尼龙袋法评价香蕉叶单宁对瘤胃降解特性的影响

doi:10.11686/cyxb2019375

摘要/Abstract

摘要： 旨在探究香蕉叶单宁对奶牛瘤胃降解特性的影响和评价香蕉叶的饲喂价值。样品分别为苜蓿干草组(100%的苜蓿干草)、苜蓿干草和香蕉叶混合组(50%苜蓿干草+50%香蕉叶)、香蕉叶组(100%香蕉叶)、苜蓿干草组(100%的苜蓿干草)+6%聚乙二醇(6% PEG)、苜蓿干草和香蕉叶混合组(50%苜蓿干草+50%香蕉叶)+6%聚乙二醇(6% PEG)、香蕉叶组(100%香蕉叶)+6%聚乙二醇(6% PEG)。测定样品中的营养成分含量,使用3头安装了瘤胃瘘管的荷斯坦奶牛进行半体内试验,用尼龙袋法测定上述样品在瘤胃内0、2、4、8、12、24、48、72 h的干物质(DM)、粗蛋白质(CP)、中性洗涤纤维(NDF)、酸性洗涤纤维(ADF)的降解率,得到各营养物质的动态降解参数。结果表明,相较于苜蓿干草,香蕉叶干物质和粗蛋白的快速降解部分、慢速降解部分、慢速降解部分的降解速率、有效降解率是显著低于苜蓿干草的(P<0.001),香蕉叶和苜蓿的NDF的有效降解率没有显著差异。添加了PEG后,香蕉叶和香蕉叶苜蓿混合组的快速降解部分和有效降解率显著高于未添加PEG的两组(P<0.001)。结果表明香蕉叶中的单宁会抑制香蕉叶的瘤胃降解率,因此在使用香蕉叶调制反刍动物的饲料时需要控制单宁的含量。

关键词: 单宁, 香蕉叶, 瘤胃降解率, 聚乙二醇

Abstract: The purpose of this study was to investigate the effects of tannin from banana leaves on the rumen degradation characteristics in dairy cows and to evaluate the feeding value of banana leaves. The treatments comprised alfalfa (100% alfalfa), alfalfa and banana leaf mixed (50% alfalfa+50% banana leaves), banana leaf (100% banana leaves), alfalfa with polyethylene glycol (PEG) (alfalfa+6% PEG), alfalfa and banana leaf mixed with PEG (alfalfa+banana leaves in a 1∶1 mixture+6% PEG), banana leaf group with PEG (banana leaves+6% PEG). In situ dry matter (DM), neutral detergent fiber (NDF) and crude protein (CP) degradability were determined by incubating the conserved forages in 3 rumen cannulated Holstein cows for 0, 2, 4, 8, 12, 24, 48 and 72 h. The results indicated that compared to alfalfa, banana leaves had lower rapidly degraded fraction (a), slowly degraded fraction (b), fractional disappearance rate (c) and effective degradability (ED, P<0.001) of DM and CP. However, the ED of NDF was similar for alfalfa and banana leaves. Both banana leaves and the alfalfa-banana leaf mixed forage with PEG had greater slowly degraded fractions and ED of DM, NDF and CP, than the two groups without PEG (P<0.001). In summary, the results indicated that tannins in banana leaves inhibited ruminal degradation of the banana leaves, and thus their level of inclusion in mixed diets needs to be controlled when fed to ruminants.

Key words: tannin, banana leaf, rumen degradability, PEG

李志威, 赵静雯, 沈思聪, 罗佳璇, 赵国琦, 黄倩倩. 尼龙袋法评价香蕉叶单宁对瘤胃降解特性的影响[J]. 草业学报, 2019, 28(12): 114-123.

LI Zhi-wei, ZHAO Jing-wen, SHEN Si-cong, LUO Jia-xuan, ZHAO Guo-qi, HUANG Qian-qian. Effects of tannins in banana leaves on in situ digestion in dairy cows[J]. Acta Prataculturae Sinica, 2019, 28(12): 114-123.

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