外源纤维素酶对发酵全混合日粮营养价值、发酵品质和酶活性的动态影响

doi:10.11686/cyxb2018602

草业学报 ›› 2019, Vol. 28 ›› Issue (9): 123-134.DOI: 10.11686/cyxb2018602

外源纤维素酶对发酵全混合日粮营养价值、发酵品质和酶活性的动态影响

陈光吉¹, 吴佳海^{1, *}, 尚以顺¹, 张蓉¹, 何廷章², 吴彦³, 娄芬⁴, 熊俊⁴, 甘小波⁴, 陈荫琼⁴, 王普昶¹, 韩永芬⁵, 李世歌¹, 舒建虹¹

1.贵州省农业科学院草业研究所,贵州贵阳 550006;
2.绥阳县枧坝镇农业服务中心,贵州绥阳 563307;
3.绥阳县草地工作站,贵州绥阳 563300;
4.大方县农业农村局,贵州大方 551600;
5.贵州金农富平生态农牧科技有限公司,贵州贵阳 550006

收稿日期:2018-09-13 修回日期:2019-03-11 出版日期:2019-09-20 发布日期:2019-09-20
通讯作者: *E-mail: 1848266168@qq.com
作者简介:陈光吉(1990-),男,贵州遵义人,助理研究员,硕士。E-mail: cgjgz09@126.com
基金资助:
贵州省科技计划项目(黔科合成果[2017]4117),贵州省农业科学院科技创新项目(黔农科院科技创新[2017]01号),贵州省科技计划项目 (黔科合支撑[2017]2591),国家重点研发计划(2017YFD0502101-3),贵州省农业科学院农业科技改革与创新服务园区项目,贵州省优秀青年科技人才培养对象专项(黔科合人字(2015)02号),贵州省科学技术基金(黔科合J字 [2015]2080号),科技创新人才团队建设项目(黔科合平台人才[2016]5617),贵州省科技计划项目(黔科合服企[2018]4001)和贵州省科技计划项目(黔科合人才[2016]5713)资助

Dynamic effects of exogenous fibrolytic enzyme supplementation on nutritive value, fermentation quality and enzyme activities of fermentation total mixed ration

CHEN Guang-ji¹, WU Jia-hai^1,*, SHANG Yi-shun¹, ZHANG Rong¹, HE Ting-zhang², WU Yan³, LOU Fen⁴, XIONG Jun⁴, GAN Xiao-bo⁴, CHEN Yin-qiong⁴, WANG Pu-chang¹, HAN Yong-fen⁵, LI Shi-ge¹, SHU Jian-hong¹

1.Guizhou Institute of Prataculture, Guizhou Academy of Agricultural Sciences, Guiyang 550006, China;
2.Agricultural Service Center, Jian Ba Town, Suiyang County, Suiyang 563307, China;
3.Grassland Workstation of Suiyang County, Suiyang 563300, China;
4.Agriculture and Rural Bureau of Dafang County, Dafang 551600, China;
5.Guizhou Jinnong Fuping Ecological Agriculture and Animal Husbandry Technology Co., Ltd., Guiyang 550006, China

Received:2018-09-13 Revised:2019-03-11 Online:2019-09-20 Published:2019-09-20
Contact: *E-mail: 1848266168@qq.com

摘要/Abstract

摘要： 本试验旨在探究外源纤维素酶(EFE)添加到发酵全混合日粮(FTMR)中对其营养价值、发酵品质和酶活性的影响,并找出适宜添加水平。采用完全随机试验设计,设置5个纤维素酶添加水平(0、0.4、0.8、1.6、3.2 g·kg^-1 DM),分别测定底物发酵的第7、14、35、49、70天的概略养分、可溶性碳水化物(WSC)、发酵参数和4种酶活力。结果表明,1)纵向看,随发酵时间的延长,底物的干物质(DM)、中性洗涤纤维(NDF)、酸性洗涤纤维(ADF)和可溶性碳水化合物含量,以及4种酶活力均呈显著下降趋势(P<0.05),而乙酸含量则呈显著上升趋势(P<0.05)。2)横向看,随着EFE添加量的提高,发酵一定时间后, DM、NDF、ADF和WSC含量以及pH值、氨态氮含量、羧基肽酶活力和酸性蛋白酶活力呈显著下降趋势(P<0.05),而乙酸含量、纤维素酶活力、和木聚糖活力呈上升趋势(P<0.05),发酵第35天后趋于稳定,0.8和1.6 g·kg^-1 EFE添加组表现出较低的pH值、NDF含量和ADF含量(P<0.05),以及较高的纤维素酶和木聚糖酶活力(P<0.05);由此,本试验发现,添加EFE可促进FTMR中纤维类物质的降解、有机酸的积累和自身纤维类物质的酶活力,抑制羧基肽酶活力和酸性蛋白酶活力,减少蛋白质的损失,且存在添加剂量效应,适宜添加量为0.8~1.6 g·kg^-1 DM。

关键词: FTMR, 外源纤维素酶, 酶活力, 动态影响

Abstract: The objective of this study was to determine the effect of exogenous fibrolytic enzyme (EFE) supplementation in fermentated total mixed ration (FTMR) on the nutritional value, fermentation quality and enzyme activities, and to find out the optimal level of EFE addition. A completely randomized experimental design was adopted, and 5 levels of EFE (0, 0.4, 0.8, 1.6 and 3.2 g·kg^-1 dry matter) were added to FTMR as the 5 experimental treatments. At 7, 14, 35, 49, 70 days after initiation of fermentation, major nutrients, water soluble carbohydrate (WSC), fermentation parameters and activities of four enzymes were determined. It was found: 1) With increased fermentation time, the dry matter (DM), neutral detergent fiber (NDF), acid detergent fiber (ADF), and WSC contents and the activities of the four enzymes monitored showed a decrease (P<0.05), while acetic acid accumulated with time (P<0.05). 2) With increased EFE supplementation, the DM, NDF, ADF and WSC contents, pH value, ammonia nitrogen content, and the activities of carboxyl peptidase and acid protease decreased (P<0.05) until day 35 and then stabilised, while the acetic acid content, and cellulase and xylanase activities increased and stabilized over the same time period (P<0.05). At day 35, the EFE supplementation treatments of 0.8 and 1.6 g·kg^-1 showed lower pH values, NDF and ADF contents, and higher activity of cellulase and xylanase (P<0.05). It was found that the addition of EFE promoted fiber degradation and the accumulation of organic acids through enzymatic activity, inhibited the activities of carboxypeptidase and acidic protease, and reduced the loss of protein in FTMR. The dose effect was additive and the optimum EFE dose was 0.8-1.6 g·kg^-1 DM.

Key words: FTMR, exogenous fibrolytic enzymes, enzyme activity, dynamic effects

陈光吉, 吴佳海, 尚以顺, 张蓉, 何廷章, 吴彦, 娄芬, 熊俊, 甘小波, 陈荫琼, 王普昶, 韩永芬, 李世歌, 舒建虹. 外源纤维素酶对发酵全混合日粮营养价值、发酵品质和酶活性的动态影响[J]. 草业学报, 2019, 28(9): 123-134.

CHEN Guang-ji, WU Jia-hai, SHANG Yi-shun, ZHANG Rong, HE Ting-zhang, WU Yan, LOU Fen, XIONG Jun, GAN Xiao-bo, CHEN Yin-qiong, WANG Pu-chang, HAN Yong-fen, LI Shi-ge, SHU Jian-hong. Dynamic effects of exogenous fibrolytic enzyme supplementation on nutritive value, fermentation quality and enzyme activities of fermentation total mixed ration[J]. Acta Prataculturae Sinica, 2019, 28(9): 123-134.

参考文献

[1] Silva T H, Takiya C S, Vendramini T H A, et al. Effects of dietary fibrolytic enzymes on chewing time, ruminal fermentation, and performance of mid-lactating dairy cows. Animal Feed Science and Technology, 2016, 221: 35-43.
[2] Pinosrodriguez J M, Moreno R, Gonzalez S S, et al. Effects of exogenous fibrolytic enzymes on ruminal fermentation and digestibility of total mixed rations fed to lambs. Animal Feed Science and Technology, 2008, 142: 210-219.
[3] Avellaneda J H, Pinosrodriguez J M, Gonzalez S S, et al. Effects of exogenous fibrolytic enzymes on ruminal fermentation and digestion of Guinea grass hay. Animal Feed Science and Technology, 2009, 149: 70-77.
[4] Giraldo L A, Tejido M L, Ranilla M J, et al. Influence of direct-fed fibrolytic enzymes on diet digestibility and ruminal activity in sheep fed a grass hay-based diets. Journal of Animal Science, 2014, 86: 1617-1623.
[5] Song S D, Chen G J, Guo C H, et al. Effects of exogenous fibrolytic enzyme supplementation to diets with different NFC/NDF ratios on the growth performance, nutrient digestibility and ruminal fermentation in Chinese domesticated black goats. Animal Feed Science and Technology, 2018, 236(1): 170-177.
[6] Ren Z H, Liu J Y.Discussion far forage-saving husbandry developing mode based on beef cattle and mutton breeding. Chinese Journal of Animal Science, 2016, 52(22): 20-23.
任智慧, 刘俊盈. 基于肉牛肉羊养殖的节粮型畜牧业发展模式探讨模式. 中国畜牧杂志, 2016, 52(22): 20-23.
[7] Shioya S.Future prospects of TMR center based on self-supplying feed. Japanese Journal of Grassland Science, 2008, 54(2): 178-181.
[8] Wang F, Nishino N.Resistance to aerobic deterioration of total mixed ration silage: Effect of ration formulation, air infiltration and storage period on fermentation characteristics and aerobic stability. Journal of the Science of Food and Agriculture, 2008, 88(1): 133-140.
[9] Cao Y, Takahashi T, Horiguchi K.Effects of addition of food by-products on the fermentation quality of a total mixed ration with whole crop rice and its digestibility, preference, and rumen fermentation in sheep. Animal Feed Science and Technology, 2009, 151(1): 1-11.
[10] Jia C W, Yuan X J, Xiao S H, et al. Effect of substituting hulless barley straw for tall fescue on early fermentation quality and aerobic stability of mixed-ration silage in Tibet. Acta Prataculturae Sinica, 2016, 25(4): 179-187.
贾春旺, 原现军, 肖慎华, 等. 青稞秸秆替代苇状羊茅对全混合日粮青贮早期发酵品质及有氧稳定性的影响. 草业学报, 2016, 25(4): 179-187.
[11] Wang Y, Yuan X J, Guo G, et al. Fermentation and aerobic stability of mixed ration forages in Tibet. Acta Prataculturae Sinica, 2014, 23(6): 95-102.
王勇, 原现军, 郭刚, 等. 西藏不同饲草全混合日粮发酵品质和有氧稳定性的研究.草业学报, 2014, 23(6): 95-102.
[12] Cui Y Z, Huang K H, Zhang K C, et al. Effects of different lactic acid bacteria agents on mycotoxin content in fermentation total mixed ration. China Dairy Cattle, 2015, 9: 9-13.
崔彦召, 黄克和, 张克春, 等. 不同乳酸菌剂对发酵全混合日粮霉菌毒素含量的影响. 中国奶牛, 2015, 9: 9-13.
[13] Qiu X Y, Yuan X J, Guo G, et al. Effects of molasses and acetic acid on fermentation and aerobic stability of total mixed ration silage in Tibet. Acta Prataculturae Sinica, 2014, 23(6): 111-118.
邱小燕, 原现军, 郭刚, 等. 添加糖蜜和乙酸对西藏发酵全混合日粮青贮发酵品质及有氧稳定性影响. 草业学报, 2014, 23(6): 111-118.
[14] Wang Q Z, Huang G Y, Zhou J H, et al. Effects of enzymes on nutritional value of mulberry silage. Shanghai Journal of Animal Husbandry and Veterinary Medicine, 2016, 3: 24-25.
王启芝, 黄光云, 周俊华, 等. 酶制剂对桑树青贮营养价值的影响试验. 上海畜牧兽医通讯, 2016, 3: 24-25.
[15] Liu Q W, Wang F, Wei L M, et al. Effects of juncao silage supplemented by enzyme preparation on production performance and serum biochemical parameters of Hainan black goats. China Feed, 2018, 11: 62-65.
刘圈伟, 王峰, 魏立民, 等. 复合酶制剂青贮菌草对海南黑山羊生产性能及血清生化指标的影响. 中国饲料, 2018, 11: 62-65.
[16] Zeng H, Qiu Y L, Li L, et al. Effects of different combination enzyme preparations on nutritional value of straw microstores. China Feed, 2019, 1: 80-84.
曾辉, 邱玉朗, 李林, 等. 不同组合酶制剂对秸秆微贮饲料营养价值的影响. 中国饲料, 2019, 1: 80-84.
[17] Colombatto D, Mould F L, Bhat M K, et al. Use of fibrolytic enzymes to improve the nutritive value of ruminant diets. A biochemical and in vitro rumen degradation assessment. Animal Feed Science and Technology, 2003, 107: 201-209.
[18] Feng Y L, Wang J Q, Yang H J, et al. Beef cattle raising standard, NY/T 815-2004. Beijing: Ministry of Agriculture of China, 2004.
冯仰廉, 王加启, 杨红建, 等. NY/T 815-2004, 肉牛饲养标准. 北京: 中国农业部, 2004.
[19] Chen G J, Song S D, Peng Z L, et al. Determination of appropriate level of exogenous fibrolytic enzyme supplementation for substrates with different non-fiber carbohydrate/neutral detergent fiber ratios based on in vitro gas production. Acta Prataculturae Sinica, 2017, 26(7): 116-127.
陈光吉, 宋善丹, 彭忠利, 等. 体外产气法研究不同NFC/NDF底物条件下外源纤维素酶的适宜添加水平. 草业学报, 2017, 26(7): 116-127.
[20] Zhang L Y.Feed analysis and quality testing technology (2nd Edition). Beijing：China Agricultural University Press, 2003.
张丽英. 饲料分析及质量检测技术(第2版). 北京:中国农业大学出版社, 2003.
[21] Wang R R, Wang H L, Liu X, et al. Effects of different additives on fermentation characteristics and protein degradation of green tea grounds silage. Asian-Australasian Journal of Animal Science, 2011, 24(5): 616-622.
[22] Weatherburn M W.Phenol-hypochlorite reaction for determination of ammonia. Analytical Chemistry, 1967, 39(8): 971-974.
[23] Jurgen N K, Lloyd D, Fricker O V, et al. Hyperproinsulinaemia in obese fat/fat mice associated with a carboxypeptidase e mutation which reduces enzyme activity. Nature Genetics, 1995, 10(2): 135-142.
[24] Yang Z M, Wang Q, Qu S M, et al. Dynamics of three enzyme activities in alfalfa silage. Chinese Journal of Grassland, 2012, 34(1): 113-115.
杨智明, 王琴, 曲善民, 等. 苜蓿青贮过程中三种酶活性动态研究. 中国草地学报, 2012, 34(1): 113-115.
[25] Isac M D, García M A, Aguilera J F, et al. A comparative study of nutrient digestibility, kinetics of digestion and passage and rumen fermentation pattern in goats and sheep offered medium quality forages at the maintenance level of feeding. Archiv Für Tierernaehrung, 1994, 46(1): 37-50.
[26] Woolford M, Bolsen K, Peartt L A.Studies on the aerobic deterioration of whole-crop silages. Journal of Agricultural Science, 1982, 98(3): 529-535.
[27] Zhang F, Cai H Y, Wang Z G, et al. Dynamic changes of fermentation quality and nutritional components of different varieties of whole plant barley silage. China Dairy Cattle, 2014, 23(24): 1-8.
张放, 蔡海莹, 王志耕, 等. 不同品种全株饲用大麦青贮发酵品质及其营养成分动态变化研究. 中国奶牛, 2014, 23(24): 1-8.
[28] Jia T T, Wu Z, Yu Z, et al. Effects of different types of Lactobacillus additives on quality and aerobic stability of oat silage. Prataculturae Science, 2018, 5: 1266-1272.
贾婷婷, 吴哲, 玉柱, 等. 不同类型乳酸菌添加剂对燕麦青贮品质和有氧稳定性的影响. 草业科学, 2018, 5: 1266-1272.
[29] Shao T, Ohba N, Shimojo M, et al. Effects of adding glucose, sorbic acid and pre-fermented juices on the fermentation quality of guineagrass (Panicumjournal maximum Jacq.) silages. Asian Australasian of Animal Sciences, 2004, 17(6): 808-813.
[30] Zhang L, Yu C Q, Shao T, et al. Effect of different rates of ethanol additive on fermentation quality of napiergrass (Pennisetum purpureum). Asian Australasian of Animal Sciences, 2011, 24(5): 636-642.
[31] Van Man N, Wiktorsson H.Effect of molasses on nutritional quality of cassava and gliricidia tops silage. Asian Australasian of Animal Sciences, 2002, 15(9): 1294-1299.
[32] Nadeau E M G, Buxton D R, Lindgren E, et al. Kinetics of cell-wall digestion of orchardgrass and alfalfa silages treated with cellulase and formic acid1, 2. Journal of Dairy Science, 1996, 79(12): 2207-2216.
[33] Van Soest P J. Nutritional ecology of the ruminants, comstock publishing associates. Ithaca, New York,USA：Cornell University Press, 1994.
[34] Mertens D R, Loften J R.The effect of starch on forage fiber digestion kinetics in vitro. Journal of Dairy Science, 1980, 63(9): 1437-1446.
[35] Amjed M, Jung H G, Donker J D.Effect of alkaline hydrogen peroxide treatment on cell wall composition and digestion kinetics of sugarcane residues and wheat straw. Journal of Animal Science, 1992, 70(9): 2877-2884.
[36] Ma Q H, Li M, Zhou H L.Effects of cellulase on silage quality and carbohydrate content of royal grass. Heilongjiang Animal Science and Veterinary Medicine, 2011, 2: 79-81.
马清河, 李茂, 周汉林. 纤维素酶对王草青贮品质和碳水化合物含量的影响. 黑龙江畜牧兽医, 2011, 2: 79-81.
[37] Weinberg Z G.Preservation of forage crops by solid-state lactic acid fermentation-ensiling. Current Developments in Solid-state Fermentation. New York: Springer, 2008: 443-467.
[38] Liang X Y, Ji Y, Yi J, et al. Effects of mixing ratio and additives on the quality of mixed silage of chicory and silage corn. Acta Prataculturae Sinica, 2018, 27(2): 173-181.
梁小玉, 季杨, 易军, 等. 混合比例和添加剂对菊苣与青贮玉米混合青贮品质的影响. 草业学报, 2018, 27(2): 173-181.
[39] Chen L.Effects of replacement of whole-plant corn with oat and common vetch on the fermentation quality and aerobicstability of total mixed ration. Nanjing: Nanjing Agricultural University, 2014.
陈雷. 燕麦和箭舌豌豆替代全株玉米对TMR发酵品质和有氧稳定性的影响. 南京: 南京农业大学, 2014.
[40] Silva J S, Ribeiro K G, Pereira O G, et al. Nutritive value and fermentation quality of palisadegrass and stylo mixed silages. Animal Science Journal, 2018, 89(1): 72-78.
[41] Kang S, Wanapat M, Nunoi A, et al. Effect of urea and molasses supplementation on quality of cassava top silage. Journal of Animal and Feed Science, 2018, 27(1): 74-80.
[42] McDonald P. Animal nutrition. Canada: Pearson education, 2002.
[43] Nsereko V L, Beauchemin K A, Morgavi D P, et al. Effect of a fibrolytic enzyme preparation from Trichoderma longibrachiatum on the rumen microbial population of dairy cows. Canadian Journal of Microbiology, 2002, 48(1): 14-20.
[44] Wang D, Lin J Q.Research progress in direct bioconversion of cellulosic resources to fuel ethanol production. Journal of Shandong Agricultural University, 2002, 33(4): 525-529.
王丹, 林建强. 直接生物转化纤维素类资源生产燃料乙醇的研究进展. 山东农业大学学报, 2002, 33(4): 525-529.
[45] Ren H W, Liu F F, Wang L, et al. Effects of cellulase on the mixed silage quality of dried maize straw and cabbage waste and an analysis of its bacterial community. Chinese Journal Applied Environmental Biology, 2018, 24(5): 1097-1106.
任海伟, 刘菲菲, 王莉, 等. 纤维素酶对干玉米秸秆/白菜废弃物混贮品质的影响及细菌群落解析. 应用与环境生物学报, 2018, 24(5) : 1097-1106.
[46] Li J, Zhou P, Liu H, et al. Synergism of cellulase, xylanase, and pectinase on hydrolyzing sugarcane bagasse resulting from different pretreatment technologies. Bioresource Technology, 2014, 155(5): 258-265.
[47] Dean D B, Adesogan A T, Krueger N, et al. Effect of fibrolytic enzymes on the fermentation characteristics, aerobic stability, and digestibility of bermudagrass silag. Journal of Dairy Science, 2005, 88(3): 994-1003.
[48] Mendoza G D, Loeracorral O, Plataperez F X, et al. Considerations on the use of exogenous fibrolytic enzymes to improve forage utilization. The Scientific World Journal, 2014: 247-437.
[49] Wang M Q.Discussion on cellulase enzymatic washing process. Silk, 2003, (10): 44.
王美琴. 纤维素酶酶洗工艺的探讨. 丝绸, 2003, (10): 44.
[50] Mitsuaki O, McDonal P. A review of the changes in nitrogen noun compounds of herbage during ensilage. Journal of Science of Food Agriculture, 1978, 29: 497-505.

外源纤维素酶对发酵全混合日粮营养价值、发酵品质和酶活性的动态影响

Dynamic effects of exogenous fibrolytic enzyme supplementation on nutritive value, fermentation quality and enzyme activities of fermentation total mixed ration

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 2

编辑推荐

Metrics

[1]	陈光吉, 宋善丹, 彭忠利, 王普昶, 吴佳海, 王小利, 郭春华, 王子苑, 高彦华, 李小冬, 柏雪, 付锡三. 体外产气法研究不同NFC/NDF底物条件下外源纤维素酶的适宜添加水平[J]. 草业学报, 2017, 26(7): 116-127.
[2]	黄勤楼, 钟珍梅, 黄秀声, 陈钟佃, 冯德庆, 夏友国. 纤维素降解菌的筛选及在狼尾草青贮中使用效果评价[J]. 草业学报, 2016, 25(4): 197-203.