Pathogen identification from soft rot disease of onion bulbs in Jiayuguan, Gansu province

doi:10.11686/cyxb20130419

Abstract

Abstract: A severe soft rot disease of onion (Allium cepa) bulbs occurred in Jiayuguan, Gansu province. The causal agent was isolated and identified based on morphological characteristics of two strains on KB culture medium and on molecular characters. The 16S rDNA region of the two strains had homoeology with the known species Serratia grimesii and Pectobacterium carotovorum subsp. carotovorum from the GenBank database. The sequence lengths were 1 379 bp and 1 386 bp, respectively with a maximum similarity of 99％and 100%. Both strains caused soft rot disease on onion bulbs in pathogenicity tests. The disease index from roots was higher than that from the tops and P. carotovorum subsp. carotovorum was more pathogenic than S. grimesii. The pathogens causing soft rot disease on onion bulbs in Jiayuguan, Gansu province were P. carotovorum subsp. carotovorum and S. grimesii. This is the first report of S. grimesii causing soft rot disease on onion bulbs and this study should provide a theoretical basis for controlling this disease.

CLC Number:

LV He-ping, WEI Hui, QI Yong-hong, ZHANG Yan-mei, CAO Su-fang, YANG Fa-rong, LI Min-quan, SHEN Hui-min. Pathogen identification from soft rot disease of onion bulbs in Jiayuguan, Gansu province[J]. Acta Prataculturae Sinica, 2013, 22(4): 153-159.

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