕西杨凌712100;
2.河南省农业科学院粮食作物研究所,河南郑州 450002;
3.西北大学生命科学学院, 陕西西安710069

收稿日期:2017-06-26 修回日期:2017-11-22 出版日期:2018-06-20 发布日期:2018-06-20
通讯作者: * E-mail: linszhang@nwsuaf.edu.cn
作者简介:强治全(1990-),男,甘肃庆阳人,在读硕士。E-mail:qiangsir0934@qq.com
基金资助:
国家自然科学基金(31671608)和旱区作物逆境生物学国家重点实验室基金(CSBAA2017008)资助

Heterologous expression of WZY2-1 affects drought resistance of Arabidopsis plants

QIANG Zhi-quan¹, YANG Wen-bo², ZHANG Shuai¹, YU Zheng-yang¹, SHI Xue-ying¹, WANG Xin¹, ZHU Wei-ning³, ZHANG Lin-sheng^1,*

1.College of Life Science, State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University, Yangling 712100, China;
2. Henan Academy of Agricultural Sciences, Zhengzhou 540002, China;
3. College of Life Science, Northwest University, Xi’an 710069, China

Received:2017-06-26 Revised:2017-11-22 Online:2018-06-20 Published:2018-06-20
Contact: * E-mail: linszhang@nwsuaf.edu.cn

摘要/Abstract

摘要： 脱水素是受细胞失水相关环境诱导表达的一类蛋白家族。所有的脱水素都含有保守的K片段,这段保守序列可以形成两亲性的α-螺旋,这种结构在脱水素发挥功能中起主要作用。小麦WZY2-1基因含有9个K片段,属于K9型的脱水素,能够被低温、干旱和盐胁迫诱导表达。在本实验中,纯化了WZY2-1蛋白,通过体外LDH酶保护实验发现,WZY2-1蛋白具有保护LDH酶活性的功能。通过亚细胞定位分析发现,WZY2-1蛋白主要定位于细胞核和细胞膜。为了进一步说明WZY2-1基因的功能,获得了转WZY2-1基因的拟南芥植株,通过干旱胁迫条件下转基因植株的生理指标和表型分析,发现转WZY2-1基因的拟南芥植株具有较强的抗旱能力。因此,推测小麦WZY2-1基因在植物干旱胁迫条件下具有重要功能。

关键词: 脱水素, 亚细胞定位, LDH酶活性, 小麦

Abstract: Dehydrins (DHNs) belong to a protein family whose expression may be induced or enhanced by environmental stresses that lead to cell dehydration. All DHNs contain at least one motif, or K-fragment, which can form an amphipathic helix. This structure play an important role in DHN function. The wheat gene WZY2-1, a K9-type DHN gene, can be activated by low temperature, drought and salt stress. In this work, the WZY2-1 recombinant protein was purified. In vitro lactate dehydrogenase (LDH) assays revealed that WZY2-1 had a potent protective effect during freezing-thawing temperature stresses. Subcellular localization showed that WZY2-1 was localized in the nucleus and cell membrane. To further elucidate the function of WZY2-1 in plants, transgenic Arabidopsis plants that overexpress WZY2-1 were tested. The transgenic Arabidopsis lines showed a better tolerance to drought stress than unmodified. This suggests that the WZY2-1 gene may also play an important role in drought tolerance in wheat.

Key words: dehydrins, subcellular localization, LDH activity, wheat

强治全, 杨文博, 张帅, 于正阳, 史学英, 王鑫, 朱维宁, 张林生. 异源表达WZY2-1基因提高拟南芥植株抗旱性[J]. 草业学报, 2018, 27(6): 92-99.

QIANG Zhi-quan, YANG Wen-bo, ZHANG Shuai, YU Zheng-yang, SHI Xue-ying, WANG Xin, ZHU Wei-ning, ZHANG Lin-sheng. Heterologous expression of WZY2-1 affects drought resistance of Arabidopsis plants[J]. Acta Prataculturae Sinica, 2018, 27(6): 92-99.

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异源表达WZY2-1基因提高拟南芥植株抗旱性

Heterologous expression of WZY2-1 affects drought resistance of Arabidopsis plants

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