高羊茅FaRVE8基因的克隆、亚细胞定位及表达分析

doi:10.11686/cyxb2019452

草业学报 ›› 2020, Vol. 29 ›› Issue (7): 60-69.DOI: 10.11686/cyxb2019452

高羊茅FaRVE8基因的克隆、亚细胞定位及表达分析

罗维¹, 舒健虹², 刘晓霞², 王子苑², 牟琼², 王小利^2,*, 吴佳海^1,3,*

1.贵州大学动物科学学院高原山地动物遗传育种与繁殖教育部重点实验室,贵州贵阳550025;
2.贵州省农业科学院草业研究所,贵州贵阳550006;
3.贵州省农业科学院果树科学研究所,贵州贵阳550006

收稿日期:2019-10-23 修回日期:2019-12-20 出版日期:2020-07-20 发布日期:2020-07-20
通讯作者: *E-mail: 848266168@qq.com, wangxiaolizhenyuan@126.com
作者简介:罗维(1993-),女,贵州松桃人,在读硕士。E-mail: 1291928810@qq.com
基金资助:
国家自然科学基金(31860674)资助

Cloning, subcellular localization and expression analysis of the RVE8 gene from Festuca arundinacea

LUO Wei¹, SHU Jian-hong², LIU Xiao-xia², WANG Zi-yuan², MU Qiong², WANG Xiao-li^2,*, WU Jia-hai^1,3,*

1. School of Animal Science, Guizhou University, Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guiyang 550025, China;
2. Institute of Prataculture, Guizhou Academy of Agricultural Sciences, Guiyang 550006, China;
3. Institute of Fruit Research, Guizhou Academy of Agricultural Sciences, Guiyang 550006, China

Received:2019-10-23 Revised:2019-12-20 Online:2020-07-20 Published:2020-07-20

摘要/Abstract

摘要： 生物钟是一个复杂的调控网络,在环境的不断变化中促进植物的生长,REVEILLE8 (RVE8)是生物钟的重要基因。为探讨其分子机理,采用RT-PCR和RACE方法,克隆高羊茅叶片FaRVE8基因,结果显示,FaRVE8全长为1881 bp,有1236 bp的开放阅读框,编码411个氨基酸,同属于MYB样因子。遗传进化树表明其与禾本科植物二穗短柄草、节节麦、大麦的同源蛋白亲缘关系较近。利用荧光定量分析高羊茅叶片中FaRVE8在不同光照处理下的表达特征,结果表明,FaRVE8在不同光照处理下均有表达,且呈现出明显的昼夜节律。亚细胞定位显示FaRVE8定位在细胞核中,可能在细胞核中发挥重要作用。以上研究表明,FaRVE8在调节生物节律中有重要作用,为进一步探讨FaRVE8基因的功能和分子调控机制奠定基础。

关键词: 高羊茅, FaRVE8, 基因克隆, 表达分析

Abstract: The circadian clock is a complex regulatory network that promotes plant growth in the ever-changing environment. REVEILLE8 (RVE8) is an important gene in the circadian clock. In order to explore the molecular mechanism, the FaRVE8 gene of leaves from Festuca arundinacea was cloned by RT-PCR and RACE. It was found that the full length of FaRVE8 was 1881 bp, the open reading frame was 1236 bp, encoding 411 amino acids, which belonged to a MYB-like factor. The phylogenetic analysis showed that it is closely related to Brachypodium distachyon, Aegilops tauschii, and Hordeum vulgare. Real-time fluorescence quantitative analysis of FaRVE8 expression in F. arundinacea leaves under different light treatments showed that FaRVE8 was expressed under different light treatments and showed obvious circadian rhythm. Subcellular localization indicated that FaRVE8 is localized in the nucleus, and FaRVE8 may play an important role in the nucleus. These above studies indicate that the FaRVE8 gene plays an important role in regulating biological rhythms, laying the foundation for further study of the function and molecular regulation mechanisms of the FaRVE8 gene.

Key words: Festuca arundinacea, FaRVE8, gene cloning, expression analysis

罗维, 舒健虹, 刘晓霞, 王子苑, 牟琼, 王小利, 吴佳海. 高羊茅FaRVE8基因的克隆、亚细胞定位及表达分析[J]. 草业学报, 2020, 29(7): 60-69.

LUO Wei, SHU Jian-hong, LIU Xiao-xia, WANG Zi-yuan, MU Qiong, WANG Xiao-li, WU Jia-hai. Cloning, subcellular localization and expression analysis of the RVE8 gene from Festuca arundinacea[J]. Acta Prataculturae Sinica, 2020, 29(7): 60-69.

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高羊茅FaRVE8基因的克隆、亚细胞定位及表达分析

Cloning, subcellular localization and expression analysis of the RVE8 gene from Festuca arundinacea

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